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There is only a handful of scanning techniques that can provide surface topography at nanometre resolution. At the same time, there are no methods that are capable of non-invasive imaging of the three-dimensional surface topography of a…

Instrumentation and Detectors · Physics 2017-03-07 Tatiana Latychevskaia , Wei-Hao Hsu , Wei-Tse Chang , Chun-Yueh Lin , Ing-Shouh Hwang

This preliminary study presents a simple modulation scheme to dynamically create time-averaged optical potentials to trap colloidal particles using acousto-optical modulation. The method provides access to control experimentally relevant…

Soft Condensed Matter · Physics 2022-04-21 Thalyta Tavares Martins , Sérgio Ricardo Muniz

Protein rotational kinetics are essential for understanding macromolecular behavior in crowded environments, yet measuring these dynamics at solid-liquid interfaces remains a significant challenge due to low signal strengths. Here, we…

Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Daniel Freedman , Racheli Gordon , Boris Ferdman , Lucien E. Weiss , Onit Alalouf , Reut Orange , Tomer Michaeli , Yoav Shechtman

Focused electron beam induced deposition (FEBID) is a powerful technique for 3D-printing of complex nanodevices. However, for resolutions below 10 nm, it struggles to control size, morphology and composition of the structures, due to a lack…

Reconstruction based on the stereo camera has received considerable attention recently, but two particular challenges still remain. The first concerns the need to aggregate similar pixels in an effective approach, and the second is to…

Computer Vision and Pattern Recognition · Computer Science 2017-03-31 Lei Fan , Ziyu Pan , Long Chen , Kai Huang

Single particle reconstruction has recently emerged in 3D fluorescence microscopy as a powerful technique to improve the axial resolution and the degree of fluorescent labeling. It is based on the reconstruction of an average volume of a…

Image and Video Processing · Electrical Eng. & Systems 2023-01-24 Thibaut Eloy , Etienne Baudrier , Marine Laporte , Virginie Hamel , Paul Guichard , Denis Fortun

We extend our previously proposed image reconstruction method, which allows confocal microscopes to capture periodically moving objects at frequencies beyond their frame rates, to three-dimensional and two-dimensional wide-field imaging.…

Optics · Physics 2026-03-03 Kanta Adachi , Soyoka Hemmi , Nobutomo Nakamura

The dynamics of biomolecules are crucial for our understanding of their functioning in living systems. However, current 3D imaging techniques, such as cryogenic electron microscopy (cryo-EM), require freezing the sample, which limits the…

Quantitative Methods · Quantitative Biology 2023-08-24 Enze Ye , Yuhang Wang , Hong Zhang , Yiqin Gao , Huan Wang , He Sun

The manipulation of nanoscale matter has the potential to revolutionize a variety of fields across nanoscience and technology. Here, we demonstrate experimentally and characterize numerically a device that combines the benefits of…

Mesoscale and Nanoscale Physics · Physics 2024-08-13 Marco Riccardi , Olivier J. F. Martin

Real time visualization and tracking of colloidal particles with 3D resolution is essential for probing the local structure and dynamics in complex fluids. Although tracking translational motion of spherical colloids is well-known,…

Applied Physics · Physics 2020-05-19 Beybin Ilhan , Jelle J. Schoppink , Frieder Mugele , Michael H. G. Duits

Proteins are the active working horses in our body. These biomolecules perform all vital cellular functions from DNA replication and general biosynthesis to metabolic signaling and environmental sensing. While static 3D structures are now…

Quantitative Methods · Quantitative Biology 2020-10-16 Sonja Schmid , Cees Dekker

In situ electron microscopy is a key tool for understanding the mechanisms driving novel phenomena in 2D structures. Unfortunately, due to various practical challenges, technologically relevant 2D heterostructures prove challenging to…

Three-dimensional electron diffraction (3D ED) has emerged as a powerful method for solving the structures of sub-micron-sized particles down to nanoparticles. However, it faces technical challenges when applied to beam-sensitive samples or…

Capture, selective collection and flight manipulation of airborne particulate are three important functional requirements in various actively growing aerosol technology applications. Aerodynamic drag, particle inertia and dielectrophoretic…

Soft Condensed Matter · Physics 2022-03-16 Pramodt Srinivasula , Rochish M Thaokar

We present a general paradigm for dynamic 3D reconstruction from multiple independent and uncontrolled image sources having arbitrary temporal sampling density and distribution. Our graph-theoretic formulation models the Spatio-temporal…

Computer Vision and Pattern Recognition · Computer Science 2019-08-30 Xiangyu Xu , Enrique Dunn

Single-particle electron cryomicroscopy is an essential tool for high-resolution 3D reconstruction of proteins and other biological macromolecules. An important challenge in cryo-EM is the reconstruction of non-rigid molecules with parts…

Image and Video Processing · Electrical Eng. & Systems 2021-05-31 Amit Moscovich , Amit Halevi , Joakim Andén , Amit Singer

Colloidal assembly represents a powerful method for the fabrication of functional materials. In this article, we describe how acoustic radiation forces can guide the assembly of colloidal particles into structures that serve as microscopic…

Soft Condensed Matter · Physics 2016-03-17 Mihai Caleap , Bruce Drinkwater

Silicon-based photonic biosensors, such as microring resonators and Mach-Zehnder interferometers, offer significant potential for the detection of analytes at low concentrations. To enhance response time and improve the limit of detection…

Optics · Physics 2024-07-01 Anders Henriksson , Peter Neubauer , Mario Birkholz

Colloidal lithography [1] is how patterns are reproduced in a variety of natural systems and is used more and more as an efficient fabrication tool in bio-, opto-, and nano-technology. Nanoparticles in the colloid are made to form a mask on…

Mesoscale and Nanoscale Physics · Physics 2013-11-27 A. Iovan , M. Fischer , R. Lo Conte , V. Korenivski