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Fast 3D super-resolution imaging is essential for decoding rapidly occurring biological processes. Encoding single molecules to their respective planes enable simultaneous multi-plane super-resolution volume imaging. This saves the…

Biological Physics · Physics 2016-08-04 Partha Pratim Mondal

Three-dimensional (3D) nano-printing via two-photon polymerization offers unparalleled design flexibility and precision, thereby enabling rapid prototyping of advanced micro-optical elements and systems, including hybrid achromats,…

Optics · Physics 2026-02-25 Florian Lux , Aybuke Calikoglu , Çağlar Ataman

High-numerical-aperture (NA) oblique plane microscopy enables noninvasive fluorescence imaging of subcellular dynamics without requiring radical sample modification. However, performance degrades at depth in multicellular specimens as…

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

We present a 3D-printing-based design to produce wire-guided liquid microfilms that can be used for versatile spectroscopic applications. We demonstrate the ability of our instrument to provide optically useful liquid microfilms with highly…

Chemical Physics · Physics 2025-07-04 Matthew J. Silverstein , Yasashri Ranathunga , Yuki Kobayashi

Particle tracking velocimetry in 3D is becoming an increasingly important imaging tool in the study of fluid dynamics, combustion as well as plasmas. We introduce a dynamic discrete tomography algorithm for reconstructing particle…

Fluid Dynamics · Physics 2015-01-23 Andreas Alpers , Peter Gritzmann , Dmitry Moseev , Mirko Salewski

Recent development of lensless imagers has enabled three-dimensional (3D) imaging through a thin piece of optics in close proximity to a camera sensor. A general challenge of wide-field lensless imaging is the high computational complexity…

Optics · Physics 2021-08-17 Feng Tian , Junjie Hu , Weijian Yang

We present a method to capture both 3D shape and spatially varying reflectance with a multi-view photometric stereo (MVPS) technique that works for general isotropic materials. Our algorithm is suitable for perspective cameras and nearby…

Computer Vision and Pattern Recognition · Computer Science 2020-01-22 Min Li , Zhenglong Zhou , Zhe Wu , Boxin Shi , Changyu Diao , Ping Tan

Molecular dipoles near interfaces emit highly directional radiation due to near-field interactions, making surface-bound fluorophores sensitive probes of local physicochemical changes. We introduce smart coverslips, stably coated with…

We design a multiscopic vision system that utilizes a low-cost monocular RGB camera to acquire accurate depth estimation. Unlike multi-view stereo with images captured at unconstrained camera poses, the proposed system controls the motion…

Computer Vision and Pattern Recognition · Computer Science 2021-08-21 Weihao Yuan , Rui Fan , Michael Yu Wang , Qifeng Chen

The structure and dynamics of isolated nanosamples in free flight can be directly visualized via single-shot coherent diffractive imaging using the intense and short pulses of X-ray free-electron lasers. Wide-angle scattering images even…

A three-dimensional (3D) scanning velocimetry system is developed to quantify the 3D configurations of particles and their surrounding volumetric, three-component velocity fields. The approach uses a translating laser sheet to rapidly scan…

Fluid Dynamics · Physics 2021-08-04 Matt K. Fu , Isabel A. Houghton , John O. Dabiri

Multiphoton microscopy (MPM) has gained enormous popularity over the years for its capacity to provide high resolution images from deep within scattering samples1. However, MPM is generally based on single-point laser-focus scanning, which…

Optics · Physics 2018-12-14 Devin R. Beaulieu , Ian G. Davison , Thomas G. Bifano , Jerome Mertz

In optical imaging, achieving high resolution often comes at the expense of a shallow depth of field. This means that when using a standard microscope, any minor movement of the object along the optical axis can cause the image to become…

The application of dynamic light scattering to soft matter systems has strongly profited from advanced approaches such as the so-called modulated 3D cross correlation technique (mod3D-DLS) that suppress contributions from multiple…

Soft Condensed Matter · Physics 2022-03-15 Antara Pal , Peter Holmqvist , Andrea Vaccaro , Peter Schurtenberger

Photonic integration on plastic substrates enables emerging applications ranging from flexible interconnects to conformal sensors on biological tissues. Such devices are traditionally fabricated using pattern transfer, which is complicated…

3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…

Image and Video Processing · Electrical Eng. & Systems 2019-11-28 Yang Jiao , Mo Weng , Mei Yang

Multiphase flows where particles, bubbles, or droplets are suspended in a fluid govern critical processes in biology, medicine, materials processing, and geophysics. However, observing their microscale dynamics in opaque systems has…

Physics-driven discovery in an autonomous experiment has emerged as a dream application of machine learning in physical sciences. Here we develop and experimentally implement a deep kernel learning workflow combining the correlative…

Materials Science · Physics 2022-11-24 Kevin M. Roccapriore , Sergei V. Kalinin , Maxim Ziatdinov