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Cryo-electron microscopy is a revolutionary technique that can provide 3D density maps at near-atomic resolution. However, map validation is still an open issue in the field. Despite several efforts from the community, it is possible to…

Biological Physics · Physics 2019-08-06 Sebastian Ortiz , Luka Stanisic , Boris A Rodriguez , Markus Rampp , Gerhard Hummer , Pilar Cossio

Differentiating signals from the background in micrographs is a critical initial step for cryogenic electron microscopy (cryo-EM), yet it remains laborious due to low signal-to-noise ratio (SNR), the presence of contaminants and densely…

Image and Video Processing · Electrical Eng. & Systems 2025-09-30 Szu-Chi Chung , Po-Cheng Chou

Three-dimensional reconstruction of atomic structure, known as atomic electron tomography (AET), has found increasing applications in materials science. The AET has been limited to very small nanoparticles due to the challenges of obtaining…

Materials Science · Physics 2024-01-24 Liangze Mao , Jizhe Cui , Rong Yu

Enhancing cryogenic electron microscopy (cryo-EM) 3D density maps at intermediate resolution (4-8 {\AA}) is crucial in protein structure determination. Recent advances in deep learning have led to the development of automated approaches for…

Computer Vision and Pattern Recognition · Computer Science 2025-05-16 Chenwei Zhang , Khanh Dao Duc

The third-generation of gravitational wave observatories, such as the Einstein Telescope (ET) and Cosmic Explorer (CE), aim for an improvement in sensitivity of at least a factor of ten over a wide frequency range compared to the current…

Instrumentation and Methods for Astrophysics · Physics 2022-10-05 A. Utina , A. Amato , J. Arends , C. Arina , M. de Baar , M. Baars , P. Baer , N. van Bakel , W. Beaumont , A. Bertolini , M. van Beuzekom , S. Biersteker , A. Binetti , H. J. M. ter Brake , G. Bruno , J. Bryant , H. J. Bulten , L. Busch , P. Cebeci , C. Collette , S. Cooper , R. Cornelissen , P. Cuijpers , M. van Dael , S. Danilishin , D. Dixit , S. van Doesburg , M. Doets , R. Elsinga , V. Erends , J. van Erps , A. Freise , H. Frenaij , R. Garcia , M. Giesberts , S. Grohmann , H. Van Haevermaet , S. Heijnen , J. V. van Heijningen , E. Hennes , J. -S. Hennig , M. Hennig , T. Hertog , S. Hild , H. -D. Hoffmann , G. Hoft , M. Hopman , D. Hoyland , G. A. Iandolo , C. Ietswaard , R. Jamshidi , P. Jansweijer , A. Jones , P. Jones , N. Knust , G. Koekoek , X. Koroveshi , T. Kortekaas , A. N. Koushik , M. Kraan , M. van de Kraats , S. L. Kranzhoff , P. Kuijer , K. A. Kukkadapu , K. Lam , N. Letendre , P. Li , R. Limburg , F. Linde , J. -P. Locquet , P. Loosen , H. Lueck , M. Martınez , A. Masserot , F. Meylahn , M. Molenaar , C. Mow-Lowry , J. Mundet , B. Munneke , L. van Nieuwland , E. Pacaud , D. Pascucci , S. Petit , Z. Van Ranst , G. Raskin , P. M. Recaman , N. van Remortel , L. Rolland , L. de Roo , E. Roose , J. C. Rosier , D. Ryckbosch , K. Schouteden , A. Sevrin , A. Sider , A. Singha , V. Spagnuolo , A. Stahl , J. Steinlechner , S. Steinlechner , B. Swinkels , N. Szilasi , M. Tacca , H. Thienpont , A. Vecchio , H. Verkooijen , C. H. Vermeer , M. Vervaeke , G. Visser , R. Walet , P. Werneke , C. Westhofen , B. Willke , A. Xhahi , T. Zhang

We introduce a framework for recovering an image from its rotationally and translationally invariant features based on autocorrelation analysis. This work is an instance of the multi-target detection statistical model, which is mainly used…

Image and Video Processing · Electrical Eng. & Systems 2022-03-03 Nicholas F. Marshall , Ti-Yen Lan , Tamir Bendory , Amit Singer

Resolving the structural variability of proteins is often key to understanding the structure-function relationship of those macromolecular machines. Single particle analysis using Cryogenic electron microscopy (CryoEM), combined with…

Quantitative Methods · Quantitative Biology 2022-11-22 Muyuan Chen , Bogdan Toader , Roy Lederman

Operando/in-situ liquid cell transmission electron microscopy (LCTEM) allows for real time imaging of dynamic nanoscale liquid-based processes. However, due to the thick liquid cell of traditional LCTEM holders and thus scattering of the…

Motivation: Structural heterogeneity in single-particle cryo-electron microscopy (cryo-EM) data represents a major challenge for high-resolution structure determination. Unsupervised classification may serve as the first step in the…

Data Analysis, Statistics and Probability · Physics 2017-11-01 Jiayi Wu , Yong-Bei Ma , Charles Congdon , Bevin Brett , Shuobing Chen , Qi Ouyang , Youdong Mao

Cryogenic electron microscopy (cryo-EM) is an invaluable technique for determining high-resolution three-dimensional structures of biological macromolecules using transmission particle images. The inherent symmetry in these macromolecules…

Optimization and Control · Mathematics 2024-11-06 Qi Zhang , Chenglong Bao , Hai Lin , Mingxu Hu

Cryo-electron microscopy (Cryo-EM) enables high-resolution imaging of biomolecules, but structural heterogeneity remains a major challenge in 3D reconstruction. Traditional methods assume a discrete set of conformations, limiting their…

Machine Learning · Statistics 2025-09-09 Diego Sanchez Espinosa , Erik H Thiede , Yunan Yang

The understanding of protein structure, folding, and interaction with other proteins remains one of the grand challenges of modern biology. Tremendous progress has been made thanks to X-ray- or electron-based techniques that have provided…

Accurate characterization of carbon nanotube morphologies in electron microscopy images is vital for exposure assessment and toxicological studies, yet current workflows rely on slow, subjective manual segmentation. This work presents a…

Computer Vision and Pattern Recognition · Computer Science 2026-02-06 Sanjay Pradeep , Chen Wang , Matthew M. Dahm , Jeff D. Eldredge , Candace S. J. Tsai

Consistency Training (CT) has recently emerged as a strong alternative to diffusion models for image generation. However, non-distillation CT often suffers from high variance and instability, motivating ongoing research into its training…

Machine Learning · Computer Science 2025-06-05 Gianluigi Silvestri , Luca Ambrogioni , Chieh-Hsin Lai , Yuhta Takida , Yuki Mitsufuji

Cryo-electron tomography (cryo-ET) enables high resolution, three-dimensional reconstruction of biological structures, including membranes and membrane proteins. Identification of regions of interest (ROIs) is central to scientific imaging,…

Computer Vision and Pattern Recognition · Computer Science 2026-02-25 Xingyi Cheng , Julien Maufront , Aurélie Di Cicco , Daniël M. Pelt , Manuela Dezi , Daniel Lévy

Understanding protein flexibility and its dynamic interactions with other molecules is essential for studying protein function. Although cryogenic electron microscopy(cryo-EM) provides an opportunity to observe macromolecular dynamics…

Computer Vision and Pattern Recognition · Computer Science 2026-02-03 Bintao He , Yiran Cheng , Hongjia Li , Xiang Gao , Xin Gao , Fa Zhang , Renmin Han

Atomic-resolution cryogenic scanning transmission electron microscopy (cryo-STEM) has provided a path to probing the microscopic nature of select low-temperature phases in quantum materials. Expanding cryo-STEM techniques to broadly tunable…

Applied Physics · Physics 2020-11-05 Berit H. Goodge , Elisabeth Bianco , Henny W. Zandbergen Lena F. Kourkoutis

A new iterative image reconstruction algorithm for electrical capacitance tomography (ECT) is proposed that is based on iterative soft thresholding of a total variation penalty and adaptive reweighted compressive sensing. This algorithm…

Computer Vision and Pattern Recognition · Computer Science 2016-11-22 Kezhi Li , Daniel Holland

Cellular electron cryo-tomography enables the 3D visualization of cellular organization in the near-native state and at submolecular resolution. However, the contents of cellular tomograms are often complex, making it difficult to…

Quantitative Methods · Quantitative Biology 2017-12-29 Xiangrui Zeng , Miguel Ricardo Leung , Tzviya Zeev-Ben-Mordehai , Min Xu

To handle the scarcity and heterogeneity of electroencephalography (EEG) data for Brain-Computer Interface (BCI) tasks, and to harness the power of large publicly available data sets, we propose Neuro-GPT, a foundation model consisting of…

Machine Learning · Computer Science 2024-03-05 Wenhui Cui , Woojae Jeong , Philipp Thölke , Takfarinas Medani , Karim Jerbi , Anand A. Joshi , Richard M. Leahy