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Registration of serial whole-slide histopathological images (WSIs) is critical for enabling direct comparison across diverse stains and for preparing paired datasets in artificial intelligence (AI) workflows such as virtual staining and…

Computer Vision and Pattern Recognition · Computer Science 2025-09-04 Zeyu Liu , Shengwei Ding

Large amounts of digitized histopathological data display a promising future for developing pathological foundation models via self-supervised learning methods. Foundation models pretrained with these methods serve as a good basis for…

Computer Vision and Pattern Recognition · Computer Science 2024-08-07 Shengyi Hua , Fang Yan , Tianle Shen , Lei Ma , Xiaofan Zhang

White blood cell (WBC) classification is fundamental for hematology applications such as infection assessment, leukemia screening, and treatment monitoring. However, real-world WBC datasets present substantial appearance variations caused…

Computer Vision and Pattern Recognition · Computer Science 2026-03-03 Luu Le , Hoang-Loc Cao , Ha-Hieu Pham , Thanh-Huy Nguyen , Ulas Bagci

Diagnosis of breast carcinomas has so far been limited to the morphological interpretation of epithelial cells and the assessment of epithelial tissue architecture. Consequently, most of the automated systems have focused on characterizing…

Computer Vision and Pattern Recognition · Computer Science 2017-02-21 Babak Ehteshami Bejnordi , Jimmy Linz , Ben Glass , Maeve Mullooly , Gretchen L Gierach , Mark E Sherman , Nico Karssemeijer , Jeroen van der Laak , Andrew H Beck

Universal, transferable whole-slide image (WSI) representations are central to computational pathology. Incorporating multiple markers (e.g., immunohistochemistry, IHC) alongside H&E enriches H&E-based features with diverse, biologically…

Computer Vision and Pattern Recognition · Computer Science 2025-12-04 Yizhi Zhang , Lei Fan , Zhulin Tao , Donglin Di , Yang Song , Sidong Liu , Cong Cong

We present an innovative method for rapidly segmenting hematoxylin and eosin (H&E)-stained tissue in whole-slide images (WSIs) that eliminates a wide range of undesirable artefacts such as pen marks and scanning artefacts. Our method…

Image and Video Processing · Electrical Eng. & Systems 2023-12-20 B. A. Schreiber , J. Denholm , F. Jaeckle , M. J. Arends , K. M. Branson , C. -B. Schönlieb , E. J. Soilleux

Training of neural networks for automated diagnosis of pigmented skin lesions is hampered by the small size and lack of diversity of available datasets of dermatoscopic images. We tackle this problem by releasing the HAM10000 ("Human…

Computer Vision and Pattern Recognition · Computer Science 2018-11-27 Philipp Tschandl , Cliff Rosendahl , Harald Kittler

Tissue segmentation is a routine preprocessing step to reduce the computational cost of whole slide image (WSI) analysis by excluding background regions. Traditional image processing techniques are commonly used for tissue segmentation, but…

Image and Video Processing · Electrical Eng. & Systems 2024-01-25 Ruben T. Lucassen , Willeke A. M. Blokx , Mitko Veta

We propose a two-step domain shift-invariant mitosis cell detection method based on Faster RCNN and a convolutional neural network (CNN). We generate various domain-shifted versions of existing histopathology images using a stain…

Computer Vision and Pattern Recognition · Computer Science 2021-09-29 Ramin Nateghi , Fattaneh Pourakpour

In histology, the presence of collagen in the extra-cellular matrix has both diagnostic and prognostic value for cancer malignancy, and can be highlighted by adding Saffron (S) to a routine Hematoxylin and Eosin (HE) staining. However,…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Guillaume Balezo , Christof A. Bertram , Cyprien Tilmant , Stéphanie Petit , Saima Ben Hadj , Rutger H. J. Fick

AI algorithms have become valuable in aiding professionals in healthcare. The increasing confidence obtained by these models is helpful in critical decision demands. In clinical dermatology, classification models can detect malignant…

Hematoxylin and Eosin stained histopathology image analysis is essential for the diagnosis and study of complicated diseases such as cancer. Existing state-of-the-art approaches demand extensive amount of supervised training data from…

Computer Vision and Pattern Recognition · Computer Science 2017-12-15 Le Hou , Ayush Agarwal , Dimitris Samaras , Tahsin M. Kurc , Rajarsi R. Gupta , Joel H. Saltz

The rapid development of digital pathology and modern deep learning has facilitated the emergence of pathology foundation models that are expected to solve general pathology problems under various disease conditions in one unified model,…

Computer Vision and Pattern Recognition · Computer Science 2025-07-10 Yutong Sun , Sichen Zhu , Peng Qiu

While multiplex immunofluorescence (mIF) imaging provides deep, spatially-resolved molecular data, integrating this information with the morphological standard of Hematoxylin & Eosin (H&E) can be very important for obtaining complementary…

Quantitative Methods · Quantitative Biology 2025-08-08 Jillur Rahman Saurav , Mohammad Sadegh Nasr , Jacob M. Luber

Current methods for diagnosing the progression of multiple types of cancer within patients rely on interpreting stained needle biopsies. This process is time-consuming and susceptible to error throughout the paraffinization, Hematoxylin and…

Image and Video Processing · Electrical Eng. & Systems 2022-04-19 Benjamin Paulson , Theodore Colwell , Natalia Bukowski , Joseph Weller , Andrew Crisler , John Cisler , Alexander Drobek , Alexander Neuwirth

Segmentation of nuclei regions from histological images is an important task for automated computer-aided analysis of histological images, particularly in the presence of impermissible color variation in the color appearance of stained…

Image and Video Processing · Electrical Eng. & Systems 2025-06-10 Suman Mahapatra , Pradipta Maji

Three-dimensional (3D) histopathology of unprocessed tissues has the potential to transform disease management by enabling volumetric characterization of tissue microarchitecture and in-vivo assessment. Back-illumination Interference…

Computer Vision and Pattern Recognition · Computer Science 2026-05-22 Anthony Song , Boyan Zhou , Mayank Golhar , Marisa Morakis , Alex Baras , Nicholas Durr

The current study detects different morphologies related to prostate pathology using deep learning models; these models were evaluated on 2,121 hematoxylin and eosin (H&E) stain histology images captured using bright field microscopy, which…

The detection of mitotic figures from different scanners/sites remains an important topic of research, owing to its potential in assisting clinicians with tumour grading. The MItosis DOmain Generalization (MIDOG) challenge aims to test the…

Computer Vision and Pattern Recognition · Computer Science 2021-09-30 Mostafa Jahanifar , Adam Shephard , Neda Zamani Tajeddin , R. M. Saad Bashir , Mohsin Bilal , Syed Ali Khurram , Fayyaz Minhas , Nasir Rajpoot

We propose a virtual staining methodology based on Generative Adversarial Networks to map histopathology images of breast cancer tissue from H&E stain to PHH3 and vice versa. We use the resulting synthetic images to build Convolutional…

Image and Video Processing · Electrical Eng. & Systems 2020-03-18 Caner Mercan , Germonda Reijnen-Mooij , David Tellez Martin , Johannes Lotz , Nick Weiss , Marcel van Gerven , Francesco Ciompi