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Fluorescence microscopy and derived techniques are continuously looking for photodetectors able to guarantee increased sensitivity, high spatial and temporal resolution and ease of integration into modern microscopy architectures. Recent…

We present a hybrid surface-enhanced Raman spectroscopy (SERS) platform based on dielectric silicon metasurfaces integrated with functionalized graphene for the selective detection of biomolecules such as prolactin and SARS-CoV-2…

The presence and density of specific types of immune cells are important to understand a patient's immune response to cancer. However, immunofluorescence staining required to identify T cell subtypes is expensive, time-consuming, and rarely…

Computer Vision and Pattern Recognition · Computer Science 2022-10-18 Georg Wölflein , In Hwa Um , David J Harrison , Ognjen Arandjelović

Histopathology, the current gold standard for cancer diagnosis, involves the manual examination of tissue samples after chemical staining, a time-consuming process requiring expert analysis. Raman spectroscopy is an alternative, stain-free…

Accurate design of labelled oligo probes for the detection of miRNA biomarkers by Surface Enhanced Raman Scattering (SERS) may improve the exploitation of the plasmonic enhancement. This work, thus, critically investigates the role of probe…

Histological examination is crucial for cancer diagnosis, including hematoxylin and eosin (H&E) staining for mapping morphology and immunohistochemistry (IHC) staining for revealing chemical information. Recently developed two-color…

Tissues and Organs · Quantitative Biology 2023-09-22 Hongli Ni , Chinmayee Prabhu Dessai , Haonan Lin , Wei Wang , Shaoxiong Chen , Yuhao Yuan , Xiaowei Ge , Jianpeng Ao , Nolan Vild , Ji-Xin Cheng

Due to its superior efficiency in utilizing annotations and addressing gigapixel-sized images, multiple instance learning (MIL) has shown great promise as a framework for whole slide image (WSI) classification in digital pathology…

Quantitative Methods · Quantitative Biology 2023-07-14 Qiehe Sun , Jiawen Li , Jin Xu , Junru Cheng , Tian Guan , Yonghong He

We have developed a multimodal photoacoustic remote sensing (PARS) microscope combined with swept source optical coherence tomography for in vivo, non-contact retinal imaging. Building on the proven strength of multiwavelength PARS imaging,…

Deep learning models can generate virtual immunohistochemistry (IHC) stains from hematoxylin and eosin (H&E) images, offering a scalable and low-cost alternative to laboratory IHC. However, reliable evaluation of image quality remains a…

Computer Vision and Pattern Recognition · Computer Science 2025-11-07 Tushar Kataria , Shikha Dubey , Mary Bronner , Jolanta Jedrzkiewicz , Ben J. Brintz , Shireen Y. Elhabian , Beatrice S. Knudsen

Integrated photonic sensors can provide large scale, flexible detection schemes. Photonic crystal slabs (PCS) offer a miniaturized platform for wideband, sensitive ultrasound detection by exploiting the photoelastic effect in water.…

Applied Physics · Physics 2021-08-17 Eric Y. Zhu , Maria Charles-Herrera , Cory Rewcastle , Raanan Gad , Li Qian , Ofer Levi

Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of…

Quantitative Methods · Quantitative Biology 2024-03-29 Yow-Ren Chang , Seong-Min Kim , Young Jong Lee

Advancements in fast electron detectors have enabled the statistically significant sampling of crystal structures on the nanometre scale by means of Scanning Electron Nanobeam Diffraction (SEND). Characterisation of structural similarity…

Materials Science · Physics 2022-07-28 Andy Bridger , William I. F. David , Thomas J. Wood , Mohsen Danaie , Keith T. Butler

Chip-based Evanescent Light Scattering (cELS) utilizes the multiple modes of a high-index contrast optical waveguide for near-field illumination of unlabeled samples, thereby repositioning the highest spatial frequencies of the sample into…

Traditional fluorescence staining is phototoxic to live cells, slow, and expensive; thus, the subcellular structure prediction (SSP) from transmitted light (TL) images is emerging as a label-free, faster, low-cost alternative. However,…

Computer Vision and Pattern Recognition · Computer Science 2024-07-04 Jintu Zheng , Yi Ding , Qizhe Liu , Yi Cao , Ying Hu , Zenan Wang

Fourier ptychographic microscopy (FPM) is a computational approach geared towards creating high-resolution and large field-of-view images without mechanical scanning. To acquire color images of histology slides, it often requires sequential…

Image and Video Processing · Electrical Eng. & Systems 2020-10-28 Ruihai Wang , Pengming Song , Shaowei Jiang , Chenggang Yan , Jiakai Zhu , Chengfei Guo , Zichao Bian , Tianbo Wang , Guoan Zheng

2D materials are considered a key element in the development of next-generation electronics (nanoelectronics) due to their extreme thickness in the nanometer range and unique physical properties. The ultrafast dynamics of photoexcited…

Mesoscale and Nanoscale Physics · Physics 2024-06-18 Yuri D Glinka

Oxides exhibiting insulator-metal transitions are promising candidates for next generation ultrafast electronic switching devices. However, critical gaps remain in understanding the onset of strain and its dynamics as these materials…

Materials Science · Physics 2026-01-09 Arthur Niedermayr , Jianyu Wu , Bertina Fisher , Ido Kaminer , Jonas Weissenrieder

Optical microscopy has long been the standard method for diagnosis in cytopathology. Whole slide scanners can image and digitize large sample areas automatically, but are slow, expensive and therefore not widely available. Clinical…

Label-free characterization of biological specimens seeks to supplement existing imaging techniques and avoid the need for contrast agents that can disturb the native state of living samples. Conventional label-free optical imaging…

Spread through air spaces (STAS) constitutes a novel invasive pattern in lung adenocarcinoma (LUAD), associated with tumor recurrence and diminished survival rates. However, large-scale STAS diagnosis in LUAD remains a labor-intensive…

Computer Vision and Pattern Recognition · Computer Science 2025-08-15 Liangrui Pan , xiaoyu Li , Guang Zhu , Guanting Li , Ruixin Wang , Jiadi Luo , Yaning Yang , Liang qingchun , Shaoliang Peng