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Bright-field microscopy, a cost-effective solution for live-cell culture, is often the only resource available, along with standard CPUs, for many low-budget labs. The inherent challenges of bright-field images -- their noisiness, low…

Quantitative Methods · Quantitative Biology 2025-11-03 Surajit Das , Pavel Zun

Accurate segmentation of live cell images has broad applications in clinical and research contexts. Deep learning methods have been able to perform cell segmentations with high accuracy; however developing machine learning models to do this…

Image and Video Processing · Electrical Eng. & Systems 2022-12-06 Mayur Bhandary , J. Patricio Reyes , Eylul Ertay , Aman Panda

Volumetric cell segmentation in fluorescence microscopy images is important to study a wide variety of cellular processes. Applications range from the analysis of cancer cells to behavioral studies of cells in the embryonic stage. Like in…

Image and Video Processing · Electrical Eng. & Systems 2022-11-29 Royden Wagner , Karl Rohr

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

The automated analysis of microscopy images is a challenge in the context of single-cell tracking and quantification. This work has as goals the study of the performance of deep learning for segmenting microscopy images and the improvement…

Quantitative Methods · Quantitative Biology 2022-10-05 André O. Françani

Single cell segmentation is critical and challenging in live cell imaging data analysis. Traditional image processing methods and tools require time-consuming and labor-intensive efforts of manually fine-tuning parameters. Slight variations…

Quantitative Methods · Quantitative Biology 2019-04-24 Weikang Wang , David A. Taft , Yi-Jiun Chen , Jingyu Zhang , Callen T. Wallace , Min Xu , Simon C. Watkins , Jianhua Xing

Live cell microscopy sequences exhibit complex spatial structures and complicated temporal behaviour, making their analysis a challenging task. Considering cell segmentation problem, which plays a significant role in the analysis, the…

Computer Vision and Pattern Recognition · Computer Science 2019-01-08 Assaf Arbelle , Tammy Riklin Raviv

The accurate segmentation and tracking of cells in microscopy image sequences is an important task in biomedical research, e.g., for studying the development of tissues, organs or entire organisms. However, the segmentation of touching…

Computer Vision and Pattern Recognition · Computer Science 2020-12-10 Tim Scherr , Katharina Löffler , Moritz Böhland , Ralf Mikut

Cell segmentation is essential in biomedical research for analyzing cellular morphology and behavior. Deep learning methods, particularly convolutional neural networks (CNNs), have revolutionized cell segmentation by extracting intricate…

Image and Video Processing · Electrical Eng. & Systems 2024-08-27 Boyuan Peng , Jiaju Chen , P. Bilha Githinji , Ijaz Gul , Qihui Ye , Minjiang Chen , Peiwu Qin , Xingru Huang , Chenggang Yan , Dongmei Yu , Jiansong Ji , Zhenglin Chen

Cell segmentation for multi-modal microscopy images remains a challenge due to the complex textures, patterns, and cell shapes in these images. To tackle the problem, we first develop an automatic cell classification pipeline to label the…

Computer Vision and Pattern Recognition · Computer Science 2023-10-24 Wei Lou , Xinyi Yu , Chenyu Liu , Xiang Wan , Guanbin Li , Siqi Liu , Haofeng Li

Detecting and segmenting individual cells from microscopy images is critical to various life science applications. Traditional cell segmentation tools are often ill-suited for applications in brightfield microscopy due to poor contrast and…

Image and Video Processing · Electrical Eng. & Systems 2020-05-20 Rituparna Sarkar , Suvadip Mukherjee , Elisabeth Labruyère , Jean-Christophe Olivo-Marin

Living cell segmentation from bright-field light microscopy images is challenging due to the image complexity and temporal changes in the living cells. Recently developed deep learning (DL)-based methods became popular in medical and…

Quantitative Methods · Quantitative Biology 2022-07-07 Ali Ghaznavi , Renata Rychtarikova , Mohammadmehdi Saberioon , Dalibor Stys

Blood cell classification and counting are vital for the diagnosis of various blood-related diseases, such as anemia, leukemia, and thrombocytopenia. The manual process of blood cell classification and counting is time-consuming, prone to…

Image and Video Processing · Electrical Eng. & Systems 2024-05-27 Sohag Kumar Mondal , Md. Simul Hasan Talukder , Mohammad Aljaidi , Rejwan Bin Sulaiman , Md Mohiuddin Sarker Tushar , Amjad A Alsuwaylimi

The segmentation of cell nuclei in tissue images stained with the blood dye hematoxylin and eosin (H$\&$E) is essential for various clinical applications and analyses. Due to the complex characteristics of cellular morphology, a large…

Image and Video Processing · Electrical Eng. & Systems 2024-07-26 Ziwei Cui , Jingfeng Yao , Lunbin Zeng , Juan Yang , Wenyu Liu , Xinggang Wang

Over the last decade, electron microscopy has improved up to a point that generating high quality gigavoxel sized datasets only requires a few hours. Automated image analysis, particularly image segmentation, however, has not evolved at the…

Computer Vision and Pattern Recognition · Computer Science 2019-11-14 Joris Roels , Yvan Saeys

Generally, microscopy image analysis in biology relies on the segmentation of individual nuclei, using a dedicated stained image, to identify individual cells. However stained nuclei have drawbacks like the need for sample preparation, and…

Image and Video Processing · Electrical Eng. & Systems 2022-11-08 Gendarme Mathieu , Lambert Annika M. , El Debs Bachir

Cell line authentication plays a crucial role in the biomedical field, ensuring researchers work with accurately identified cells. Supervised deep learning has made remarkable strides in cell line identification by studying cell…

Computer Vision and Pattern Recognition · Computer Science 2023-06-30 Lei Tong , Adam Corrigan , Navin Rathna Kumar , Kerry Hallbrook , Jonathan Orme , Yinhai Wang , Huiyu Zhou

Investigation of cell structure is hardly imaginable without bright-field microscopy. Numerous modifications such as depth-wise scanning or videoenhancement make this method being state-of-the-art. This raises a question what maximal…

Image and Video Processing · Electrical Eng. & Systems 2020-10-29 Kirill Lonhus , Renata Rychtarikova , Ganna Platonova , Dalibor Stys

Developing clinically useful cell-level analysis tools in digital pathology remains challenging due to limitations in dataset granularity, inconsistent annotations, high computational demands, and difficulties integrating new technologies…

Computer Vision and Pattern Recognition · Computer Science 2025-12-02 Nikita Shvetsov , Thomas K. Kilvaer , Masoud Tafavvoghi , Anders Sildnes , Kajsa Møllersen , Lill-Tove Rasmussen Busund , Lars Ailo Bongo

Reliable cell segmentation and classification from biomedical images is a crucial step for both scientific research and clinical practice. A major challenge for more robust segmentation and classification methods is the large variations in…

Cell Behavior · Quantitative Biology 2017-10-31 Mo Zhang , Xiang Li , Mengjia Xu , Quanzheng Li
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