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The forced rupture of single chemical bonds under external load is addressed. A general framework is put forward to optimally utilize the experimentally observed rupture force data for estimating the parameters of a theoretical model. As an…

Biological Physics · Physics 2009-11-13 S. Getfert , P. Reimann

Analysis of bond rupture data from single-molecule force spectroscopy experiments commonly relies on the strong assumption that the bond dissociation process is irreversible. However, with increased spatiotemporal resolution of instruments…

Biological Physics · Physics 2022-05-13 Jakob Tómas Bullerjahn , Gerhard Hummer

Dynamic force spectroscopy of single molecules is described by a model which predicts a distribution of rupture forces, the corresponding mean rupture force and variance, all amenable to experimental tests. The distribution has a pronounced…

Soft Condensed Matter · Physics 2007-05-23 O. K. Dudko , A. E. Filippov , J. Klafter , M. Urbakh

Single-molecule force spectroscopy experiments, as well as a number of other physical systems, are governed by thermally activated transitions out of a metastable state under the action of a steadily increasing external force. The main…

Soft Condensed Matter · Physics 2009-11-13 Sebastian Getfert , Mykhaylo Evstigneev , Peter Reimann

We consider reversible breaking of adhesion bonds or folding of proteins under the influence of a constant external force. We discuss the stochastic properties of the unbinding/rebinding events and analyze their mean number and their…

Soft Condensed Matter · Physics 2009-11-13 Gregor Diezemann , Andreas Janshoff

In dynamic force spectroscopy, a (bio-)molecular complex is subjected to a steadily increasing force until the chemical bond breaks. Repeating the same experiment many times results in a broad distribution of rupture forces, whose…

Biological Physics · Physics 2012-03-14 Sebastian Getfert , Peter Reimann

In recent years single molecule force spectroscopy has opened a new avenue to provide profiles of the complex energy landscape of biomolecules. In this field, quantitative analyses of the data employing sound theoretical models, have played…

Biomolecules · Quantitative Biology 2015-01-15 Changbong Hyeon , Michael Hinczewski , D. Thirumalai

Heterogeneity in biological molecules, resulting in molecule-to-molecule variations in their dynamics and function, is an emerging theme. To elucidate the consequences of heterogeneous behavior at the single molecule level, we propose an…

Biological Physics · Physics 2017-01-24 Changbong Hyeon , Michael Hinczewski , D. Thirumalai

We consider the fracture of a free-standing two-dimensional (2D) elastic-brittle network to be used as protective coating subject to constant tensile stress applied on its rim. Using a Molecular Dynamics simulation with Langevin thermostat,…

Soft Condensed Matter · Physics 2015-06-03 J. Paturej , H. Popova , A. Milchev , T. A. Vilgis

In the present paper, a fiber bundle model in (1+1)-dimensions that simulates the rupture process of a fibrous material pulled by an uniaxial force F is analyzed. In this model the load of a broken fiber is shifted in equal portions onto…

Statistical Mechanics · Physics 2016-08-31 I. L. Menezes-Sobrinho

A fiber bundle model in $(1+1)$-dimensions for the breaking of fibrous composite matrix is introduced. The model consists of $N$ parallel fibers fixed in two plates. When one of the plates is pulled in the direction parallel to the fibers,…

Condensed Matter · Physics 2009-10-22 A. T. Bernardes , J. G. Moreira

The problem of diffusive bond-dissociation in a double well potential under application of an external force is scrutinized. We compute the probability distribution of rupture forces and present a detailed discussion of the influence of…

Soft Condensed Matter · Physics 2009-11-13 Gregor Diezemann , Andreas Janshoff

Curvatures in the most probable rupture force ($f^*$) versus log-loading rate ($\log{r_f}$) observed in dynamic force spectroscopy (DFS) on biomolecular complexes are interpreted using a one-dimensional free energy profile with multiple…

Biological Physics · Physics 2015-06-04 Changbong Hyeon , D. Thirumalai

In single-molecule force spectroscopy experiments, a biomolecule is attached to a force probe via polymer linkers, and the total extension -- of molecule plus apparatus -- is monitored as a function of time. In a typical unfolding…

Chemical Physics · Physics 2019-11-06 Roberto Covino , Michael T. Woodside , Gerhard Hummer , Attila Szabo , Pilar Cossio

Thermodynamic bulk measurements of binding reactions critically rely on the validity of the law of mass action and the assumption of a dilute solution. Yet important biological systems such as allosteric ligand-receptor binding,…

Biological Physics · Physics 2017-03-31 Joan Camunas-Soler , Anna Alemany , Felix Ritort

Using the atomic force microscope based break junction approach, applicable to metal point contacts and single molecule junctions, measurements can be repeated thousands of times resulting in rich data sets characterizing the properties of…

Mesoscale and Nanoscale Physics · Physics 2017-03-08 Mark S. Hybertsen

We examined theory for force-induced unbinding on a two-dimensional free energy surface where the internal dynamics of biomolecules is coupled with the rupture process under constant tension f. We show that only if the transition state…

Soft Condensed Matter · Physics 2017-01-24 Changbong Hyeon

We consider a freely movable solid that separates a long tube into two regions, each of which is filled with a dilute gas. The gases in each region are initially prepared at the same pressure but different temperatures. Under the assumption…

Statistical Mechanics · Physics 2014-05-06 Masato Itami , Shin-ichi Sasa

We demonstrate thermally limited force spectroscopy using a probe formed by a dielectric microsphere optically trapped in water near a dielectric surface. We achieve force resolution below 1 fN in 100 s, corresponding to a 2 {\AA} rms…

Optics · Physics 2016-07-06 Lulu Liu , Simon Kheifets , Vincent Ginis , Federico Capasso

The light scattered by cold atoms induces mutual optical forces between them, which can lead to bound states. In addition to the trapping potential, this light-induced interaction generates a velocity-dependent force which damps or…

Atomic Physics · Physics 2020-07-01 Angel T. Gisbert , Nicola Piovella , Romain Bachelard
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