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Super-resolution microscopy overcomes the diffraction limit of conventional light microscopy in spatial resolution. By providing novel spatial or spatio-temporal information on biological processes at nanometer resolution with molecular…

Biological Physics · Physics 2021-11-10 Tianjie Yang , Yaoru Luo , Wei Ji , Ge Yang

Self-supervised learning, which is strikingly referred to as the dark matter of intelligence, is gaining more attention in biomedical applications of deep learning. In this work, we introduce a novel self-supervision objective for the…

Computer Vision and Pattern Recognition · Computer Science 2023-08-29 Royden Wagner , Carlos Fernandez Lopez , Christoph Stiller

Super-resolution optical fluctuation imaging (SOFI) provides a resolution beyond the diffraction limit by analysing stochastic fluorescence fluctuations with higher-order statistics. Using nth order spatio-temporal cross-cumulants the…

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

Deep learning methods have been successfully applied to various computer vision tasks. However, existing neural network architectures do not per se incorporate domain knowledge about the addressed problem, thus, understanding what the model…

Computer Vision and Pattern Recognition · Computer Science 2019-10-21 Iman Marivani , Evaggelia Tsiligianni , Bruno Cornelis , Nikos Deligiannis

Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…

We proposed a method to achieve superresolved optical imaging without beating the diffraction limit of light. This is achieved by magnifying the ideal optical image of the object through higher-order spatial frequency generation while…

Optics · Physics 2016-08-08 Zhixiang Li , Jianji Liu , Guoquan Zhang

Deep learning-based super-resolution models have the potential to revolutionize biomedical imaging and diagnoses by effectively tackling various challenges associated with early detection, personalized medicine, and clinical automation.…

Medical Physics · Physics 2023-06-27 Yuanzheng Ma , Xinyue Wang , Benqi Zhao , Ying Xiao , Shijie Deng , Jian Song , Xun Guan

We present a physics-informed deep learning framework to address common limitations in Confocal Laser Scanning Microscopy (CLSM), such as diffraction limited resolution, noise, and undersampling due to low laser power conditions. The…

Materials Science · Physics 2025-01-27 Zaheer Ahmad , Junaid Shabeer , Usman Saleem , Tahir Qadeer , Abdul Sami , Zahira El Khalidi , Saad Mehmood

Recent years have witnessed the tremendous development of fusing fiber-optic imaging with supervised deep learning to enable high-quality imaging of hard-to-reach areas. Nevertheless, the supervised deep learning method imposes strict…

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

Super-resolution aims at increasing image resolution by algorithmic means and has progressed over the recent years due to advances in the fields of computer vision and deep learning. Convolutional Neural Networks based on a variety of…

Image and Video Processing · Electrical Eng. & Systems 2020-08-11 M. U. Müller , N. Ekhtiari , R. M. Almeida , C. Rieke

Many microscopy applications are limited by the total amount of usable light and are consequently challenged by the resulting levels of noise in the acquired images. This problem is often addressed via (supervised) deep learning based…

Image and Video Processing · Electrical Eng. & Systems 2020-08-20 Anna S. Goncharova , Alf Honigmann , Florian Jug , Alexander Krull

Confocal microscopy is the cornerstone of cellular biology and biomedical research due to its non-destructive imaging, compatibility with live cells, sensitivity, optical sectioning, and subcellular resolution. To meet the demand for rapid…

The reconstruction of dense 3D models of face geometry and appearance from a single image is highly challenging and ill-posed. To constrain the problem, many approaches rely on strong priors, such as parametric face models learned from…

Computer Vision and Pattern Recognition · Computer Science 2018-04-02 Ayush Tewari , Michael Zollhöfer , Pablo Garrido , Florian Bernard , Hyeongwoo Kim , Patrick Pérez , Christian Theobalt

This paper demonstrates a self-supervised framework for learning voxel-wise coarse-to-fine representations tailored for dense downstream tasks. Our approach stems from the observation that existing methods for hierarchical representation…

Computer Vision and Pattern Recognition · Computer Science 2024-05-28 Eytan Kats , Jochen G. Hirsch , Mattias P. Heinrich

Unsupervised cell type identification is crucial for uncovering and characterizing heterogeneous populations in single cell omics studies. Although a range of clustering methods have been developed, most focus exclusively on intrinsic…

Artificial Intelligence · Computer Science 2025-12-12 Liang Peng , Haopeng Liu , Yixuan Ye , Cheng Liu , Wenjun Shen , Si Wu , Hau-San Wong

Following recent advancements in multimode fiber (MMF), miniaturization of imaging endoscopes has proven crucial for minimally invasive surgery in vivo. Recent progress enabled by super-resolution imaging methods with a data-driven deep…

Optics · Physics 2023-07-12 Chuncheng Zhang , Tingting Liu , Zhihua Xie , Yu Wang , Tong Liu , Qian Chen , Xiubao Sui

Super-resolution microscopy is providing unprecedented insights into biology by resolving details much below the diffraction limit. State-of-the-art Single Molecule Localization Microscopy (SMLM) techniques for super-resolution are…

Quantitative Methods · Quantitative Biology 2016-12-13 Krishna Agarwal , Radek Macháň

The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using the time-domain fingerprints from uniform nanoparticles. However, the optical diffraction limit compromises the decoding throughput and…

Optics · Physics 2022-08-09 Baolei Liu , Jiayan Liao , Yiliao Song , Jie Lu , Jiajia Zhou , Fan Wang