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Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit…

Weakly-supervised diffusion models (DMs) in anomaly segmentation, leveraging image-level labels, have attracted significant attention for their superior performance compared to unsupervised methods. It eliminates the need for pixel-level…

Computer Vision and Pattern Recognition · Computer Science 2025-01-09 Yiming Che , Fazle Rafsani , Jay Shah , Md Mahfuzur Rahman Siddiquee , Teresa Wu

We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield…

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 {\mu}m axial and 0.4 {\mu}m lateral resolution maintained over a depth of 40 {\mu}m, while preserving the advantages of Fourier domain OCM. Our method uses…

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

In this work, we demonstrated on-chip label-free imaging microscopy using real and Fourier Plane (FP) microscopic dark field images of Surface Plasmons (SP), excited on engineered 1D and 2D low aspect ratio periodic plasmonic…

Applied Physics · Physics 2018-02-13 P. Arora , A. Krishnan

In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. We present an approach for surpassing the acoustic diffraction limit by exploiting temporal fluctuations in the sample absorption…

Optics · Physics 2017-05-31 Thomas Chaigne , Bastien Arnal , Sergey Vilov , Emmanuel Bossy , Ori Katz

Imaging dynamical processes at interfaces and on the nanoscale is of great importance throughout science and technology. While light-optical imaging techniques often cannot provide the necessary spatial resolution, electron-optical…

Reconstructing high-quality images from substantially undersampled k-space data for accelerated MRI presents a challenging ill-posed inverse problem. While supervised deep learning has revolutionized this field, it relies heavily on large…

Image and Video Processing · Electrical Eng. & Systems 2025-12-22 Xinzhe Luo , Yingzhen Li , Chen Qin

A low-cost device for registration-free quantitative phase microscopy (QPM) based on the transport of intensity equation (TIE) of cells in continuous flow is presented. The method uses acoustic focusing to align cells into a single plane…

Perfusion by the microcirculation is key to the development, maintenance and pathology of tissue. Its measurement with high spatiotemporal resolution is consequently valuable but remains a challenge in deep tissue. Ultrasound Localization…

Signal Processing · Electrical Eng. & Systems 2023-04-26 Kai Riemer , Matthieu Toulemonde , Jipeng Yan , Marcelo Lerendegui , Eleanor Stride , Peter D. Weinberg , Christopher Dunsby , Meng-Xing Tang

The row-column addressed array is an emerging probe for ultrafast 3-D ultrasound imaging. It achieves this with far fewer independent electronic channels and a wider field of view than traditional 2-D matrix arrays, of the same channel…

With applications ranging from metabolomics to histopathology, quantitative phase microscopy (QPM) is a powerful label-free imaging modality. Despite significant advances in fast multiplexed imaging sensors and deep-learning-based inverse…

The vast majority of the microscopy methods currently available to study biological samples require staining prior to imaging. Nevertheless, the ability to reveal specific cell structures or organelles in a label-free manner remains…

Biological Physics · Physics 2020-10-06 R. Desapogu , G. Le Marchand , R. Smith , P. Ray , E. Gillier , S. Dutertre , M. Alouini , M. Tramier , S. Huet , J. Fade

Ultrasonography offers an inexpensive, widely-accessible and compact medical imaging solution. However, compared to other imaging modalities such as CT and MRI, ultrasound images notoriously suffer from strong speckle noise, which…

Signal Processing · Electrical Eng. & Systems 2021-12-28 Vincent van de Schaft , Ruud J. G. van Sloun

Lipid droplets (LDs), once considered mere storage depots for lipids, have gained recognition for their intricate roles in cellular processes, including metabolism, membrane trafficking, and disease states like obesity and cancer. This…

Biological Physics · Physics 2023-10-26 Hyeonwoo Kim , Seungeun Oh , Seongsoo Lee , Kwang suk Lee , YongKeun Park

Labelfree nanoscopy encompasses optical imaging with resolution in the 100 nm range using visible wavelengths. Here, we present a labelfree nanoscopy method that combines Fourier ptychography with waveguide microscopy to realize a…

Identifying and visualizing vasculature within organs and tumors has major implications in managing cardiovascular diseases and cancer. Contrast-enhanced ultrasound scans detect slow-flowing blood, facilitating non-invasive perfusion…

Medical Physics · Physics 2018-09-18 Avinoam Bar-Zion , Oren Solomon , Charles Tremblay-Darveau , Dan Adam , Yonina C. Eldar

Holography relies on the interference between a known reference and a signal of interest to reconstruct both the amplitude and phase of that signal. Commonly performed with photons and electrons, it finds numerous applications in imaging,…

Integrating femtosecond (fs) lasers to electron microscopies has enabled direct imaging of transient structures and morphologies of materials in real time and space, namely, ultrafast electron microscopy (UEM). Here we report the…