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Biomolecular condensates are liquid- or gel-like droplets of proteins and nucleic acids formed at least in part through liquid-liquid phase separation. Condensates enable diverse functions of cells and the pathogens that infect them,…

Soft Condensed Matter · Physics 2025-05-16 Layne B. Frechette , Naren Sundararajan , Fernando Caballero , Anthony Trubiano , Michael F. Hagan

During the lifecycle of many single-stranded RNA viruses, including many human pathogens, a protein shell called the capsid spontaneously assembles around the viral genome. Understanding the mechanisms by which capsid proteins selectively…

Biomolecules · Quantitative Biology 2015-02-19 J. D. Perlmutter , M. F. Hagan

Simple RNA viruses self-assemble spontaneously and encapsulate their genome into a shell called the capsid. This process is mainly driven by the attractive electrostatic interaction between the positive charges on capsid proteins and the…

Biological Physics · Physics 2022-02-22 Sanaz Panahandeh , Siyu Li , Bogdan Dragnea , Roya Zandi

Viruses are nanoscale entities containing a nucleic acid genome encased in a protein shell called a capsid, and in some cases surrounded by a lipid bilayer membrane. This review summarizes the physics that govern the processes by which…

Biomolecules · Quantitative Biology 2015-05-20 Jason D Perlmutter , Michael F Hagan

The packaging of genetic material within a protein shell, called the capsid, marks a pivotal step in the life cycle of numerous single-stranded RNA viruses. Understanding how hundreds, or even thousands, of proteins assemble around the…

Biological Physics · Physics 2024-09-04 Siyu Li , Guillaume Tresset , Roya Zandi

Capsids of many viruses assemble around nucleic acids or other polymers. Understanding how the properties of the packaged polymer affect the assembly process could promote biomedical efforts to prevent viral assembly or nanomaterials…

Biomolecules · Quantitative Biology 2015-05-18 Aleksandr Kivenson , Michael F. Hagan

Understanding the pathways by which viral capsid proteins assemble around their genomes could identify key intermediates as potential drug targets. In this work we use computer simulations to characterize assembly over a wide range of…

Biomolecules · Quantitative Biology 2014-05-15 Jason D Perlmutter , Matthew R Perkett , Michael F Hagan

The ability of virus shells to encapsulate a wide range of functional cargoes, especially multiple cargoes - siRNAs, enzymes, and chromophores - has made them an essential tool in biotechnology for advancing drug delivery applications and…

For many viruses assembly and budding occur simultaneously during virion formation. Understanding the mechanisms underlying this process could promote biomedical efforts to block viral propagation and enable use of capsids in nanomaterials…

Subcellular Processes · Quantitative Biology 2014-03-11 Teresa Ruiz-Herrero , Michael F. Hagan

Understanding how virus capsids assemble around their nucleic acid (NA) genomes could promote efforts to block viral propagation or to reengineer capsids for gene therapy applications. We develop a coarse-grained model of capsid proteins…

Biomolecules · Quantitative Biology 2014-05-15 Jason D. Perlmutter , Cong Qiao , Michael F. Hagan

Biomolecular condensates self-assemble when proteins and nucleic acids spontaneously demix to form droplets within the crowded intracellular milieu. This simple mechanism underlies the formation of a wide variety of membraneless…

Statistical Mechanics · Physics 2021-06-30 William M. Jacobs

The formation of a viral capsid -- the highly-ordered protein shell that surrounds the genome of a virus -- is the canonical example of self-assembly. The capsids of many positive-sense RNA viruses spontaneously assemble from in vitro…

Soft Condensed Matter · Physics 2022-06-08 Rees F. Garmann , Aaron M. Goldfain , Vinothan N. Manoharan

The assembly and maturation of viruses with icosahedral capsids must be coordinated with icosahedral symmetry. The icosahedral symmetry imposes also the restrictions on the cooperative specific interactions between genomic RNA/DNA and coat…

Quantitative Methods · Quantitative Biology 2018-05-22 V. R. Chechetkin , V. V. Lobzin

We consider self-assembly of proteins into a virus capsid by the methods of molecular dynamics. The capsid corresponds either to SPMV or CCMV and is studied with and without the RNA molecule inside. The proteins are flexible and described…

Biomolecules · Quantitative Biology 2018-11-05 Karol Wolek , Marek Cieplak

We develop coarse-grained models that describe the dynamic encapsidation of functionalized nanoparticles by viral capsid proteins. We find that some forms of cooperative interactions between protein subunits and nanoparticles can…

Biomolecules · Quantitative Biology 2009-11-13 Michael F. Hagan

The coat proteins of many viruses spontaneously form icosahedral capsids around nucleic acids or other polymers. Elucidating the role of the packaged polymer in capsid formation could promote biomedical efforts to block viral replication…

Biomolecules · Quantitative Biology 2015-05-19 Oren M. Elrad , Michael F. Hagan

Single-stranded (ss) RNA viruses self-assemble spontaneously in solutions that contain the viral RNA genome molecules and the viral capsid proteins. The self-assembly of empty capsids can be understood on the basis of free energy…

Biological Physics · Physics 2021-08-21 Inbal Mizrahi , Robijn Bruinsma , Joseph Rudnick

In living cells, proteins involved in specialized biochemical functions are often spatially organized within biomolecular condensates. Increasing evidence suggests that some of these condensates, including DNA repair condensates, emerge…

Soft Condensed Matter · Physics 2026-05-15 Léa Beaulès , Judith Miné-Hattab , Pierre Illien , Vincent Dahirel

Living organisms rely on molecular networks, such as gene circuits and signaling pathways, for information processing and robust decision-making in crowded, noisy environments. Recent advances show that interacting biomolecules…

Recent studies reveal that certain viruses package a portion of their genome in a manner that mirrors the icosahedral symmetry of the protein container, or capsid. Graph theoretical constraints forbid exact realization of icosahedral…

Soft Condensed Matter · Physics 2007-05-23 Joseph Rudnick , Robijn Bruinsma
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