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Understanding the intricate cellular environment within biological tissues is crucial for uncovering insights into complex biological functions. While single-cell RNA sequencing has significantly enhanced our understanding of cellular…

Image and Video Processing · Electrical Eng. & Systems 2024-11-06 Bingjun Li , Mostafa Karami , Masum Shah Junayed , Sheida Nabavi

Spatial transcriptomics (ST) enables transcriptome-wide profiling while preserving the spatial context of tissues, offering unprecedented opportunities to study tissue organization and cell-cell interactions in situ. Despite recent…

Computer Vision and Pattern Recognition · Computer Science 2026-01-21 Wei Wang , Quoc-Toan Ly , Chong Yu , Jun Bai

Spatial transcriptomics (ST) enables gene expression mapping within anatomical context but remains costly and low-throughput. Hematoxylin and eosin (H\&E) staining offers rich morphology yet lacks molecular resolution. We present…

Spatial Transcriptomics (ST) technologies provide biologists with rich insights into single-cell biology by preserving spatial context of cells. Building foundational models for ST can significantly enhance the analysis of vast and complex…

Genomics · Quantitative Biology 2025-07-24 Suyuan Zhao , Yizhen Luo , Ganbo Yang , Yan Zhong , Hao Zhou , Zaiqing Nie

Spatial transcriptomics (ST) enables mapping gene expression with spatial context but is severely affected by high sparsity and technical noise, which conceals true biological signals and hinders downstream analyses. To address these…

Machine Learning · Computer Science 2026-03-10 Sayeem Bin Zaman , Fahim Hafiz , Riasat Azim

Spatial Transcriptomics (ST) enables the measurement of gene expression while preserving spatial information, offering critical insights into tissue architecture and disease pathology. Recent developments have explored the use of…

Computer Vision and Pattern Recognition · Computer Science 2026-05-20 Hai Dang Nguyen , Nguyen Dang Huy Pham , The Minh Duc Nguyen , Dac Thai Nguyen , Hang Thi Nguyen , Duong M. Nguyen

Spatial transcriptomics is a modern sequencing technology that allows the measurement of the activity of thousands of genes in a tissue sample and map where the activity is occurring. This technology has enabled the study of the so-called…

Methodology · Statistics 2022-09-15 Andrea Sottosanti , Davide Risso

Cellular identity and function are linked to both their intrinsic genomic makeup and extrinsic spatial context within the tissue microenvironment. Spatial transcriptomics (ST) offers an unprecedented opportunity to study this, providing in…

Machine Learning · Computer Science 2026-02-16 Rui Yan , Xiaohan Xing , Xun Wang , Zixia Zhou , Md Tauhidul Islam , Lei Xing

Spatial transcriptomics (ST) provides spatially resolved measurements of gene expression, enabling characterization of the molecular landscape of human tissue beyond histological assessment as well as localized readouts that can be aligned…

Computer Vision and Pattern Recognition · Computer Science 2026-02-17 Konstantin Hemker , Andrew H. Song , Cristina Almagro-Pérez , Guillaume Jaume , Sophia J. Wagner , Anurag Vaidya , Nikola Simidjievski , Mateja Jamnik , Faisal Mahmood

Spatial Transcriptomics (ST) is a method that captures gene expression profiles aligned with spatial coordinates. The discrete spatial distribution and the super-high dimensional sequencing results make ST data challenging to be modeled…

Machine Learning · Computer Science 2025-05-08 Qingtian Zhu , Yumin Zheng , Yuling Sang , Yifan Zhan , Ziyan Zhu , Jun Ding , Yinqiang Zheng

Spatial transcriptomics (ST) technologies have revolutionized the study of gene expression patterns in tissues by providing multimodality data in transcriptomic, spatial, and morphological, offering opportunities for understanding tissue…

Computational Engineering, Finance, and Science · Computer Science 2024-01-17 Zelin Zang , Liangyu Li , Yongjie Xu , Chenrui Duan , Kai Wang , Yang You , Yi Sun , Stan Z. Li

Whole slide images (WSIs) enable weakly supervised prognostic modeling via multiple instance learning (MIL). Spatial transcriptomics (ST) preserves in situ gene expression, providing a spatial molecular context that complements morphology.…

Computer Vision and Pattern Recognition · Computer Science 2026-02-16 Lihe Liu , Xiaoxi Pan , Yinyin Yuan , Lulu Shang

Spatial transcriptomics (ST) is a promising technique that characterizes the spatial gene profiling patterns within the tissue context. Comprehensive ST analysis depends on consecutive slices for 3D spatial insights, whereas the missing…

Image and Video Processing · Electrical Eng. & Systems 2025-05-19 NingFeng Que , Xiaofei Wang , Jingjing Chen , Yixuan Jiang , Chao Li

Spatial transcriptomics (ST) provides high-resolution pathological images and whole-transcriptomic expression profiles at individual spots across whole-slide scales. This setting makes it an ideal data source to develop multimodal…

Computer Vision and Pattern Recognition · Computer Science 2024-11-27 Yuxiang Lin , Ling Luo , Ying Chen , Xushi Zhang , Zihui Wang , Wenxian Yang , Mengsha Tong , Rongshan Yu

Spatial Transcriptomics (ST) profiles thousands of gene expression values at discrete spots with precise coordinates on tissue sections, preserving spatial context essential for clinical and pathological studies. With rising sequencing…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Yishun Zhu , Jiaxin Qi , Jian Wang , Yuhua Zheng , Jianqiang Huang

Human and animal tissues consist of heterogeneous cell types that organize and interact in highly structured manners. Bulk and single-cell sequencing technologies remove cells from their original microenvironments, resulting in a loss of…

Quantitative Methods · Quantitative Biology 2022-02-08 Boxiang Liu , Yanjun Li , Liang Zhang

Spatial transcriptomics (ST) is a novel technique that simultaneously captures pathological images and gene expression profiling with spatial coordinates. Since ST is closely related to pathological features such as disease subtypes, it may…

Computer Vision and Pattern Recognition · Computer Science 2025-03-11 Kazuya Nishimura , Ryoma Bise , Yasuhiro Kojima

Advances in spatially-resolved transcriptomics (SRT) technologies have propelled the development of new computational analysis methods to unlock biological insights. As the cost of generating these data decreases, these technologies provide…

Genomics · Quantitative Biology 2024-08-02 Boyi Guo , Wodan Ling , Sang Ho Kwon , Pratibha Panwar , Shila Ghazanfar , Keri Martinowich , Stephanie C. Hicks

The integration of spatial multi-omics data from single tissues is crucial for advancing biological research. However, a significant data imbalance impedes progress: while spatial transcriptomics data is relatively abundant, spatial…

Artificial Intelligence · Computer Science 2026-02-06 Zhaorui Jiang , Yingfang Yuan , Lei Hu , Wei Pang

Spatial transcriptomics (ST) technologies enable gene expression profiling with spatial resolution, offering unprecedented insights into tissue organization and disease heterogeneity. However, current analysis methods often struggle with…

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