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Super-resolution imaging techniques have largely improved our capabilities to visualize nanometric structures in biological systems. Their application further enables one to potentially quantitate relevant parameters to determine the…

Biological Physics · Physics 2019-12-18 Tina Kosǔta , Marta Cullell-Dalmau , Francesca Cella Zanacchi , Carlo Manzo

Unprecedented atomic-scale measurement resolution has recently been demonstrated in single-shot optical localization metrology based on deep-learning analyses of diffraction patterns of topologically structured light scattered from objects.…

High-resolution imaging of ultracold atoms typically requires custom high numerical aperture (NA) optics, as is the case for quantum gas microscopy. These high NA objectives involve many optical elements each of which contributes to loss…

Quantum Gases · Physics 2021-11-01 Emine Altuntas , Ian B. Spielman

Atomic resolution imaging in transmission electron microscopy (TEM) and scanning TEM (STEM) of light elements in electron-transparent materials has long been a challenge. Biomolecular materials, for example, are rapidly altered when…

Instrumentation and Detectors · Physics 2018-12-05 Fehmi S. Yasin , Tyler R. Harvey , Jordan J. Chess , Jordan S. Pierce , Colin Ophus , Peter Ercius , Benjamin J. McMorran

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

The localisation of fluorophores is an important aspect of the determination of the biological function of cellular systems. Quantum correlation microscopy (QCM) is a promising technique for providing diffraction unlimited emitter…

Quantum Physics · Physics 2023-10-05 Shuo Li , Wenchao Li , Qiang Sun , Bill Moran , Timothy C. Brown , Brant C. Gibson , Andrew D. Greentree

To overcome the limit of diffraction while achieving the superresolution technique, solid immersion lenses are the key optical elements for data storage and nanophotonics applications. Recent demonstrations have shown how different…

Optics · Physics 2026-02-24 Subhankar Roy , Jianping Hu , M Ummal Momeen

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and…

Biomolecules · Quantitative Biology 2015-06-24 Zhaokun Zhou , Helen Miller , Adam J. M. Wollman , Mark C. Leake

Automated tumor detection in Digital Breast Tomosynthesis (DBT) is a difficult task due to natural tumor rarity, breast tissue variability, and high resolution. Given the scarcity of abnormal images and the abundance of normal images for…

Image and Video Processing · Electrical Eng. & Systems 2024-07-24 Nicholas Konz , Haoyu Dong , Maciej A. Mazurowski

Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…

Optics · Physics 2012-03-21 Meng Cui , Ke Si , Reto Fiolka

In Ultrasound Localization Microscopy (ULM), achieving high-resolution images relies on the precise localization of contrast agent particles across a series of beamformed frames. However, our study uncovers an enormous potential: The…

Computational Geometry · Computer Science 2024-04-09 Christopher Hahne , Georges Chabouh , Arthur Chavignon , Olivier Couture , Raphael Sznitman

The Abbe's diffraction limit restricts the resolution of an optical imaging and lithography system. Coherent Rabi oscillation is shown to be able to overcome the diffraction limit in both optical and atom lithography. In previous studies,…

Quantum Physics · Physics 2018-04-04 Anqi Zhang , Zeyang Liao , Rongxin Chen , Da-Wei Wang

We overcome the diffraction limit in fluorescence imaging of neutral atoms in a sparsely filled one-dimensional optical lattice. At a periodicity of 433 nm, we reliably infer the separation of two atoms down to nearest neighbors. We observe…

Quantum Physics · Physics 2009-02-06 M. Karski , L. Förster , J. M. Choi , W. Alt , A. Widera , D. Meschede

We consider a method of high-fidelity, spatially resolved position measurement of ultracold atoms in an optical lattice. We show that the atom-number distribution can be nondestructively determined at a spatial resolution beyond the…

Quantum Gases · Physics 2015-08-28 Yuto Ashida , Masahito Ueda

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

The diffraction limited resolution of light focused by a lens was derived in 1873 by Ernst Abbe. Later in 1952, a method to reach sub-diffraction light spots was proposed by modulating the wavefront of the focused beam. In a related…

Optics · Physics 2018-03-20 Brijesh Kumar Singh , Harel Nagar , Yael Roichman , Ady Arie

Quantum metrology exploits quantum resources to enhance measurement precision beyond the classical limit. Conventional protocols normally rely on the preparation of delicate quantum states to acquire these resources, posing a major…

Quantum Physics · Physics 2026-03-31 Yuan Liu , Ke-Mi Xu , Hong-Bo Sun , Linhan Lin

Optical superresolution microscopy is an important field, where nonlinear optical processes or prior information is used to defeat the classical diffraction limit of light. Quantum correlation microscopy uses photon arrival statistics from…

Optics · Physics 2023-10-13 Jaret J. Vasquez-Lozano , Shuo Li , Andrew D. Greentree

Quantum-gas microscopes are used to study ultracold atoms in optical lattices at the single particle level. In these system atoms are localised on lattice sites with separations close to or below the diffraction limit. To determine the…

Quantum Gases · Physics 2023-11-09 A. La Rooij , C. Ulm , E. Haller , S. Kuhr