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Real-time deformability cytometry (RT-DC) is an established method that quantifies features like size, shape, and stiffness for whole cell populations on a single-cell level in real time. To extract the cell stiffness, a lookup table (LUT)…

Quantitative Methods · Quantitative Biology 2022-08-29 Lucas Daniel Wittwer , Felix Reichel , Paul Müller , Jochen Guck , Sebastian Aland

A technique for determining the permeability of a phospholipid membrane on a single giant unilamellar vesicle (GUV) is described, which complements the existing methods utilizing either a planar black lipid membrane or sub-micrometre-sized…

Instrumentation and Detectors · Physics 2009-03-27 Primoz Peterlin , Gasper Jaklic , Tomaz Pisanski

This work attempts to understand the mechanism of simultaneous electrodeformation and electroporation in Giant Unilamellar Vesicles (GUVs) using a minimal analytical model. In the small deformation limit, the coupled electroporation,…

Biological Physics · Physics 2025-10-15 Rochish M. Thaokar , Rupesh Kumar , Nalinikanta Behera , Mohammad Maoyafikuddin

The deformability of vesicles critically influences their engulfment by lipid membranes, a process central to endocytosis, viral entry, drug delivery, and intercellular transport. While theoretical models have long predicted this influence,…

Soft Condensed Matter · Physics 2025-07-24 Stijn van der Ham , Alexander Brown , Halim Kusumaatmaja , Hanumantha Rao Vutukuri

The way living tissues respond to external mechanical forces is crucial in physiological processes like embryogenesis, homeostasis or tumor growth. Providing a complete description across length scales which relates the properties of…

Soft Condensed Matter · Physics 2025-11-07 Majid Layachi , Remi Merindol , Laura Casanellas

As described in the work of Mietke et al. (1) the deformation (defined as 1 - circularity [see (2)]) of a purely elastic, spherical object deformed in a real-time deformability cytometry (RT-DC) experiment can be mapped to its apparent…

Soft Condensed Matter · Physics 2017-04-04 Christoph Herold

Ultrasound-guided drug and gene delivery (usdg) enables controlled and spatially precise delivery of drugs and macromolecules, encapsulated in microbubbles (embs) and submicron gas vesicles (gvs), to target areas such as cancer tumors. It…

Soft Condensed Matter · Physics 2025-04-29 Nikolaos Ntarakas , Maša Lah , Daniel Svenšek , Tilen Potisk , Matej Praprotnik

The low elastic modulus of soft materials, combined with geometric nonlinearity and rate dependence, presents significant challenges in the characterization of their mechanical response. We introduce a novel method for measuring the…

Materials Science · Physics 2024-09-18 Jian Li , Zihao Xie , Hannah Varner , Chockalingam Senthilnathan , Tal Cohen

General-purpose Large Vision-Language Models (LVLMs), despite their massive scale, often falter in dermatology due to "diffuse attention" - the inability to disentangle subtle pathological lesions from background noise. In this paper, we…

Computer Vision and Pattern Recognition · Computer Science 2026-01-15 Lijun Liu , Linwei Chen , Zhishou Zhang , Meng Tian , Hengfu Cui , Ruiyang Li , Zhaocheng Liu , Qiang Ju , Qianxi Li , Hong-Yu Zhou

We investigated the thermodynamic relaxation of giant unilamellar vesicles (GUVs) which contained small vesicles within their interior. Quenching these vesicles from their fluid phase (T>Tm) through the phase transition in the gel state…

Biological Physics · Physics 2013-03-27 C. T. Leirer , B. Wunderlich , A. Wixforth , M. F. Schneider

A method for determining permeability of phospholipid bilayer based on the osmotic swelling of micrometer-sized giant unilamellar vesicles (GUVs) is presented as an alternative to the two established techniques, dynamic light scattering on…

Biological Physics · Physics 2012-08-16 Primoz Peterlin , Vesna Arrigler , Haim Diamant , Emir Haleva

Minimally invasive experimental methods that can measure local rate dependent mechanical properties are essential in understanding the behaviour of soft and biological materials in a wide range of applications. Needle based measurement…

Soft Condensed Matter · Physics 2020-10-20 S Chockalingam , Christine Roth , Thomas Henzel , Tal Cohen

X-ray computed tomography (XCT) has become a reliable metrology tool for measuring internal flaws and other microstructural features in engineering materials. However, tracking of material points to measure three-dimensional (3D)…

Materials Science · Physics 2023-04-13 Zifan Wang , Akshay Joshi , Angkur Jyoti Dipanka Shaikeea , Vikram Susdhir Deshpande

Tissue mechanics--stiffness, density and impedance contrast--are broadly informative biomarkers across diseases, yet routine CT, MRI, and B-mode ultrasound rarely quantify them directly. While ultrasound tomography (UT) is intrinsically…

Computer Vision and Pattern Recognition · Computer Science 2025-11-11 Zhijun Zeng , Youjia Zheng , Chang Su , Qianhang Wu , Hao Hu , Zeyuan Dong , Shan Gao , Yang Lv , Rui Tang , Ligang Cui , Zhiyong Hou , Weijun Lin , Zuoqiang Shi , Yubing Li , He Sun

This study introduces a systematic and optimised methodology for designing Linear Variable Differential Transformer (LVDT) sensors and Voice Coil (VC) actuators, tailored for high-precision applications such as gravitational wave detectors…

Instrumentation and Detectors · Physics 2026-03-10 Kumar Akhil Kukkadapu , Hans Van Haevermaet , Wim Beaumont , Nick van Remortel

Endocytosis is a key cellular process involved in the uptake of nutrients, pathogens or the diagnosis and therapy of diseases. Most studies have focused on spherical objects, whereas biologically relevant shapes can be highly anisotropic.…

Soft Condensed Matter · Physics 2023-06-07 Ali Azadbakht , Billie Meadowcroft , Thijs Varkevisser , Andela Saric , Daniela J. Kraft

Identifying, measuring and reporting lesions accurately and comprehensively from patient CT scans are important yet time-consuming procedures for physicians. Computer-aided lesion/significant-findings detection techniques are at the core of…

Computer Vision and Pattern Recognition · Computer Science 2020-09-01 Jinzheng Cai , Ke Yan , Chi-Tung Cheng , Jing Xiao , Chien-Hung Liao , Le Lu , Adam P. Harrison

The liquid-based detectors are widely used in particle and nuclear physics experiments. Due to the fixed way of constructing geometry in detector simulation such as Geant4, it is usually difficult to describe the non-uniformity of liquid in…

Instrumentation and Detectors · Physics 2021-03-11 Shu Zhang , Jing-Shu Li , Yang-Jie Su , Yu-Mei Zhang , Zi-Yuan Li , Zheng-Yun You

Vesicoureteral reflux (VUR) is traditionally assessed using subjective grading systems, which introduces variability in diagnosis. This study investigates the use of machine learning to improve diagnostic consistency by analyzing voiding…

The rheological characterisation of complex fluids is mostly performed under simple shear flow in rotational rheometers. Their modern commercial versions are extremely sensitive instruments which are able to provide very accurate…

Fluid Dynamics · Physics 2021-03-05 Ahmad Fakhari , Francisco J. Galindo-Rosales
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