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Spatial omics has transformed our understanding of tissue architecture by preserving spatial context of gene expression patterns. Simultaneously, advances in imaging AI have enabled extraction of morphological features describing the…

Computer Vision and Pattern Recognition · Computer Science 2024-08-02 Eduard Chelebian , Christophe Avenel , Carolina Wählby

Spatial transcriptomics offers spatially resolved gene expression profiling within tissue sections, but its cost and limited throughput hinder large-scale deployment. To extend this capability to routine practice, recent computational…

Machine Learning · Computer Science 2026-05-07 Keunho Byeon , Jin Tae Kwak

Spatial transcriptomics (ST) enables spatially resolved gene profiling but remains expensive and low-throughput, limiting large-cohort studies and routine clinical use. Predicting spatial gene expression from routine hematoxylin and eosin…

Computer Vision and Pattern Recognition · Computer Science 2026-04-24 Changfan Wang , Xinran Wang , Donghai Liu , Fei Su , Lulu Sun , Zhicheng Zhao , Zhu Meng

Spatial transcriptomics (ST) provides high-resolution pathological images and whole-transcriptomic expression profiles at individual spots across whole-slide scales. This setting makes it an ideal data source to develop multimodal…

Computer Vision and Pattern Recognition · Computer Science 2024-11-27 Yuxiang Lin , Ling Luo , Ying Chen , Xushi Zhang , Zihui Wang , Wenxian Yang , Mengsha Tong , Rongshan Yu

Spatial transcriptomics (ST) has revolutionised transcriptomics analysis by preserving tissue architecture, allowing researchers to study gene expression in its native spatial context. However, despite its potential, ST still faces…

Quantitative Methods · Quantitative Biology 2025-05-19 Anthony Baptista , Rosamond Nuamah , Ciro Chiappini , Anita Grigoriadis

Pathology foundation models learn morphological representations through self-supervised pretraining on large-scale whole-slide images, yet they do not explicitly capture the underlying molecular state of the tissue. Spatial transcriptomics…

Computer Vision and Pattern Recognition · Computer Science 2026-03-10 Minsoo Lee , Jonghyun Kim , Juseung Yun , Sunwoo Yu , Jongseong Jang

Spatial transcriptomics (ST) reveals spatial heterogeneity of gene expression, yet its resolution is limited by current platforms. Recent methods enhance resolution via H&E-stained histology, but three major challenges persist: (1)…

Image and Video Processing · Electrical Eng. & Systems 2025-08-12 Xuepeng Liu , Zheng Jiang , Pinan Zhu , Hanyu Liu , Chao Li

The advancement of Spatial Transcriptomics (ST) has facilitated the spatially-aware profiling of gene expressions based on histopathology images. Although ST data offers valuable insights into the micro-environment of tumors, its…

Computer Vision and Pattern Recognition · Computer Science 2024-01-25 Hongyi Wang , Xiuju Du , Jing Liu , Shuyi Ouyang , Yen-Wei Chen , Lanfen Lin

Spatially resolved transcriptomics is a fast-developing set of technologies that enables the measurement of localized gene expression across spatial locations in a sample. Detecting spatially varying genes is critical for analyzing such…

Applications · Statistics 2026-04-22 Pritam Dey , Rajarshi Guhaniyogi , Yang Ni , Bani K. Mallick

Development and homeostasis in multicellular systems both require exquisite control over spatial molecular pattern formation and maintenance. Advances in spatially-resolved and high-throughput molecular imaging methods such as multiplexed…

Quantitative Methods · Quantitative Biology 2023-03-30 Alex J. Lee , Robert Cahill , Reza Abbasi-Asl

Cellular identity and function are linked to both their intrinsic genomic makeup and extrinsic spatial context within the tissue microenvironment. Spatial transcriptomics (ST) offers an unprecedented opportunity to study this, providing in…

Machine Learning · Computer Science 2026-02-16 Rui Yan , Xiaohan Xing , Xun Wang , Zixia Zhou , Md Tauhidul Islam , Lei Xing

Whole slide images (WSIs) enable weakly supervised prognostic modeling via multiple instance learning (MIL). Spatial transcriptomics (ST) preserves in situ gene expression, providing a spatial molecular context that complements morphology.…

Computer Vision and Pattern Recognition · Computer Science 2026-02-16 Lihe Liu , Xiaoxi Pan , Yinyin Yuan , Lulu Shang

Spatial transcriptomics (ST) measures gene expression at fine-grained spatial resolution, offering insights into tissue molecular landscapes. Previous methods for spatial gene expression prediction typically crop spots of interest from…

Computer Vision and Pattern Recognition · Computer Science 2025-11-26 Ruikun Zhang , Yan Yang , Liyuan Pan

Spatial Transcriptomics (ST) is a method that captures gene expression profiles aligned with spatial coordinates. The discrete spatial distribution and the super-high dimensional sequencing results make ST data challenging to be modeled…

Machine Learning · Computer Science 2025-05-08 Qingtian Zhu , Yumin Zheng , Yuling Sang , Yifan Zhan , Ziyan Zhu , Jun Ding , Yinqiang Zheng

Spatial transcriptomics (ST) measures gene expression at a set of spatial locations in a tissue. Communities of nearby cells that express similar genes form \textit{spatial domains}. Specialized ST clustering algorithms have been developed…

Quantitative Methods · Quantitative Biology 2025-11-12 Perry Beamer , Zixuan Cang

Spatial transcriptomics enables genome-wide expression analysis within native tissue context, yet identifying spatial domains remains challenging due to complex gene-spatial interactions. Existing methods typically process spatial and…

Machine Learning · Computer Science 2025-12-19 Jianping Mei , Siqi Ai , Ye Yuan

"Is it possible to predict expression levels of different genes at a given spatial location in the routine histology image of a tumor section by modeling its stain absorption characteristics?" In this work, we propose a "stain-aware"…

Image and Video Processing · Electrical Eng. & Systems 2021-08-27 Muhammad Dawood , Kim Branson , Nasir M. Rajpoot , Fayyaz ul Amir Afsar Minhas

Single-cell spatial transcriptomics (ST) offers a unique approach to measuring gene expression profiles and spatial cell locations simultaneously. However, most existing ST methods assume that cells in closer spatial proximity exhibit more…

Genomics · Quantitative Biology 2025-06-10 Xiongtao Xiao , Xiaofeng Chen , Feiyan Jiang , Songming Zhang , Wenming Cao , Cheng Tan , Zhangyang Gao , Zhongshan Li

Recent advances in multi-modal AI have demonstrated promising potential for generating the currently expensive spatial transcriptomics (ST) data directly from routine histology images, offering a means to reduce the high cost and…

Computer Vision and Pattern Recognition · Computer Science 2025-08-21 Junchao Zhu , Ruining Deng , Junlin Guo , Tianyuan Yao , Juming Xiong , Chongyu Qu , Mengmeng Yin , Yu Wang , Shilin Zhao , Haichun Yang , Daguang Xu , Yucheng Tang , Yuankai Huo

Spatially resolved transcriptomics represents a significant advancement in single-cell analysis by offering both gene expression data and their corresponding physical locations. However, this high degree of spatial resolution entails a…

Genomics · Quantitative Biology 2024-03-19 Xiaoyu Li , Wenwen Min , Shunfang Wang , Changmiao Wang , Taosheng Xu