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Protein molecules in cells are synthesized by macromolecular machines called ribosomes. According to recent experimental data, we reduce the complexity of the ribosome and propose a model to express its activity in six main states. Using…

Subcellular Processes · Quantitative Biology 2016-07-26 Bahareh Shakiba , Maryam Dayeri , Farshid Mohammad-Rafiee

Cellular differentiation is governed by gene regulatory networks, the high-dimensional stochastic biochemical systems that determine the transcriptional landscape and mediate cellular responses to signals and perturbations. Although…

Molecular Networks · Quantitative Biology 2026-04-29 Suryanarayana Maddu , Victor Chardès , Michael J. Shelley

mRNA translation is a crucial process that leads to protein synthesis in living cells. Therefore, it is a process that needs to work optimally for a cell to stay healthy and alive. With advancements in microscopy and novel experimental…

Subcellular Processes · Quantitative Biology 2023-12-20 Binil Shyam T , Rati Sharma

Ribosome is a molecular machine that polymerizes a protein where the sequence of the amino acid residues, the monomers of the protein, is dictated by the sequence of codons (triplets of nucleotides) on a messenger RNA (mRNA) that serves as…

Biological Physics · Physics 2011-01-28 Ajeet K. Sharma , Debashish Chowdhury

In many important cellular processes, including mRNA translation, gene transcription, phosphotransfer, and intracellular transport, biological "particles" move along some kind of "tracks". The motion of these particles can be modeled as a…

Subcellular Processes · Quantitative Biology 2017-11-01 Yoram Zarai , Michael Margaliot , Tamir Tuller

We develop a stochastic model for the programmed frameshift of ribosomes synthesizing a protein while moving along a mRNA template. Normally the reading frame of a ribosome decodes successive triplets of nucleotides on the mRNA in a…

Biological Physics · Physics 2017-06-01 Bhavya Mishra , Gunter M. Schütz , Debashish Chowdhury

The molecular network in an organism consists of transcription/translation regulation, protein-protein interactions/modifications and a metabolic network, together forming a system that allows the cell to respond sensibly to the multiple…

Molecular Networks · Quantitative Biology 2007-05-23 Sandeep Krishna , Anna M. C. Andersson , Szabolcs Semsey , Kim Sneppen

We explore and quantify the physical and biochemical mechanisms that may be relevant in the regulation of translation. After elongation and detachment from the 3' termination site of mRNA, parts of the ribosome machinery can diffuse back to…

Soft Condensed Matter · Physics 2009-11-10 Tom Chou

The ribosome is one of the largest and most complex macromolecular machines in living cells. It polymerizes a protein in a step-by-step manner as directed by the corresponding nucleotide sequence on the template messenger RNA (mRNA) and…

Statistical Mechanics · Physics 2020-03-11 Annwesha Dutta , Gunter M Schütz , Debashish Chowdhury

Synthesis of protein molecules in a cell are carried out by ribosomes. A ribosome can be regarded as a molecular motor which utilizes the input chemical energy to move on a messenger RNA (mRNA) track that also serves as a template for the…

Biological Physics · Physics 2009-07-12 Ashok Garai , Debanjan Chowdhury , Debashish Chowdhury , T. V. Ramakrishnan

Many {\it ribosomes} simultaneously move on the same messenger RNA (mRNA), each synthesizing separately a copy of the same protein. In contrast to the earlier models, here {\it we develop a ``unified'' theoretical model} that not only…

Biological Physics · Physics 2007-05-23 Aakash Basu , Debashish Chowdhury

The rate of mRNA translation depends on the initiation, elongation, and termination rates of ribosomes along the mRNA. These rates depend on many "local" factors like the abundance of free ribosomes and tRNA molecules in the vicinity of the…

Subcellular Processes · Quantitative Biology 2021-02-25 Michael Margaliot , Wasim Huleihel , Tamir Tuller

The process of polymerizing a protein by a ribosome, using a messenger RNA (mRNA) as the corresponding template, is called {\it translation}. Ribosome may be regarded as a molecular motor for which the mRNA template serves also as the…

Biological Physics · Physics 2015-03-19 Ajeet K. Sharma , Debashish Chowdhury

Translation of mRNA into protein is a fundamental yet complex biological process with multiple factors that can potentially affect its efficiency. Here, we study a stochastic model describing the traffic flow of ribosomes along the mRNA…

Mathematical Physics · Physics 2020-01-23 Dan D. Erdmann-Pham , Khanh Dao Duc , Yun S. Song

Organisms are equipped with regulatory systems that display a variety of dynamical behaviours ranging from simple stable steady states, to switching and multistability, to oscillations. Earlier work has shown that oscillations in protein…

Molecular Networks · Quantitative Biology 2009-11-13 Simone Pigolotti , Sandeep Krishna , Mogens H. Jensen

The growing significance of RNA engineering in diverse biological applications has spurred interest in developing AI methods for structure-based RNA design. While diffusion models have excelled in protein design, adapting them for RNA…

Biomolecules · Quantitative Biology 2024-06-11 Divya Nori , Wengong Jin

Proteins are polymerized by cyclic machines called ribosome which use their messenger RNA (mRNA) track also as the corresponding template and the process is called translation. We explore, in depth and detail, the stochastic nature of the…

Biological Physics · Physics 2009-11-13 Ashok Garai , Debashish Chowdhury , T. V. Ramakrishnan

The translation of messenger RNA transcripts to proteins is commonly modeled as a one-dimensional totally asymmetric exclusion process with extended particles. Here we focus on the effects of premature termination of translation through the…

Subcellular Processes · Quantitative Biology 2023-08-31 Johannes Keisers , Joachim Krug

Individual mRNAs are translated by multiple ribosomes that initiate translation with a few seconds interval. The ribosome speed is codon dependant, and ribosome queuing has been suggested to explain specific data for translation of some…

Subcellular Processes · Quantitative Biology 2008-09-26 Namiko Mitarai , Kim Sneppen , Steen Pedersen

Recent advances in DNA sequencing and fluorescence imaging have made it possible to monitor the dynamics of ribosomes actively engaged in messenger RNA (mRNA) translation. Here, we model these experiments within the inhomogeneous totally…

Biological Physics · Physics 2020-07-01 Juraj Szavits-Nossan , Martin R. Evans