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Microscopy has been key to tremendous advances in science, technology, and medicine, revealing structure and dynamics across time and length scales. However, combining high spatial and temporal resolution in a non-invasive, label-free…

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

Fourier ptychographic microscopy (FPM) is a powerful computational imaging modality that achieves high space-bandwidth product imaging for biomedical samples. However, its adoption is limited by slow data acquisition due to the need for…

Optics · Physics 2025-09-09 Shi Zhao , Haowen Zhou , Changhuei Yang

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Optical super-resolution microscopy is a key technology for structural biology that offers high imaging contrast and live-cell compatibility. Minimal (fluorescence) photons flux microscopy, or MINFLUX, is an emerging super-resolution…

Optics · Physics 2025-08-29 Lukas Scheiderer , Zach Marin , Jonas Ries

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

Ultrasound vascular imaging is limited by acoustic diffraction, restricting visualization of microvessels essential for understanding organ function and disease. Label-free super-resolution methods exploiting endogenous red blood cells have…

Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…

Optics · Physics 2025-02-20 Wei Wang , Zengxin Huang , Yilin Wang , Hangfeng Li , Pakorn Kanchanawong

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

Optics · Physics 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens

We report on the recently emerging (Laser) Light Sheet based Fluorescence Microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects non-destructively in high-resolution through virtual…

Instrumentation and Detectors · Physics 2011-06-17 Jan A. N. Buytaert , Emilie Descamps , Dominique Adriaens , Joris J. J. Dirckx

This paper reported a bespoke adder-based deep learning network for time-domain fluorescence lifetime imaging (FLIM). By leveraging the l1-norm extraction method, we propose a 1-D Fluorescence Lifetime AdderNet (FLAN) without…

Signal Processing · Electrical Eng. & Systems 2022-09-13 Zhenya Zang , Dong Xiao , Quan Wang , Ziao Jiao , Chen Yu , David Day-Uei Li

Single-molecule detection enables direct observation of individual biomolecular events, providing mechanistic insights into biological processes and offering a powerful tool for disease diagnostics. However, the fundamental scale mismatch…

Optics · Physics 2025-10-14 Guifeng Li , Chaoyang Gong

Single fiber imaging technology offers unique insights for research and inspection in difficult to reach and narrow spaces. In particular, ultra-compact multimode fiber (MMF) imaging, has received increasing interest over the past decade.…

Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in…

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Lukas Harris , Micah Roschelle , Jack Bartley , Mekhail Anwar

Lensless illumination single-pixel imaging with a multicore fiber (MCF) is a computational imaging technique that enables potential endoscopic observations of biological samples at cellular scale. In this work, we show that this technique…

Image and Video Processing · Electrical Eng. & Systems 2024-03-07 Olivier Leblanc , Mathias Hofer , Siddharth Sivankutty , Hervé Rigneault , Laurent Jacques

FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…

Quantitative Methods · Quantitative Biology 2017-01-09 Bryan Kaye , Peter J. Foster , Tae Yeon Yoo , Daniel J. Needleman

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

The lensless endoscope is a promising device designed to image tissues in vivo at the cellular scale. The traditional acquisition setup consists in raster scanning during which the focused light beam from the optical fiber illuminates…

Computer Vision and Pattern Recognition · Computer Science 2018-10-31 Stéphanie Guérit , Siddharth Sivankutty , Camille Scotté , John Alto Lee , Hervé Rigneault , Laurent Jacques

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

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