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Enzyme-catalysed reactions involve two distinct timescales. There is a short timescale on which enzymes bind to substrate molecules to produce bound complexes, and a comparatively long timescale on which the complex is transformed into a…

Quantitative Methods · Quantitative Biology 2023-10-06 Taylor Kearney , Mark B. Flegg

Despite the exciting progress in target-specific de novo protein binder design, peptide binder design remains challenging due to the flexibility of peptide structures and the scarcity of protein-peptide complex structure data. In this…

Biomolecules · Quantitative Biology 2024-09-04 Fanhao Wang , Yuzhe Wang , Laiyi Feng , Changsheng Zhang , Luhua Lai

Predicting protein complex structures is essential for protein function analysis, protein design, and drug discovery. While AI methods like AlphaFold can predict accurate structural models for many protein complexes, reliably estimating the…

Biomolecules · Quantitative Biology 2025-05-30 Pawan Neupane , Jian Liu , Jianlin Cheng

Protein representation learning has advanced rapidly with the scale-up of sequence and structure supervision, but most models still encode proteins either as per-residue token sequences or as single global embeddings. This overlooks a…

Quantitative Methods · Quantitative Biology 2026-03-03 Robert Calef , Arthur Liang , Manolis Kellis , Marinka Zitnik

Configurational entropy is an important factor in the free energy change of many macromolecular recognition and binding processes, and has been intensively studied. Despite great progresses that have been made, the global sampling remains…

Biological Physics · Physics 2012-12-04 Wenzhao Li , Kai Wang , Suyan Tian , Pu Tian

Template based single step retrosynthesis predicts reactants by selecting and applying an explicit reaction template, making each prediction traceable to a chemical transformation rule. This is useful for synthesis planning, but template…

Machine Learning · Computer Science 2026-05-14 Mohammad Jahid Ibna Basher , Ali Khodabandeh Yalabadi , Ivan Garibay , Ozlem Ozmen Garibay

Structure-based drug design (SBDD) aims to generate 3D ligand molecules that bind to specific protein targets. Existing 3D deep generative models including diffusion models have shown great promise for SBDD. However, it is complex to…

Biomolecules · Quantitative Biology 2024-03-01 Zhilin Huang , Ling Yang , Zaixi Zhang , Xiangxin Zhou , Yu Bao , Xiawu Zheng , Yuwei Yang , Yu Wang , Wenming Yang

Current approaches to genomic sequence modeling often struggle to align the inductive biases of machine learning models with the evolutionarily-informed structure of biological systems. To this end, we formulate a novel application of…

Machine Learning · Computer Science 2025-07-30 Raiyan R. Khan , Philippe Chlenski , Itsik Pe'er

Recent advances in de novo protein binder design have enabled increasing experimental validation, yet reported in silico metrics remain difficult to interpret or compare across studies due to non-standardized evaluation protocols. We…

Quantitative Methods · Quantitative Biology 2026-05-25 Cong Liu , Milong Ren , Jiaqi Guan , Chengyue Gong , Jinyuan Sun , Xinshi Chen , Wenzhi Xiao

The design and optimization of antibodies requires an intricate balance across multiple properties. Protein inverse folding models, capable of generating diverse sequences folding into the same structure, are promising tools for maintaining…

Biologists frequently desire protein inhibitors for a variety of reasons, including use as research tools for understanding biological processes and application to societal problems in agriculture, healthcare, etc. Immunotherapy, for…

Machine Learning · Computer Science 2024-11-04 Po-Yu Liang , Jun Bai

We develop ProtComposer to generate protein structures conditioned on spatial protein layouts that are specified via a set of 3D ellipsoids capturing substructure shapes and semantics. At inference time, we condition on ellipsoids that are…

Biomolecules · Quantitative Biology 2025-03-10 Hannes Stark , Bowen Jing , Tomas Geffner , Jason Yim , Tommi Jaakkola , Arash Vahdat , Karsten Kreis

Proteins are complex biomolecules that perform a variety of crucial functions within living organisms. Designing and generating novel proteins can pave the way for many future synthetic biology applications, including drug discovery.…

Computational protein design (CPD) refers to the use of computational methods to design proteins. Traditional methods relying on energy functions and heuristic algorithms for sequence design are inefficient and do not meet the demands of…

Machine Learning · Computer Science 2024-10-30 Xiaoqi Ling , Cheng Cai , Demin Kong , Zhisheng Wei , Jing Wu , Lei Wang , Zhaohong Deng

An effective potential function is critical for protein structure prediction and folding simulation. Simplified protein models such as those requiring only $C_\alpha$ or backbone atoms are attractive because they enable efficient search of…

Biomolecules · Quantitative Biology 2007-05-23 Jinfeng Zhang , Rong Chen , Jie Liang

Development of scoring functions (SFs) used to predict protein-ligand binding energies requires high-quality 3D structures and binding assay data for training and testing their parameters. In this work, we show that one of the widely-used…

Biological Physics · Physics 2025-03-10 Yingze Wang , Kunyang Sun , Jie Li , Xingyi Guan , Oufan Zhang , Dorian Bagni , Teresa Head-Gordon

Visual grounding aims to align visual information of specific regions of images with corresponding natural language expressions. Current visual grounding methods leverage pre-trained visual and language backbones independently to obtain…

Computer Vision and Pattern Recognition · Computer Science 2024-07-09 Jiaxi Wang , Wenhui Hu , Xueyang Liu , Beihu Wu , Yuting Qiu , YingYing Cai

Proteins typically exist in complexes, interacting with other proteins or biomolecules to perform their specific biological roles. Research on single-chain protein modeling has been extensively and deeply explored, with advancements seen in…

Machine Learning · Computer Science 2025-09-09 Ruizhe Chen , Dongyu Xue , Xiangxin Zhou , Zaixiang Zheng , Xiangxiang Zeng , Quanquan Gu

A fundamental challenge in protein design is the trade-off between generating structural diversity while preserving motif biological function. Current state-of-the-art methods, such as partial diffusion in RFdiffusion, often fail to resolve…

Quantitative Methods · Quantitative Biology 2025-10-22 Kevin Michalewicz , Chen Jin , Philip Alexander Teare , Tom Diethe , Mauricio Barahona , Barbara Bravi , Asher Mullokandov