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The high-throughput short-reads RNA-seq protocols often produce paired-end reads, with the middle portion of the fragments being unsequenced. We explore if the full-length fragments can be computationally reconstructed from the sequenced…

Genomics · Quantitative Biology 2023-10-06 Xiang Li , Mingfu Shao

RNAs self-interact through hydrogen-bond base-pairing between nucleotides and fold into specific, stable structures that substantially govern their biochemical behaviour. Experimental characterization of these structures remains difficult,…

Quantum Physics · Physics 2023-05-02 Tristan Zaborniak , Juan Giraldo , Hausi Müller , Hosna Jabbari , Ulrike Stege

We review the basic concepts and tools for mechanically unzipping RNA hairpins using force spectroscopy. By pulling apart the ends of an RNA molecule using optical tweezers, it is possible to measure the folding free energy at varying…

Soft Condensed Matter · Physics 2023-03-27 Paolo Rissone , Isabel Pastor , Felix Ritort

Motivated by average-case trace reconstruction and coding for portable DNA-based storage systems, we initiate the study of \emph{coded trace reconstruction}, the design and analysis of high-rate efficiently encodable codes that can be…

Information Theory · Computer Science 2019-09-11 Mahdi Cheraghchi , Ryan Gabrys , Olgica Milenkovic , João Ribeiro

The hairpin completion is an operation on formal languages which is inspired by the hairpin formation in biochemistry. Hairpin formations occur naturally within DNA-computing. It has been known that the hairpin completion of a regular…

Formal Languages and Automata Theory · Computer Science 2011-01-26 Volker Diekert , Steffen Kopecki

Though the problem of sequence-reversed protein folding is largely unexplored, one might speculate that reversed native protein sequences should be significantly more foldable than purely random heteropolymer sequences. In this article, we…

Biomolecules · Quantitative Biology 2016-06-20 Yuanzhao Zhang , Jeffrey K Weber , Ruhong Zhou

Protein folding and design are major biophysical problems, the solution of which would lead to important applications especially in medicine. Here a novel protein model capable of simultaneously provide quantitative protein design and…

Biological Physics · Physics 2015-06-22 Ivan Coluzza

Due to the redundant nature of DNA synthesis and sequencing technologies, a basic model for a DNA storage system is a multi-draw "shuffling-sampling" channel. In this model, a random number of noisy copies of each sequence is observed at…

Information Theory · Computer Science 2021-12-06 Kel Levick , Reinhard Heckel , Ilan Shomorony

We describe a strategy for constructing codes for DNA-based information storage by serial composition of weighted finite-state transducers. The resulting state machines can integrate correction of substitution errors; synchronization by…

Information Theory · Computer Science 2016-11-18 Ian Holmes

In this paper we consider the problem of RNA folding with pseudoknots. We use a graphical representation in which the secondary structures are described by planar diagrams. Pseudoknots are identified as non-planar diagrams. We analyze the…

Biomolecules · Quantitative Biology 2007-05-23 G. Vernizzi , H. Orland , A. Zee

Background: We study the sparsification of dynamic programming folding algorithms of RNA structures. Sparsification applies to the mfe-folding of RNA structures and can lead to a significant reduction of time complexity. Results: We analyze…

Combinatorics · Mathematics 2012-02-07 Fenix W. D. Huang , Christian M. Reidys

RNA folding is a kinetic process governed by the competition of a large number of structures stabilized by the transient formation of base pairs that may induce complex folding pathways and the formation of misfolded structures. Despite of…

Biological Physics · Physics 2009-03-16 M. Manosas , I. Junier , F. Ritort

Designing regulatory DNA sequences that achieve precise cell-type-specific gene expression is crucial for advancements in synthetic biology, gene therapy and precision medicine. Although transformer-based language models (LMs) can…

Machine Learning · Computer Science 2025-05-28 Xingyu Chen , Shihao Ma , Runsheng Lin , Jiecong Lin , Bo Wang

Small regulatory RNAs (sRNAs) are short non-coding RNAs in bacteria capable of post-transcriptional regulation. sRNAs have recently gained attention as tools in basic and applied sciences for example to fine-tune genetic circuits or…

Quantitative Methods · Quantitative Biology 2024-04-18 Michel Brueck , Bork A. Berghoff , Daniel Schindler

Controlling Large Language Models (LLMs) to prevent the generation of undesirable content, such as profanity and personally identifiable information (PII), has become increasingly critical. While earlier approaches relied on post-processing…

Computation and Language · Computer Science 2026-05-12 Hyundong Jin , Yo-Sub Han

The 21st century is presenting humankind with unprecedented environmental and medical challenges. The ability to design novel proteins tailored for specific purposes could transform our ability to respond timely to these issues. Recent…

Biomolecules · Quantitative Biology 2022-08-24 Noelia Ferruz , Birte Höcker

Encoding data as a set of unordered strings is receiving great attention as it captures one of the basic features of DNA storage systems. However, the challenge of constructing optimal redundancy codes for this channel remained elusive. In…

Information Theory · Computer Science 2023-08-16 Jin Sima , Netanel Raviv , Jehoshua Bruck

The tasks of designing RNAs are discrete optimization problems, and several versions of these problems are NP-hard. As an alternative to commonly used local search methods, we formulate these problems as continuous optimization and develop…

Biomolecules · Quantitative Biology 2024-03-04 Ning Dai , Wei Yu Tang , Tianshuo Zhou , David H. Mathews , Liang Huang

We study the amount of reliable information that can be stored in a DNA-based storage system with noisy sequencing, where each codeword is composed of short DNA molecules. We analyze a concatenated coding scheme, where the outer code is…

Information Theory · Computer Science 2026-05-19 Ran Tamir , Nir Weinberger , Albert Guillén i Fàbregas

Code pre-trained models (CodePTMs) have recently demonstrated a solid capacity to process various software intelligence tasks, e.g., code clone detection, code translation, and code summarization. The current mainstream method that deploys…

Software Engineering · Computer Science 2024-05-10 Qiushi Sun , Nuo Chen , Jianing Wang , Xiang Li , Ming Gao