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Understanding how cellular morphology, gene expression, and spatial context jointly shape tissue function is a central challenge in biology. Image-based spatial transcriptomics technologies now provide high-resolution measurements of cell…

Quantitative Methods · Quantitative Biology 2026-02-17 Zhenglun Kong , Mufan Qiu , John Boesen , Xiang Lin , Sukwon Yun , Tianlong Chen , Manolis Kellis , Marinka Zitnik

Inspection of tissues using a light microscope is the primary method of diagnosing many diseases, notably cancer. Highly multiplexed tissue imaging builds on this foundation, enabling the collection of up to 60 channels of molecular…

Training a neural network with a large labeled dataset is still a dominant paradigm in computational histopathology. However, obtaining such exhaustive manual annotations is often expensive, laborious, and prone to inter and Intra-observer…

Computer Vision and Pattern Recognition · Computer Science 2021-11-03 Chetan L. Srinidhi , Seung Wook Kim , Fu-Der Chen , Anne L. Martel

The early detection and nuanced subtype classification of non-small cell lung cancer (NSCLC), a predominant cause of cancer mortality worldwide, is a critical and complex issue. In this paper, we introduce an innovative integration of…

Image and Video Processing · Electrical Eng. & Systems 2024-10-08 Salma Hassan , Hamad Al Hammadi , Ibrahim Mohammed , Muhammad Haris Khan

Medical image annotation is essential for diagnosing diseases, yet manual annotation is time-consuming, costly, and prone to variability among experts. To address these challenges, we propose an automated explainable annotation system that…

Computer Vision and Pattern Recognition · Computer Science 2025-02-26 Basma Nasir , Tehseen Zia , Muhammad Nawaz , Catarina Moreira

Automated segmentation of anatomical sub-regions with high precision has become a necessity to enable the quantification and characterization of cells/ tissues in histology images. Currently, a machine learning model to analyze…

Image and Video Processing · Electrical Eng. & Systems 2023-06-05 Hosein Barzekar , Hai Ngu , Han Hui Lin , Mohsen Hejrati , Steven Ray Valdespino , Sarah Chu , Baris Bingol , Somaye Hashemifar , Soumitra Ghosh

Recent advances in whole slide imaging (WSI) technology have led to the development of a myriad of computer vision and artificial intelligence (AI) based diagnostic, prognostic, and predictive algorithms. Computational Pathology (CPath)…

Segmentation and classification of large numbers of instances, such as cell nuclei, are crucial tasks in digital pathology for accurate diagnosis. However, the availability of high-quality datasets for deep learning methods is often limited…

Computer Vision and Pattern Recognition · Computer Science 2024-10-21 Laura Gálvez Jiménez , Christine Decaestecker

For diagnosing melanoma, hematoxylin and eosin (H&E) stained tissue slides remains the gold standard. These images contain quantitative information in different magnifications. In the present study, we investigated whether deep…

Tissues and Organs · Quantitative Biology 2019-04-15 Peizhen Xie , Ke Zuo , Yu Zhang , Fangfang Li , Mingzhu Yin , Kai Lu

Histopathological imaging is vital for cancer research and clinical practice, with multiplexed Immunofluorescence (MxIF) and Hematoxylin and Eosin (H&E) providing complementary insights. However, aligning different stains at the cell level…

Image and Video Processing · Electrical Eng. & Systems 2024-10-02 Jun Jiang , Raymond Moore , Brenna Novotny , Leo Liu , Zachary Fogarty , Ray Guo , Markovic Svetomir , Chen Wang

Automatic cell segmentation in microscopy images works well with the support of deep neural networks trained with full supervision. Collecting and annotating images, though, is not a sustainable solution for every new microscopy database…

Computer Vision and Pattern Recognition · Computer Science 2020-07-06 Youssef Dawoud , Julia Hornauer , Gustavo Carneiro , Vasileios Belagiannis

Although the U-Net architecture has been extensively used for segmentation of medical images, we address two of its shortcomings in this work. Firstly, the accuracy of vanilla U-Net degrades when the target regions for segmentation exhibit…

Foundation models (FMs) are transforming computational pathology by offering new ways to analyze histopathology images. However, FMs typically require weeks of training on large databases, making their creation a resource-intensive process.…

Image and Video Processing · Electrical Eng. & Systems 2026-01-27 Till Nicke , Daniela Schacherer , Jan Raphael Schäfer , Natalia Artysh , Antje Prasse , André Homeyer , Andrea Schenk , Henning Höfener , Johannes Lotz

Most recently, the pathology diagnosis of cancer is shifting to integrating molecular makers with histology features. It is a urgent need for digital pathology methods to effectively integrate molecular markers with histology, which could…

Image and Video Processing · Electrical Eng. & Systems 2023-06-28 Xiaofei Wang , Stephen Price , Chao Li

For many biological image segmentation tasks, including topological knowledge, such as the nesting of classes, can greatly improve results. However, most `out-of-the-box' CNN models are still blind to such prior information. In this paper,…

Computer Vision and Pattern Recognition · Computer Science 2018-08-22 Marie Piraud , Anjany Sekuboyina , Bjoern H. Menze

Digital Pathology is a cornerstone in the diagnosis and treatment of diseases. A key task in this field is the identification and segmentation of cells in hematoxylin and eosin-stained images. Existing methods for cell segmentation often…

Computer Vision and Pattern Recognition · Computer Science 2025-01-10 Fabian Hörst , Moritz Rempe , Helmut Becker , Lukas Heine , Julius Keyl , Jens Kleesiek

Manually annotating nuclei from the gigapixel Hematoxylin and Eosin (H&E)-stained Whole Slide Images (WSIs) is a laborious and costly task, meaning automated algorithms for cell nuclei instance segmentation and classification could…

Computer Vision and Pattern Recognition · Computer Science 2024-07-29 João D. Nunes , Diana Montezuma , Domingos Oliveira , Tania Pereira , Jaime S. Cardoso

Machine-learning-assisted cancer subtyping is a promising avenue in digital pathology. Cancer subtyping models, however, require careful training using expert annotations so that they can be inferred with a degree of known certainty (or…

Large pretrained transformers are increasingly being developed as generalised foundation models which can underpin powerful task-specific artificial intelligence models. Histopathology foundation models show great promise across many tasks,…

Image and Video Processing · Electrical Eng. & Systems 2024-09-10 Jack Breen , Katie Allen , Kieran Zucker , Lucy Godson , Nicolas M. Orsi , Nishant Ravikumar

Recent advances in computational pathology have leveraged vision-language models to learn joint representations of Hematoxylin and Eosin (HE) images with spatial transcriptomic (ST) profiles. However, existing approaches typically align HE…

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