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The enzyme turnover rate is a fundamental parameter in enzyme kinetics, reflecting the catalytic efficiency of enzymes. However, enzyme turnover rates remain scarce across most organisms due to the high cost and complexity of experimental…

Machine Learning · Computer Science 2025-09-16 Bozhen Hu , Cheng Tan , Siyuan Li , Jiangbin Zheng , Sizhe Qiu , Jun Xia , Stan Z. Li

Accurate prediction of enzyme kinetic parameters is essential for understanding catalytic mechanisms and guiding enzyme engineering.However, existing deep learning-based enzyme-substrate interaction (ESI) predictors often exhibit…

Machine Learning · Computer Science 2026-01-13 Haomin Wu , Zhiwei Nie , Hongyu Zhang , Zhixiang Ren

Predicting enzyme-substrate interactions has long been a fundamental problem in biochemistry and metabolic engineering. While existing methods could leverage databases of expert-curated enzyme-substrate pairs for models to learn from known…

Artificial Intelligence · Computer Science 2026-01-12 Tengwei Song , Long Yin , Zhen Han , Zhiqiang Xu

Biocatalysis is a promising approach to sustainably synthesize pharmaceuticals, complex natural products, and commodity chemicals at scale. However, the adoption of biocatalysis is limited by our ability to select enzymes that will catalyze…

Biomolecules · Quantitative Biology 2022-04-06 Samuel Goldman , Ria Das , Kevin K. Yang , Connor W. Coley

Spatial organization in metabolic pathways can arise from the interplay between enzymatic reaction kinetics and diffusion-driven instabilities. In this work we investigate how reversible enzyme--substrate binding influences pattern…

Molecular Networks · Quantitative Biology 2026-04-27 Faezeh Farivar

Genome-scale metabolic models (GEMs) are essential tools for systems biology and rational chassis design, but conventional top-down reconstruction depends heavily on sequence homology and often leaves unknown enzymes and metabolic dark…

Quantitative Methods · Quantitative Biology 2026-05-15 Weiyu Xiao , Jiangbin Zheng , Stan Z. Li

The concept of allostery in which macromolecules switch between two different conformations is a central theme in biological processes ranging from gene regulation to cell signaling to enzymology. Allosteric enzymes pervade metabolic…

Subcellular Processes · Quantitative Biology 2017-01-17 Tal Einav , Linas Mazutis , Rob Phillips

Enzyme mining is rapidly evolving as a data-driven strategy to identify biocatalysts with tailored functions from the vast landscape of uncharacterized proteins. The integration of machine learning into these workflows enables…

Biomolecules · Quantitative Biology 2025-07-11 Yanzi Zhang , Felix Moorhoff , Sizhe Qiu , Wenjuan Dong , David Medina-Ortiz , Jing Zhao , Mehdi D. Davari

Predicting enzyme kinetic parameters quantifies how efficiently an enzyme catalyzes a specific substrate under defined biochemical conditions. Canonical parameters such as the turnover number ($k_\text{cat}$), Michaelis constant…

Computer Vision and Pattern Recognition · Computer Science 2026-04-24 Fei Wang , Xinye Zheng , Kun Li , Yanyan Wei , Yuxin Liu , Ganpeng Hu , Tong Bao , Jingwen Yang

Enzyme design plays a crucial role in both industrial production and biology. However, this field faces challenges due to the lack of comprehensive benchmarks and the complexity of enzyme design tasks, leading to a dearth of systematic…

Biomolecules · Quantitative Biology 2024-08-21 Jiangbin Zheng , Han Zhang , Qianqing Xu , An-Ping Zeng , Stan Z. Li

Cellulases hold great promise for the production of biofuels and biochemicals. However, they are modular enzymes acting on a complex heterogeneous substrate. Because of this complexity, the computational prediction of their catalytic…

Biological Physics · Physics 2022-07-25 Kay S. Schaller , Jeppe Kari , Kim Borch , Günther H. J. Peters , Peter Westh

High-dimensional omics data contains intrinsic biomedical information that is crucial for personalised medicine. Nevertheless, it is challenging to capture them from the genome-wide data due to the large number of molecular features and…

Genomics · Quantitative Biology 2021-06-22 Xiaoyu Zhang , Yuting Xing , Kai Sun , Yike Guo

In this paper, we present an Adaptive Ensemble Learning framework that aims to boost the performance of deep neural networks by intelligently fusing features through ensemble learning techniques. The proposed framework integrates ensemble…

Artificial Intelligence · Computer Science 2023-04-07 Neelesh Mungoli

Post-translational modifications (PTMs) form a combinatorial "code" that regulates protein function, yet deciphering this code - linking modified sites to their catalytic enzymes - remains a central unsolved problem in understanding…

Computational Engineering, Finance, and Science · Computer Science 2025-10-28 Jingjie Zhang , Hanqun Cao , Zijun Gao , Yu Wang , Shaoning Li , Jun Xu , Cheng Tan , Jun Zhu , Chang-Yu Hsieh , Chunbin Gu , Pheng Ann Heng

Deep ensembles have emerged as a powerful technique for improving predictive performance and enhancing model robustness across various applications by leveraging model diversity. However, traditional deep ensemble methods are often…

Accuracy and generalization capabilities are key objectives when learning dynamical system models. To obtain such models from limited data, current works exploit prior knowledge and assumptions about the system. However, the fusion of…

Machine Learning · Statistics 2025-08-22 Björn Volkmann , Jan-Hendrik Ewering , Michael Meindl , Simon F. G. Ehlers , Thomas Seel

Predicting gene function from its DNA sequence is a fundamental challenge in biology. Many deep learning models have been proposed to embed DNA sequences and predict their enzymatic function, leveraging information in public databases…

Identifying enzymes that catalyze target biochemical reactions is a key step in computational enzyme discovery and biocatalyst design. Recent representation-learning methods formulate this problem as enzyme--reaction matching, where paired…

Biomolecules · Quantitative Biology 2026-05-26 Gengmo Zhou , Feng Yu , Wenda Wang , Zhifeng Gao , Guolin Ke , Zhewei Wei , Zhen Wang

Advancements in lithium battery technology heavily rely on the design and engineering of electrolytes. However, current schemes for molecular design and recipe optimization of electrolytes lack an effective computational-experimental closed…

Accurate electrochemical models are essential for the safe and efficient operation of lithium-ion batteries in real-world applications such as electrified vehicles and grid storage. Reduced-order models (ROM) offer a balance between…

Systems and Control · Electrical Eng. & Systems 2025-07-02 Samuel Filgueira da Silva , Mehmet Fatih Ozkan , Faissal El Idrissi , Marcello Canova
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