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Our proposed method of random phase-free holography using virtual convergence light can obtain large reconstructed images exceeding the size of the hologram, without the assistance of random phase. The reconstructed images have low-speckle…

Biaxial anisotropy, arising from distinct optical responses along three principal directions, underlies the complex structure of many crystalline, polymeric, and biological materials. However, existing techniques such as X-ray diffraction…

Low-dose computed tomography (LDCT) plays a vital role in clinical applications by mitigating radiation risks. Nevertheless, reducing radiation doses significantly degrades image quality. Concurrently, common deep learning methods demand…

Image and Video Processing · Electrical Eng. & Systems 2024-05-28 Wenhao Zhang , Bin Huang , Shuyue Chen , Xiaoling Xu , Weiwen Wu , Qiegen Liu

We develop a novel algorithm for large-scale holographic reconstruction of 3D particle fields. Our method is based on a multiple-scattering beam propagation method (BPM) combined with sparse regularization that enables recovering dense 3D…

Optics · Physics 2021-05-24 Hao Wang , Waleed Tahir , Jiabei Zhu , Lei Tian

Transmission optical coherence tomography (OCT) enables analysis of biological specimens in vitro through detection of forward scattered light. Up to now, transmission OCT was considered as a technique that cannot directly retrieve…

Optics · Physics 2024-05-24 Wojciech Krauze , Martyna Mazur , Arkadiusz Kuś

Biophotonics is an active research area in molecular imaging, genetic diagnosis and prognosis, with direct applicability in precision medicine. However, long-standing challenges of biophotonics are well known due to low signal-to-noise…

Medical Physics · Physics 2017-10-02 Michael C Lun , Wei Zhang , Yue Zhao , Jeffrey Anker , Wenxiang Cong , Ge Wang , Changqing Li

3D printing has revolutionized the manufacturing of volumetric components and structures in many areas. Different technologies have been developed including light-induced techniques based on the photopolymerization of liquid resins. In…

X-ray microtomography is a powerful non-destructive technique allowing 3D virtual histology of resected human tissue. The achievable imaging field-of-view, is however limited by the fixed number of detector elements, enforcing the…

An organoid is a three-dimensional (3D) in vitro cell culture emulating human organs. We applied 3D dynamic optical coherence tomography (DOCT) to visualize the intratissue and intracellular activities of human induced pluripotent stem…

We demonstrate a novel approach to the reconstruction of scanning probe x-ray diffraction tomography data with anisotropic poly crystalline samples. The method involves reconstructing a voxel map containing an orientation distribution…

The task of multi-label image classification involves recognizing multiple objects within a single image. Considering both valuable semantic information contained in the labels and essential visual features presented in the image, tight…

Computer Vision and Pattern Recognition · Computer Science 2024-07-24 Shuyi Ouyang , Hongyi Wang , Ziwei Niu , Zhenjia Bai , Shiao Xie , Yingying Xu , Ruofeng Tong , Yen-Wei Chen , Lanfen Lin

Time-resolved fluorescence imaging is a key tool in biomedical applications, as it allows to non-invasively obtain functional and structural information. However, the big amount of collected data introduces challenges in both acquisition…

Deep convolutional neural networks are widely used in medical image segmentation but require many labeled images for training. Annotating three-dimensional medical images is a time-consuming and costly process. To overcome this limitation,…

Computer Vision and Pattern Recognition · Computer Science 2024-07-08 Weiyi Xie , Nathalie Willems , Nikolas Lessmann , Tom Gibbons , Daniele De Massari

We report on a novel layout providing variable zoom in digital in-line holographic microscopy (VZ-DIHM). The implementation is in virtue of an electrically tunable lens (ETL) which enables to slightly shift the illumination source axial…

Optics · Physics 2025-01-17 Martin Sanz , Maciej Trusiak , Javier Garcia , Vicente Mico

Holographic wavefront manipulation enables converting hair-thin multimode optical fibres into minimally invasive lensless imaging instruments conveying much higher information densities than conventional endoscopes. Their most prominent…

Optical coherence tomography (OCT) captures cross-sectional data and is used for the screening, monitoring, and treatment planning of retinal diseases. Technological developments to increase the speed of acquisition often results in systems…

Image and Video Processing · Electrical Eng. & Systems 2023-01-03 Timothy T. Yu , Da Ma , Jayden Cole , Myeong Jin Ju , Mirza F. Beg , Marinko V. Sarunic

We demonstrate a compact and easy-to-build computational camera for single-shot 3D imaging. Our lensless system consists solely of a diffuser placed in front of a standard image sensor. Every point within the volumetric field-of-view…

Computer Vision and Pattern Recognition · Computer Science 2017-10-06 Nick Antipa , Grace Kuo , Reinhard Heckel , Ben Mildenhall , Emrah Bostan , Ren Ng , Laura Waller

Chip-based Evanescent Light Scattering (cELS) utilizes the multiple modes of a high-index contrast optical waveguide for near-field illumination of unlabeled samples, thereby repositioning the highest spatial frequencies of the sample into…

We present a novel approach for benchmarking and validating quantitative phase tomography (QPT) systems using three-dimensional microphantoms. These microphantoms, crafted from biological and imaging data, replicate the optical and…

Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples…