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The location, timing, and abundance of gene expression (both mRNA and proteins) within a tissue define the molecular mechanisms of cell functions. Recent technology breakthroughs in spatial molecular profiling, including imaging-based…

Applications · Statistics 2020-12-10 Qiwei Li , Minzhe Zhang , Yang Xie , Guanghua Xiao

Spatial transcriptomics (ST) provides essential spatial context by mapping gene expression within tissue, enabling detailed study of cellular heterogeneity and tissue organization. However, aligning ST data with histology images poses…

Spatial Transcriptomics is a groundbreaking technology that integrates histology images with spatially resolved gene expression profiles. Among the various Spatial Transcriptomics techniques available, Visium has emerged as the most widely…

Computer Vision and Pattern Recognition · Computer Science 2025-09-05 Daniela Ruiz , Paula Cárdenas , Leonardo Manrique , Daniela Vega , Gabriel M. Mejia , Pablo Arbeláez

Spatial transcriptomics methods capture cellular measurements such as gene expression and cell types at specific locations in a cell, helping provide a localized picture of tissue health. Traditional visualization techniques superimpose the…

Quantitative Methods · Quantitative Biology 2024-10-16 Siyuan Zhao , G. Elisabeta Marai

The advent of next-generation sequencing-based spatially resolved transcriptomics (SRT) techniques has reshaped genomic studies by enabling high-throughput gene expression profiling while preserving spatial and morphological context.…

Applications · Statistics 2025-02-20 Bencong Zhu , Alberto Cassese , Marina Vannucci , Michele Guindani , Qiwei Li

Several modern genomic technologies, such as DNA-Methylation arrays, measure spatially registered probes that number in the hundreds of thousands across multiplechromosomes. The measured probes are by themselves less interesting…

Applications · Statistics 2016-11-16 John Nagorski , Genevera I. Allen

Spatial transcriptomics studies are becoming increasingly large and commonplace, necessitating simultaneous analysis of a large number of spatially resolved variables. Correspondingly, a diverse range of methodologies have been proposed to…

Quantitative Methods · Quantitative Biology 2025-09-09 James Boyle , Gregory Hamm , Eleanor Williams , Robin JG Hartman , Magnus Soderburg , Ian Henry , Michael Casey

Identifying disease-indicative genes is critical for deciphering disease mechanisms and has attracted significant interest in biomedical research. Spatial transcriptomics offers unprecedented insights for the detection of disease-specific…

Methodology · Statistics 2024-09-05 Qicheng Zhao , Qihuang Zhang

Spatial Transcriptomics (ST) profiles thousands of gene expression values at discrete spots with precise coordinates on tissue sections, preserving spatial context essential for clinical and pathological studies. With rising sequencing…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Yishun Zhu , Jiaxin Qi , Jian Wang , Yuhua Zheng , Jianqiang Huang

The rapid development of spatial transcriptomics(ST) enables the measurement of gene expression at spatial resolution, making it possible to simultaneously profile the gene expression, spatial locations of spots, and the matched…

Artificial Intelligence · Computer Science 2024-06-19 Changxi Chi , Hang Shi , Qi Zhu , Daoqiang Zhang , Wei Shao

Recent advancements in Spatial Transcriptomics (ST) technology have facilitated detailed gene expression analysis within tissue contexts. However, the high costs and methodological limitations of ST necessitate a more robust predictive…

Computer Vision and Pattern Recognition · Computer Science 2024-04-26 Youngmin Chung , Ji Hun Ha , Kyeong Chan Im , Joo Sang Lee

Spatial transcriptomics (ST) provides crucial insights into tissue micro-environments, but is limited to its high cost and complexity. As an alternative, predicting gene expression from pathology whole slide images (WSI) is gaining…

Computer Vision and Pattern Recognition · Computer Science 2025-07-01 Mingcheng Qu , Yuncong Wu , Donglin Di , Yue Gao , Tonghua Su , Yang Song , Lei Fan

Understanding how cellular morphology, gene expression, and spatial context jointly shape tissue function is a central challenge in biology. Image-based spatial transcriptomics technologies now provide high-resolution measurements of cell…

Quantitative Methods · Quantitative Biology 2026-02-17 Zhenglun Kong , Mufan Qiu , John Boesen , Xiang Lin , Sukwon Yun , Tianlong Chen , Manolis Kellis , Marinka Zitnik

In the analysis of spatially resolved transcriptomics data, detecting spatially variable genes (SVGs) is crucial. Numerous computational methods exist, but varying SVG definitions and methodologies lead to incomparable results. We review 33…

Quantitative Methods · Quantitative Biology 2024-10-04 Guanao Yan , Shuo Harper Hua , Jingyi Jessica Li

Spatial transcriptomics is an emerging technology that aligns histopathology images with spatially resolved gene expression profiling. It holds the potential for understanding many diseases but faces significant bottlenecks such as…

Computer Vision and Pattern Recognition · Computer Science 2024-01-12 Gabriel Mejia , Paula Cárdenas , Daniela Ruiz , Angela Castillo , Pablo Arbeláez

Spatial transcriptomics (ST) measures gene expression at a set of spatial locations in a tissue. Communities of nearby cells that express similar genes form \textit{spatial domains}. Specialized ST clustering algorithms have been developed…

Quantitative Methods · Quantitative Biology 2025-11-12 Perry Beamer , Zixuan Cang

Understanding sub-cellular protein localisation is an essential component to analyse context specific protein function. Recent advances in quantitative mass-spectrometry (MS) have led to high resolution mapping of thousands of proteins to…

Applications · Statistics 2019-03-12 Oliver M. Crook , Kathryn S. Lilley , Laurent Gatto , Paul D. W. Kirk

Spatial domain identification requires jointly modeling molecular signatures and physical coordinates, yet current tools frequently over-smooth biological boundaries, require user-specified cluster numbers, and lack principled multimodal…

Applications · Statistics 2026-05-18 Xin Li , Xiaofei Dong , Zhenke Duan , Lulu Shang , Xiao Wang , Xinyuan Song , Hanwen Ning , Guanyu Hu

Spatial transcriptomic (ST) clustering employs spatial and transcription information to group spots spatially coherent and transcriptionally similar together into the same spatial domain. Graph convolution network (GCN) and graph attention…

Quantitative Methods · Quantitative Biology 2023-10-24 Chen Zhang , Junhui Gao , Lingxin Kong , Guangshuo cao , Xiangyu Guo , Wei Liu

The use of covariance kernels is ubiquitous in the field of spatial statistics. Kernels allow data to be mapped into high-dimensional feature spaces and can thus extend simple linear additive methods to nonlinear methods with higher order…

Machine Learning · Statistics 2017-11-16 Jean-Francois Ton , Seth Flaxman , Dino Sejdinovic , Samir Bhatt