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Biomolecular condensates are small droplets forming spontaneously in biological cells via phase separation. They play a role in many cellular processes, but it is unclear how cells control them. Cellular regulation often relies on…

Soft Condensed Matter · Physics 2024-03-15 Jan Kirschbaum , David Zwicker

Biomolecular condensates play a central role in the spatial organization of living matter. Their formation is now well understood as a form of liquid-liquid phase separation that occurs very far from equilibrium. For instance, they can be…

Soft Condensed Matter · Physics 2024-10-14 Roxanne Berthin , Jacques Fries , Marie Jardat , Vincent Dahirel , Pierre Illien

Biomolecular condensates are essential for cellular organization and result from phase separation in systems far from thermodynamic equilibrium. Among various models, chemically active droplets play a significant role, consisting of…

Soft Condensed Matter · Physics 2025-05-19 Jacques Fries , Roxanne Berthin , Chengjie Luo , Marie Jardat , David Zwicker , Vincent Dahirel , Pierre Illien

The formation of condensates is now considered as a major organization principle of eukaryotic cells. Several studies have recently shown that the properties of these condensates are affected by enzymatic reactions. We propose here a simple…

Soft Condensed Matter · Physics 2024-11-19 Jacques Fries , Javier Diaz , Marie Jardat , Ignacio Pagonabarraga , Pierre Illien , Vincent Dahirel

Phase-separated liquid condensates can spatially organize and thereby regulate chemical processes. However, the physicochemical mechanisms underlying such regulation remain elusive as the intramolecular interactions responsible for phase…

Soft Condensed Matter · Physics 2024-03-11 Sudarshana Laha , Jonathan Bauermann , Frank Jülicher , Thomas C. T. Michaels , Christoph A. Weber

Biomolecular condensates provide distinct chemical environments, which control various cellular processes. The diffusive dynamics and chemical kinetics inside phase-separated condensates can be studied experimentally by fluorescently…

Subcellular Processes · Quantitative Biology 2025-03-26 Stefano Bo , Lars Hubatsch , Frank Jülicher

Living cells exhibit a complex organization comprising numerous compartments, among which are RNA- and protein-rich membraneless, liquid-like organelles known as biomolecular condensates. Energy-consuming processes regulate their formation…

Biomolecular condensates form by phase separation of biological polymers and have important functions in the cell $-$ functions that are inherently connected to their physical properties. A remarkable aspect of such condensates is that…

In living cells, proteins involved in specialized biochemical functions are often spatially organized within biomolecular condensates. Increasing evidence suggests that some of these condensates, including DNA repair condensates, emerge…

Soft Condensed Matter · Physics 2026-05-15 Léa Beaulès , Judith Miné-Hattab , Pierre Illien , Vincent Dahirel

Biomolecular condensates help organize the cell cytoplasm and nucleoplasm into spatial compartments with different chemical compositions. A key feature of such compositional patterning is the local enrichment of enzymatically active…

Biological Physics · Physics 2024-08-06 Andriy Goychuk , Leonardo Demarchi , Ivan Maryshev , Erwin Frey

Biomolecular condensates are formed via liquid-liquid phase separation of proteins, often together with nucleic acids, typically driven by interactions between low-affinity binding sites. The computational study of such condensates that…

Soft Condensed Matter · Physics 2025-01-16 Alena Taskina , Devika Magan , Simon Dannenberg , Stefan Klumpp

One of the hypothesized functions of biomolecular condensates is to act as chemical reactors, where chemical reactions can be modulated, i.e. accelerated or slowed down, while substrate molecules enter and products exit from the condensate.…

Biological Physics · Physics 2024-07-02 Alexandra Shelest , Hugo Le Roy , Daniel M. Busiello , Paolo De Los Rios

Phase-separated biomolecular condensates exhibit a wide range of dynamical properties, which depend on the sequences of the constituent proteins and RNAs. However, it is unclear to what extent condensate dynamics can be tuned without also…

Biomolecules · Quantitative Biology 2024-07-31 Yaxin An , Michael A. Webb , William M. Jacobs

Chemical activity is known to affect phase coexistence and coarsening in liquid mixtures, most commonly through reaction-induced changes of intermolecular interactions. Here, we analyze a scenario in which chemical reactions regulate…

Soft Condensed Matter · Physics 2026-03-19 Florian Raßhofer , Erwin Frey

Biomolecular condensates are complex droplets comprising diverse molecules that interact using various mechanisms. Condensation is often driven by short-ranged attraction, but net charges can also mediate long-ranged repulsion. Using…

Soft Condensed Matter · Physics 2025-01-23 Chengjie Luo , Nathaniel Hess , Dilimulati Aierken , Yicheng Qiang , Jerelle A. Joseph , David Zwicker

Active transport of biomolecular condensates and cell migration in collectives are fundamental to development, homeostasis, and processes such as cancer progression, wound healing, and infection response. Yet how these assemblies are…

Soft Condensed Matter · Physics 2025-10-24 Hossein Vahid , Jens-Uwe Sommer , Abhinav Sharma

Driven chemical reactions can control the macroscopic properties of droplets, like their size. Such active droplets are critical in structuring the interior of biological cells. Cells also need to control where and when droplets appear, so…

Soft Condensed Matter · Physics 2024-03-15 Noah Ziethen , Jan Kirschbaum , David Zwicker

Biomolecular condensates constitute a newly recognized form of spatial organization in living cells. Although many condensates are believed to form as a result of phase separation, the physicochemical properties that determine the phase…

Biological Physics · Physics 2023-07-11 William M. Jacobs

The interaction of phase-separating systems with chemical reactions is of great interest in various contexts, from biology to material science. In biology, phase separation is thought to be the driving force behind the formation of…

Soft Condensed Matter · Physics 2026-01-08 Franco Blanchini , Elisa Franco , Giulia Giordano , Dino Osmanovic

Cells regulate gene expression in part by forming DNA-protein condensates in the nucleus. While existing theories describe the equilibrium size and stability of such condensates, their dynamics remain less understood. Here, we use…

Soft Condensed Matter · Physics 2025-12-22 Adam R. Lamson , Mohammadhossein Firouznia , Michael J. Shelley
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