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Microscopy has been key to tremendous advances in science, technology, and medicine, revealing structure and dynamics across time and length scales. However, combining high spatial and temporal resolution in a non-invasive, label-free…

The expansion of viewing angle is a crucial factor in holographic displays implemented with a spatial light modulator having a finite space-bandwidth. The enhanced-NA Fresnel hologram reconstructs a holographic image at an angle larger than…

Optics · Physics 2023-08-24 Byung Gyu Chae

Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…

Image and Video Processing · Electrical Eng. & Systems 2025-01-28 Tao Liu , Bingyang Wang , JiangTao Zhao , Maik Kahnt , Fucai Zhang

We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence. Our technique achieves super-resolved imaging by temporally modulating the…

Non-destructive nano-imaging of the internal structure of solid matter is only feasible using hard X-rays due to their high penetration. The highest resolution images are achieved at synchrotron radiation sources (SRF), offering superior…

For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent…

Optics · Physics 2017-05-31 Chao Zuo , Jiasong Sun , Jiaji Li , Jialin Zhang , Anand Asundi , Qian Chen

By exploiting the quantised nature of light, we demonstrate a sub-shot-noise scanning optical transmittance microscope. Our microscope demonstrates, with micron scale resolution, a factor of improvement in precision of 1.76(9) in…

Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…

The viewing-angle enlargement of a holographic image is a crucial factor for realizing the holographic display. The numerical aperture (NA) of digital hologram other than a pixel specification has been known to determine the angular field…

Optics · Physics 2021-12-07 Byung Gyu Chae

Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and…

Optics · Physics 2025-01-17 Vicente Mico , Juanjuan Zheng , Javier Garcia , Zeev Zalevsky , Peng Gao

The numerical aperture (NA) of a lens determines its ability to focus light and its resolving capability. Having a large NA is a very desirable quality for applications requiring small light-matter interaction volumes or large angular…

Imaging depth of optical microscopy has been fundamentally limited to millimeter or sub-millimeter due to light scattering. X-ray microscopy can resolve spatial details of few microns deeply inside a sample but the contrast resolution is…

Biological Physics · Physics 2015-06-17 Wenxiang Cong , Fenglin Liu , Chao Wang , Ge Wang

Ultrafast optical imaging has enabled direct observation of femtosecond-nanosecond dynamics, yet three-dimensional (3D) dynamic measurements at high numerical aperture (NA) remain hindered by the intrinsically shallow depth of field (DoF)…

In photon upconverting core-shell nanoparticles, structure strongly dictates performance. Conventional imaging in scanning transmission electron microscopy has sufficient resolution to probe the atomic structure of these nanoparticles, but…

The super-oscillation lens (SOL) can achieve super-resolution focusing but have to trade-off with weaker hotspots and higher sidebands. We propose a single compound SOL to achieve reflective confocal imaging in principle without additional…

The space-bandwidth product (SBP) imposes a fundamental limitation in achieving high-resolution and large field-of-view image acquisitions simultaneously. High-NA objectives provide fine structural detail at the cost of reduced spatial…

Optics · Physics 2025-12-17 Neha Goswami , Mark A. Anastasio

Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record…

Optics · Physics 2021-08-26 Zengbo Wang

Two-photon (2P) microscopy is a powerful technique for deep-tissue fluorescence imaging; however, tissue scattering limits its effectiveness for depth imaging using conventional approaches. Despite typical strategies having been put forward…

Optics · Physics 2025-05-21 Lei Zhu , Bernhard Rauer , Hilton B. De Aguiar , Sylvain Gigan

Multiphoton processes of absorption photoluminescence have enabled a wide range of applications including three-dimensional microfabrication, data storage, and biological imaging. While the applications of two-photon and three-photon…

Single-pixel imaging, originally developed in light optics, facilitates fast three-dimensional sample reconstruction, as well as probing with light wavelengths undetectable by conventional multi-pixel detectors. However, the spatial…