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Cell counting in biomedical imaging is pivotal for various clinical applications, yet the interpretability of deep learning models in this domain remains a significant challenge. We propose a novel prototype-based method for interpretable…

Computer Vision and Pattern Recognition · Computer Science 2025-11-26 Abdurahman Ali Mohammed , Wallapak Tavanapong , Catherine Fonder , Donald S. Sakaguchi

Image-based or morphological profiling is a rapidly expanding field wherein cells are "profiled" by extracting hundreds to thousands of unbiased, quantitative features from images of cells that have been perturbed by genetic or chemical…

Quantitative Methods · Quantitative Biology 2024-11-07 Erin Weisbart , Ankur Kumar , John Arevalo , Anne E. Carpenter , Beth A. Cimini , Shantanu Singh

High-content image-based assays have fueled significant discoveries in the life sciences in the past decade (2013-2023), including novel insights into disease etiology, mechanism of action, new therapeutics, and toxicology predictions.…

Subcellular Processes · Quantitative Biology 2024-05-07 Srijit Seal , Maria-Anna Trapotsi , Ola Spjuth , Shantanu Singh , Jordi Carreras-Puigvert , Nigel Greene , Andreas Bender , Anne E. Carpenter

Generalizable neural surface reconstruction techniques have attracted great attention in recent years. However, they encounter limitations of low confidence depth distribution and inaccurate surface reasoning due to the oversimplified…

Computer Vision and Pattern Recognition · Computer Science 2023-11-03 Yixun Liang , Hao He , Ying-cong Chen

Current volumetric biomedical foundation models struggle to generalize as public 3D datasets are small and do not cover the broad diversity of medical procedures, conditions, anatomical regions, and imaging protocols. We address this by…

Computer Vision and Pattern Recognition · Computer Science 2025-03-04 Neel Dey , Benjamin Billot , Hallee E. Wong , Clinton J. Wang , Mengwei Ren , P. Ellen Grant , Adrian V. Dalca , Polina Golland

Cell painting is a popular technique for creating human-interpretable, high-contrast images of cell morphology. There are two major issues with cell paint: (1) it is labor-intensive and (2) it requires chemical fixation, making the study of…

Computer Vision and Pattern Recognition · Computer Science 2025-12-16 Alexander Peysakhovich , William Berman , Joseph Rufo , Felix Wong , Maxwell Z. Wilson

High-content screening (HCS) assays based on high-throughput microscopy techniques such as Cell Painting have enabled the interrogation of cells' morphological responses to perturbations at an unprecedented scale. The collection of such…

Machine Learning · Computer Science 2025-09-25 Mingyu Lu , Ethan Weinberger , Chanwoo Kim , Su-In Lee

Many neuroscientific applications require robust and accurate localization of neurons. It is still an unsolved problem because of the enormous variation in intensity, texture, spatial overlap, morphology and background artifacts. In…

Quantitative Methods · Quantitative Biology 2021-03-22 Tamal Batabyal , Aijaz Ahmad Naik , Daniel Weller , Jaideep Kapur

Image outpainting, which is well studied with Convolution Neural Network (CNN) based framework, has recently drawn more attention in computer vision. However, CNNs rely on inherent inductive biases to achieve effective sample learning,…

Computer Vision and Pattern Recognition · Computer Science 2022-07-13 Kai Yao , Penglei Gao , Xi Yang , Kaizhu Huang , Jie Sun , Rui Zhang

Representation learning has driven major advances in natural image analysis by enabling models to acquire high-level semantic features. In microscopy imaging, however, it remains unclear what current representation learning methods actually…

Computer Vision and Pattern Recognition · Computer Science 2026-03-17 Ivan Svatko , Maxime Sanchez , Ihab Bendidi , Gilles Cottrell , Auguste Genovesio

The visual attributes of cells, such as the nuclear morphology and chromatin openness, are critical for histopathology image analysis. By learning cell-level visual representation, we can obtain a rich mix of features that are highly…

Computer Vision and Pattern Recognition · Computer Science 2018-07-10 Bo Hu , Ye Tang , Eric I-Chao Chang , Yubo Fan , Maode Lai , Yan Xu

Cells are the fundamental unit of biological organization, and identifying them in imaging data - cell segmentation - is a critical task for various cellular imaging experiments. While deep learning methods have led to substantial progress…

Biomedical networks (or graphs) are universal descriptors for systems of interacting elements, from molecular interactions and disease co-morbidity to healthcare systems and scientific knowledge. Advances in artificial intelligence,…

Machine Learning · Computer Science 2025-02-07 Michelle M. Li , Kexin Huang , Marinka Zitnik

While deep learning has revolutionized research and applications in NLP and computer vision, this has not yet been the case for behavioral modeling and behavioral health applications. This is because the domain's datasets are smaller, have…

Machine Learning · Computer Science 2021-07-14 Mike A. Merrill , Tim Althoff

Unsupervised learning enables modeling complex images without the need for annotations. The representation learned by such models can facilitate any subsequent analysis of large image datasets. However, some generative factors that cause…

Image and Video Processing · Electrical Eng. & Systems 2020-08-27 Maxime W. Lafarge , Josien P. W. Pluim , Mitko Veta

Despite tremendous progress over the past decade, deep learning methods generally fall short of human-level systematic generalization. It has been argued that explicitly capturing the underlying structure of data should allow connectionist…

Machine Learning · Computer Science 2023-04-26 Andrea Dittadi

Background and objective: Cell-level pathological image analysis requires working with extremely small image patches (40x40 pixels), far below standard ImageNet resolutions. It remains unclear whether modern deep learning architectures and…

Computer Vision and Pattern Recognition · Computer Science 2026-03-05 Hiroki Kagiyama , Toru Nagasaka , Yukari Adachi , Takaaki Tachibana , Ryota Ito , Mitsugu Fujita , Kimihiro Yamashita , Yoshihiro Kakeji

Computational models that predict cellular phenotypic responses to chemical and genetic perturbations can accelerate drug discovery by prioritizing therapeutic hypotheses and reducing costly wet-lab iteration. However, extracting…

Computer Vision and Pattern Recognition · Computer Science 2025-09-25 Pin-Jui Huang , Yu-Hsuan Liao , SooHeon Kim , NoSeong Park , JongBae Park , DongMyung Shin

Modern methods often formulate the counting of cells from microscopic images as a regression problem and more or less rely on expensive, manually annotated training images (e.g., dot annotations indicating the centroids of cells or…

Image and Video Processing · Electrical Eng. & Systems 2021-05-18 Xin Ding , Qiong Zhang , William J. Welch

Conventional model upgrades for visual search systems require offline refresh of gallery features by feeding gallery images into new models (dubbed as "backfill"), which is time-consuming and expensive, especially in large-scale…

Computer Vision and Pattern Recognition · Computer Science 2022-03-21 Binjie Zhang , Yixiao Ge , Yantao Shen , Shupeng Su , Fanzi Wu , Chun Yuan , Xuyuan Xu , Yexin Wang , Ying Shan
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