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We present an application of the well-known Mask R-CNN approach to the counting of different types of bacterial colony forming units that were cultured in Petri dishes. Our model was made available to lab technicians in a modern SPA…

Machine Learning · Computer Science 2021-03-10 Tanguy Naets , Maarten Huijsmans , Paul Smyth , Laurent Sorber , Gaël de Lannoy

Early detection and identification of pathogenic bacteria such as Escherichia coli (E. coli) is an essential task for public health. The conventional culture-based methods for bacterial colony detection usually take >24 hours to get the…

We present a computational live bacteria detection system that periodically captures coherent microscopy images of bacterial growth inside a 60 mm diameter agar-plate and analyzes these time-lapsed holograms using deep neural networks for…

Counting microbial colonies is a fundamental task in microbiology and has many applications in numerous industry branches. Despite this, current studies towards automatic microbial counting using artificial intelligence are hardly…

Computer Vision and Pattern Recognition · Computer Science 2022-09-07 Sylwia Majchrowska , Jarosław Pawłowski , Natalia Czerep , Aleksander Górecki , Jakub Kuciński , Tomasz Golan

Tuberculosis is a contagious disease which is one of the leading causes of death, globally. The general diagnosis methods for tuberculosis include microscopic examination, tuberculin skin test, culture method, enzyme linked immunosorbent…

Image and Video Processing · Electrical Eng. & Systems 2021-05-25 Dinesh Jackson Samuel , Rajesh Kanna Baskaran

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in…

Quantitative Methods · Quantitative Biology 2022-06-07 Santosh Pandey , Yunsoo Park , Ankita Ankita , Gregory J. Phillips

Microorganisms such as bacteria and fungi play essential roles in many application fields, like biotechnique, medical technique and industrial domain. Microorganism counting techniques are crucial in microorganism analysis, helping…

Image and Video Processing · Electrical Eng. & Systems 2021-09-30 Jiawei Zhang , Chen Li , Md Mamunur Rahaman , Yudong Yao , Pingli Ma , Jinghua Zhang , Xin Zhao , Tao Jiang , Marcin Grzegorzek

Aim: To evaluate a flow cytometry protocol that uses reference beads for the enumeration of live and dead bacteria present in a mixture. Methods and Results: Mixtures of live and dead Escherichia coli with live:dead concentration ratios…

Quantitative Methods · Quantitative Biology 2019-01-24 Fang Ou , Cushla McGoverin , Simon Swift , Frederique Vanholsbeeck

The spread of microbial infections is governed by the self-organization of bacteria on surfaces. Limitations of live imaging techniques make collective behaviors in clinically relevant systems challenging to quantify. Here, novel…

Biological Physics · Physics 2024-07-19 Vincent Hickl , Abid Khan , René M. Rossi , Bruno F. B. Silva , Katharina Maniura-Weber

Bacteraemia, a bloodstream infection with high morbidity and mortality rates, poses significant diagnostic challenges. Accurate diagnosis through blood cultures is resource-intensive. Developing a machine learning model to predict blood…

Machine Learning · Computer Science 2024-10-29 Kira Sam

A novel and fast semi-automatic method for segmentation, locating and counting blood cells in an image is proposed. In this method, thresholding is used to separate the nucleus from the other parts. We also use Hough transform for circles…

Image and Video Processing · Electrical Eng. & Systems 2020-04-21 Hamed Sadeghi , Shahram Shirani , David W. Capson

Counting cells in fluorescent microscopy is a tedious, time-consuming task that researchers have to accomplish to assess the effects of different experimental conditions on biological structures of interest. Although such objects are…

Computer Vision and Pattern Recognition · Computer Science 2021-03-02 R. Morelli , L. Clissa , M. Dalla , M. Luppi , L. Rinaldi , A. Zoccoli

During the development of vaccines, bacterial colony forming units (CFUs) are counted in order to quantify the yield in the fermentation process. This manual task is time-consuming and error-prone. In this work we test multiple segmentation…

Computer Vision and Pattern Recognition · Computer Science 2020-09-03 Thomas Beznik , Paul Smyth , Gaël de Lannoy , John A. Lee

The in vitro clonogenic assay is a technique to study the ability of a cell to form a colony in a culture dish. By optical imaging, dishes with stained colonies can be scanned and assessed digitally. Identification, segmentation and…

Computer Vision and Pattern Recognition · Computer Science 2022-02-15 Delmon Arous , Stefan Schrunner , Ingunn Hanson , Nina F. J. Edin , Eirik Malinen

Existing image-based pest counting methods rely on single static images and often produce inaccurate results under occlusion. To address this issue, this paper proposes an automated pest counting method in water traps through active robotic…

Robotics · Computer Science 2026-03-10 Xumin Gao , Mark Stevens , Grzegorz Cielniak

Bacterial infectious diseases are a major threat to human health. Timely and sensitive pathogenic bacteria detection is crucial in identifying the bacterial contaminations and preventing the spread of infectious diseases. Due to limitations…

A Rapid and cost-effective method for detecting bacterial cells on surfaces is critical to protect public health from various aspects, including food safety, clinical hygiene, and pharmacy quality. Herein, we first established an optical…

Quantitative Methods · Quantitative Biology 2024-03-13 Yuzhen Zhang , Zili Gao , Lili He

This paper introduces a new optimisation algorithm, called Adaptive Bacterial Colony Optimisation (ABCO), modelled after the foraging behaviour of E. coli bacteria. The algorithm follows three stages--explore, exploit and reproduce--and is…

Neural and Evolutionary Computing · Computer Science 2025-05-05 Barisi Kogam , Yevgeniya Kovalchuk , Mohamed Medhat Gaber

We developed a digital inline holography (DIH) system integrated with deep learning algorithms for real-time detection of particulate matter (PM) and bacterial contamination in peritoneal dialysis (PD) fluids. The system comprises a…

Optics · Physics 2024-11-05 Nicholas Bravo-Frank , Nicolas Mesyngier , Lei Feng , Jiarong Hong

A rapid, cost-effective and easy method that allows on-site determination of the concentration of live and dead bacterial cells using a fibre-based spectroscopic device (the optrode system) is proposed and demonstrated. Identification of…

Quantitative Methods · Quantitative Biology 2018-09-12 Fang Ou , Cushla McGoverin , Simon Swift , Frédérique Vanholsbeeck
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