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Related papers: Quantification of dual-state 5-ALA-induced PpIX fl…

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Reliable in-vitro models are used for optoelectronic device development such as fluorescence detection devices for fluorescence-guided surgery of gliomas. A common approach is based on inducing gliomas in animal models. This is followed by…

Biological Physics · Physics 2025-08-06 John Raschke , Jean Pierre Ndabakuranye , Bobbi Fleiss , Arman Ahnood

Fluorescence-guided surgery has emerged as a vital tool for tumour resection procedures. As well as intraoperative tumour visualisation, 5-ALA-induced PpIX provides an avenue for quantitative tumour identification based on ratiometric…

Conventional 2D fluorescence imaging in glioma surgery cannot separate intrinsic fluorophore strength from attenuation with depth, creating depth-intensity ambiguity that can compromise assessment of residual tumour and fluorescence based…

Medical Physics · Physics 2026-04-07 Yucheng Bian , Sebastien Ourselin , Yijing Xie

The initial detection and identification of suspicious lesions and the precise delineation of tumour mar-gins are essential for successful tumour resection, with progression-free survival linked to rates of complete resection. However,…

Complete resection of malignant gliomas is hampered by the difficulty in distinguishing tumor cells at the infiltration zone. Fluorescence guidance with 5-ALA assists in reaching this goal. Using hyperspectral imaging, previous work…

In this study, a radiomics approach was extended to optical fluorescence molecular imaging data for tissue classification, termed 'optomics'. Fluorescence molecular imaging is emerging for precise surgical guidance during head and neck…

Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in…

This research paper presents a novel approach to the prediction of hypoxia in brain tumors, using multi-parametric Magnetic Resonance Imaging (MRI). Hypoxia, a condition characterized by low oxygen levels, is a common feature of malignant…

Image and Video Processing · Electrical Eng. & Systems 2024-01-26 Daniele Perlo , Georgia Kanli , Selma Boudissa , Olivier Keunen

Intraoperative optical imaging is essential for surgical precision and patient safety, but current systems present anatomical and fluorescence information separately, causing delays and increasing cognitive load. A unified system for…

Medical Physics · Physics 2025-09-22 Kyriakos Pentarakis , George Kakavelakis , Constantinos Petridis , George Themelis

In computational phase imaging with a microscope equipped with an array of light emitting diodes as illumination unit, conventional Fourier ptychographic microscopy achieves high resolution and wide-field reconstructions but is constrained…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Significance: Optical imaging of blood oxygenation (sO$_2$) can be achieved based on the differential absorption spectra of oxy- and deoxy-haemoglobin. A key challenge in realising clinical validation of the sO$_2$ biomarkers is the absence…

Motivation: Flow Injection Analysis coupled to High-Resolution Mass Spectrometry (FIA-HRMS) is a promising approach for high-throughput metabolomics. FIA-HRMS data, however, cannot be preprocessed with current software tools which rely on…

Quantitative Methods · Quantitative Biology 2017-08-15 Alexis Delabrière , Ulli Hohenester , Benoit Colsch , Christophe Junot , François Fenaille , Etienne Thévenot

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

DSC-MRI perfusion is a medical imaging technique for diagnosing and prognosing brain tumors and strokes. Its analysis relies on mathematical deconvolution, but noise or motion artifacts in a clinical environment can disrupt this process,…

Quantitative Methods · Quantitative Biology 2025-10-17 Pierre Fayolle , Alexandre Bône , Noëlie Debs , Mathieu Naudin , Pascal Bourdon , Remy Guillevin , David Helbert

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

Accurate measurement of optical absorption coefficients from photoacoustic imaging (PAI) data would enable direct mapping of molecular concentrations, providing vital clinical insight. The ill-posed nature of the problem of absorption…

Image and Video Processing · Electrical Eng. & Systems 2023-06-13 Janek Gröhl , Thomas R. Else , Lina Hacker , Ellie V. Bunce , Paul W. Sweeney , Sarah E. Bohndiek

Glioblastoma multiforme (GBM) is a fast-growing and highly invasive brain tumour, it tends to occur in adults between the ages of 45 and 70 and it accounts for 52 percent of all primary brain tumours. Usually, GBMs are detected by magnetic…

Image and Video Processing · Electrical Eng. & Systems 2020-04-21 Matteo Rucco , Lorenzo Falsetti , Giovanna Viticchi

Intra-operative identification of malignant versus benign or healthy tissue is a major challenge in fluorescence guided cancer surgery. We propose a perfusion quantification method for computer-aided interpretation of subtle differences in…

Image and Video Processing · Electrical Eng. & Systems 2020-06-26 Sergiy Zhuk , Jonathan P. Epperlein , Rahul Nair , Seshu Thirupati , Pol Mac Aonghusa , Ronan Cahill , Donal O'Shea

Pink-beam Dark-Field X-ray Microscopy (pDFXM) is a powerful emerging technique for time-resolved studies of microstructure and strain evolution in bulk crystalline materials. In this work, we systematically assess the performance of pDFXM…

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