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Here we report nonlinear focal modulation microscopy (NFOMM) to achieve super-resolution imaging. Abandoning the previous persistence on minimizing the size of Gaussian emission pattern by directly narrowing (e.g. Minimizing the detection…

We present the experimental reconstruction of sub-wavelength features from the far-field intensity of sparse optical objects: sparsity-based sub-wavelength imaging combined with phase-retrieval. As examples, we demonstrate the recovery of…

Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed…

The advent of single molecule optics has had a profound impact in fields ranging from biophysics to material science, photophysics, and quantum optics. However, all existing room-temperature single molecule methods have been based on…

Mesoscale and Nanoscale Physics · Physics 2010-07-22 Michele Celebrano , Philipp Kukura , Alois Renn , Vahid Sandoghdar

Monocular camera calibration is a key precondition for numerous 3D vision applications. Despite considerable advancements, existing methods often hinge on specific assumptions and struggle to generalize across varied real-world scenarios,…

Computer Vision and Pattern Recognition · Computer Science 2024-05-27 Xiankang He , Guangkai Xu , Bo Zhang , Hao Chen , Ying Cui , Dongyan Guo

The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware…

Information Theory · Computer Science 2013-07-18 Makhlad Chahid , Jerome Bobin , Hamed Shams Mousavi , Emmanuel Candes , Maxime Dahan , Vincent Studer

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

The development of X-ray Free Electron Lasers (XFELs) has opened numerous opportunities to probe atomic structure and ultrafast dynamics of various materials. Single Particle Imaging (SPI) with XFELs enables the investigation of biological…

Computer Vision and Pattern Recognition · Computer Science 2023-11-29 Zhantao Chen , Cong Wang , Mingye Gao , Chun Hong Yoon , Jana B. Thayer , Joshua J. Turner

Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light…

In this work, we propose a novel unsupervised deep learning model to address multi-focus image fusion problem. First, we train an encoder-decoder network in unsupervised manner to acquire deep feature of input images. And then we utilize…

Computer Vision and Pattern Recognition · Computer Science 2020-09-25 Boyuan Ma , Xiaojuan Ban , Haiyou Huang , Yu Zhu

Resolving sources beyond the diffraction limit is important in imaging, communications, and metrology. Current image-based methods of super-resolution require phase information (either of the source points or an added filter) and perfect…

Optics · Physics 2025-12-16 S. A. Wadood , Shaurya Aarav , Kevin Liang , Jason W Fleischer

Deep neural networks have emerged as effective tools for computational imaging including quantitative phase microscopy of transparent samples. To reconstruct phase from intensity, current approaches rely on supervised learning with training…

Image and Video Processing · Electrical Eng. & Systems 2020-01-28 Emrah Bostan , Reinhard Heckel , Michael Chen , Michael Kellman , Laura Waller

Super-resolved far-field microscopy has emerged as a powerful tool for investigating the structure of objects with resolution well below the diffraction limit of light. Nearly all super-resolution imaging techniques reported to date rely on…

The applications of present nanoscopy techniques for live cell imaging are limited by the long sampling time and low emitter density. Here we developed a new single frame wide-field nanoscopy based on ghost imaging via sparsity constraints…

High-resolution imaging of ultracold atoms typically requires custom high numerical aperture (NA) optics, as is the case for quantum gas microscopy. These high NA objectives involve many optical elements each of which contributes to loss…

Quantum Gases · Physics 2021-11-01 Emine Altuntas , Ian B. Spielman

Deep learning-based super-resolution models have the potential to revolutionize biomedical imaging and diagnoses by effectively tackling various challenges associated with early detection, personalized medicine, and clinical automation.…

Medical Physics · Physics 2023-06-27 Yuanzheng Ma , Xinyue Wang , Benqi Zhao , Ying Xiao , Shijie Deng , Jian Song , Xun Guan

We report the experimental super-resolution of pairs of point-like fluorescent sources using a modified image inversion interferometer microscope. The technique is inspired by recent developments in the application of quantum parameter…

Single-molecule fluorescence techniques are essential for investigating the molecular mechanisms in biological processes. However, achieving sub-millisecond temporal resolution to monitor fast molecular dynamics remains a significant…

Optics · Physics 2023-03-02 Sunny Tiwari , Prithu Roy , Jean-Benoît Claude , Jérôme Wenger

Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…

Optics · Physics 2025-02-20 Wei Wang , Zengxin Huang , Yilin Wang , Hangfeng Li , Pakorn Kanchanawong

Super-resolution fluorescence microscopy, with a resolution beyond the diffraction limit of light, has become an indispensable tool to directly visualize biological structures in living cells at a nanometer-scale resolution. Despite…

Computer Vision and Pattern Recognition · Computer Science 2018-09-05 Yu Li , Fan Xu , Fa Zhang , Pingyong Xu , Mingshu Zhang , Ming Fan , Lihua Li , Xin Gao , Renmin Han