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Hematoxylin and Eosin (H&E)-stained images are commonly used to detect nuclear or cancerous regions in cells from images captured by a microscope. Identifying cancer cytoplasm is crucial for determining the type of cancer; hence, obtaining…

Image and Video Processing · Electrical Eng. & Systems 2025-07-08 Rebeka Sultana , Hibiki Horibe , Tomoaki Murakami , Ikuko Shimizu

Nuclei instance segmentation in hematoxylin and eosin (H&E)-stained images plays an important role in automated histological image analysis, with various applications in downstream tasks. While several machine learning and deep learning…

Computer Vision and Pattern Recognition · Computer Science 2026-01-29 Nima Torbati , Anastasia Meshcheryakova , Ramona Woitek , Sepideh Hatamikia , Diana Mechtcheriakova , Amirreza Mahbod

The distribution and appearance of nuclei are essential markers for the diagnosis and study of cancer. Despite the importance of nuclear morphology, there is a lack of large scale, accurate, publicly accessible nucleus segmentation data. To…

Image and Video Processing · Electrical Eng. & Systems 2020-12-02 Le Hou , Rajarsi Gupta , John S. Van Arnam , Yuwei Zhang , Kaustubh Sivalenka , Dimitris Samaras , Tahsin M. Kurc , Joel H. Saltz

Hematoxylin and Eosin (H&E) has been the gold standard in tissue analysis for decades, however, tissue specimens stained in different laboratories vary, often significantly, in appearance. This variation poses a challenge for both…

Image and Video Processing · Electrical Eng. & Systems 2025-06-25 Umair Khan , Jouni Härkönen , Marjukka Friman , Leena Latonen , Teijo Kuopio , Pekka Ruusuvuori

The field of histology relies heavily on antiquated tissue processing and staining techniques that limit the efficiency of pathologic diagnoses of cancer and other diseases. Current staining and advanced labeling methods are often…

Prostate cancer (PCa) is graded by pathologists by examining the architectural pattern of cancerous epithelial tissue on hematoxylin and eosin (H&E) stained slides. Given the importance of gland morphology, automatically differentiating…

We propose an unsupervised method using self-clustering convolutional adversarial autoencoders to classify prostate tissue as tumor or non-tumor without any labeled training data. The clustering method is integrated into the training of the…

Computer Vision and Pattern Recognition · Computer Science 2018-04-20 Wouter Bulten , Geert Litjens

Determining early-stage prognostic markers and stratifying patients for effective treatment are two key challenges for improving outcomes for melanoma patients. Previous studies have used tumour transcriptome data to stratify patients into…

Image and Video Processing · Electrical Eng. & Systems 2022-02-24 Lucy Godson , Navid Alemi , Jeremie Nsengimana , Graham P. Cook , Emily L. Clarke , Darren Treanor , D. Timothy Bishop , Julia Newton-Bishop , Ali Gooya

Diagnosis of breast carcinomas has so far been limited to the morphological interpretation of epithelial cells and the assessment of epithelial tissue architecture. Consequently, most of the automated systems have focused on characterizing…

Computer Vision and Pattern Recognition · Computer Science 2017-02-21 Babak Ehteshami Bejnordi , Jimmy Linz , Ben Glass , Maeve Mullooly , Gretchen L Gierach , Mark E Sherman , Nico Karssemeijer , Jeroen van der Laak , Andrew H Beck

In computational pathology, automatic nuclei instance segmentation plays an essential role in whole slide image analysis. While many computerized approaches have been proposed for this task, supervised deep learning (DL) methods have shown…

Image and Video Processing · Electrical Eng. & Systems 2023-08-04 Amirreza Mahbod , Christine Polak , Katharina Feldmann , Rumsha Khan , Katharina Gelles , Georg Dorffner , Ramona Woitek , Sepideh Hatamikia , Isabella Ellinger

Histopathology tissue analysis is considered the gold standard in cancer diagnosis and prognosis. Given the large size of these images and the increase in the number of potential cancer cases, an automated solution as an aid to…

Image and Video Processing · Electrical Eng. & Systems 2020-11-19 Mahendra Khened , Avinash Kori , Haran Rajkumar , Balaji Srinivasan , Ganapathy Krishnamurthi

Spatial transcriptomics (ST) enables the visualization of gene expression within the context of tissue morphology. This emerging discipline has the potential to serve as a foundation for developing tools to design precision medicines.…

Image and Video Processing · Electrical Eng. & Systems 2024-11-12 Shivam Kumar , Samrat Chatterjee

The classification of histopathological images is of great value in both cancer diagnosis and pathological studies. However, multiple reasons, such as variations caused by magnification factors and class imbalance, make it a challenging…

Computer Vision and Pattern Recognition · Computer Science 2022-09-01 Yibao Sun , Xingru Huang , Yaqi Wang , Huiyu Zhou , Qianni Zhang

Fine-grained classification of whole slide images (WSIs) is essential in precision oncology, enabling precise cancer diagnosis and personalized treatment strategies. The core of this task involves distinguishing subtle morphological…

Computer Vision and Pattern Recognition · Computer Science 2025-04-07 Cheng Jin , Luyang Luo , Huangjing Lin , Jun Hou , Hao Chen

Foundation models are trained on massive amounts of data to distinguish complex patterns and can be adapted to a wide range of downstream tasks with minimal computational resources. Here, we develop a foundation model for prostate cancer…

Image and Video Processing · Electrical Eng. & Systems 2024-11-25 Joona Pohjonen , Abderrahim-Oussama Batouche , Antti Rannikko , Kevin Sandeman , Andrew Erickson , Esa Pitkanen , Tuomas Mirtti

MUSE is a novel slide-free imaging technique for histological examination of tissues that can serve as an alternative to traditional histology. In order to bridge the gap between MUSE and traditional histology, we aim to convert MUSE images…

Image and Video Processing · Electrical Eng. & Systems 2020-08-20 Tanishq Abraham , Andrew Shaw , Daniel O'Connor , Austin Todd , Richard Levenson

Blood vessels (BVs) play a critical role in the Tumor Micro-Environment (TME), potentially influencing cancer progression and treatment response. However, manually quantifying BVs in Hematoxylin and Eosin (H&E) stained images is challenging…

Image and Video Processing · Electrical Eng. & Systems 2025-01-27 Jiaqi Lv , Stefan S Antonowicz , Shan E Ahmed Raza

Automated analysis of tissue sections allows a better understanding of disease biology and may reveal biomarkers that could guide prognosis or treatment selection. In digital pathology, less abundant cell types can be of biological…

Image and Video Processing · Electrical Eng. & Systems 2021-02-24 Yeman Brhane Hagos , Catherine SY Lecat , Dominic Patel , Lydia Lee , Thien-An Tran , Manuel Rodriguez- Justo , Kwee Yong , Yinyin Yuan

In this paper, we introduced a novel feature extraction approach, named exclusive autoencoder (XAE), which is a supervised version of autoencoder (AE), able to largely improve the performance of nucleus detection and classification on…

Computer Vision and Pattern Recognition · Computer Science 2018-11-29 Chao-Hui Huang , Daniel Racoceanu

In this paper, we develop a complete pipeline for stain normalization, segmentation, and classification of nuclei in hematoxylin and eosin (H&E) stained breast cancer histopathology images. In the first step, we use a CNN-based stain…

Computer Vision and Pattern Recognition · Computer Science 2018-11-12 Edwin Yuan , Junkyo Suh