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In fluorescence microscopy, spectral unmixing aims to recover individual fluorophore concentrations from spectral images that capture mixed fluorophore emissions. Since classical methods operate pixel-wise and rely on least-squares fitting,…

Computer Vision and Pattern Recognition · Computer Science 2026-03-26 Federico Carrara , Talley Lambert , Mehdi Seifi , Florian Jug

In this work, we present denoiSplit, a method to tackle a new analysis task, i.e. the challenge of joint semantic image splitting and unsupervised denoising. This dual approach has important applications in fluorescence microscopy, where…

Image and Video Processing · Electrical Eng. & Systems 2024-08-13 Ashesh Ashesh , Florian Jug

We present {\mu}Split, a dedicated approach for trained image decomposition in the context of fluorescence microscopy images. We find that best results using regular deep architectures are achieved when large image patches are used during…

Computer Vision and Pattern Recognition · Computer Science 2023-08-21 Ashesh , Alexander Krull , Moises Di Sante , Francesco Silvio Pasqualini , Florian Jug

Single-pixel imaging has emerged as a key technique in fluorescence microscopy, where fast acquisition and reconstruction are crucial. In this context, images are reconstructed from linearly compressed measurements. In practice, total…

Image and Video Processing · Electrical Eng. & Systems 2025-07-28 Serban C. Tudosie , Valerio Gandolfi , Shivaprasad Varakkoth , Andrea Farina , Cosimo D'Andrea , Simon Arridge

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

The learned image compression (LIC) methods have already surpassed traditional techniques in compressing natural scene (NS) images. However, directly applying these methods to screen content (SC) images, which possess distinct…

Image and Video Processing · Electrical Eng. & Systems 2025-02-24 Shiqi Jiang , Hui Yuan , Shuai Li , Huanqiang Zeng , Sam Kwong

Blind image decomposition aims to decompose all components present in an image, typically used to restore a multi-degraded input image. While fully recovering the clean image is appealing, in some scenarios, users might want to retain…

Computer Vision and Pattern Recognition · Computer Science 2024-03-18 Zeyu Zhang , Junlin Han , Chenhui Gou , Hongdong Li , Liang Zheng

The trade-off between throughput and image quality is an inherent challenge in microscopy. To improve throughput, compressive imaging under-samples image signals; the images are then computationally reconstructed by solving a regularized…

Image and Video Processing · Electrical Eng. & Systems 2023-03-07 Udith Haputhanthri , Andrew Seeber , Dushan Wadduwage

Fluorescence microscopy allows for a detailed inspection of cells, cellular networks, and anatomical landmarks by staining with a variety of carefully-selected markers visualized as color channels. Quantitative characterization of…

Computer Vision and Pattern Recognition · Computer Science 2021-08-26 Alvaro Gomariz , Tiziano Portenier , Patrick M. Helbling , Stephan Isringhausen , Ute Suessbier , César Nombela-Arrieta , Orcun Goksel

Current self-supervised denoising methods for paired noisy images typically involve mapping one noisy image through the network to the other noisy image. However, after measuring the spectral bias of such methods using our proposed Image…

Computer Vision and Pattern Recognition · Computer Science 2025-10-02 Wang Zhang , Huaqiu Li , Xiaowan Hu , Tao Jiang , Zikang Chen , Haoqian Wang

Image decomposition is a crucial subject in the field of image processing. It can extract salient features from the source image. We propose a new image decomposition method based on convolutional neural network. This method can be applied…

Computer Vision and Pattern Recognition · Computer Science 2022-08-04 Yu Fu , Xiao-Jun Wu , Josef Kittler

Few-shot Semantic Segmentation (FSS) is a challenging task that utilizes limited support images to segment associated unseen objects in query images. However, recent FSS methods are observed to perform worse, when enlarging the number of…

Computer Vision and Pattern Recognition · Computer Science 2025-01-24 Wailing Tang , Biqi Yang , Pheng-Ann Heng , Yun-Hui Liu , Chi-Wing Fu

Although image restoration has advanced significantly, most existing methods target only a single type of degradation. In real-world scenarios, images often contain multiple degradations simultaneously, such as rain, noise, and haze,…

Computer Vision and Pattern Recognition · Computer Science 2025-11-14 Hu Gao , Xiaoning Lei , Xichen Xu , Depeng Dang , Lizhuang Ma

One common task in image forensics is to detect spliced images, where multiple source images are composed to one output image. Most of the currently best performing splicing detectors leverage high-frequency artifacts. However, after an…

Computer Vision and Pattern Recognition · Computer Science 2022-06-23 Benjamin Hadwiger , Christian Riess

In this paper, we propose an image compression algorithm called Microshift. We employ an algorithm hardware co-design methodology, yielding a hardware-friendly compression approach with low power consumption. In our method, the image is…

Image and Video Processing · Electrical Eng. & Systems 2021-04-21 Bo Zhang , Pedro V. Sander , Chi-Ying Tsui , Amine Bermak

Fluorescence lifetime imaging microscopy (FLIM) is a powerful quantitative technique that provides metabolic and molecular contrast, offering strong translational potential for label-free, real-time diagnostics. However, its clinical…

Computer Vision and Pattern Recognition · Computer Science 2025-12-19 Paloma Casteleiro Costa , Parnian Ghapandar Kashani , Xuhui Liu , Alexander Chen , Ary Portes , Julien Bec , Laura Marcu , Aydogan Ozcan

Deep learning-based image fusion approaches have obtained wide attention in recent years, achieving promising performance in terms of visual perception. However, the fusion module in the current deep learning-based methods suffers from two…

Computer Vision and Pattern Recognition · Computer Science 2022-02-01 Dongyu Rao , Xiao-Jun Wu , Tianyang Xu , Guoyang Chen

In the practical application of restoring low-resolution gray-scale images, we generally need to run three separate processes of image colorization, super-resolution, and dows-sampling operation for the target device. However, this pipeline…

Computer Vision and Pattern Recognition · Computer Science 2022-01-13 Jiangning Zhang , Chao Xu , Jian Li , Yue Han , Yabiao Wang , Ying Tai , Yong Liu

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

Visualizing the details of different cellular structures is of great importance to elucidate cellular functions. However, it is challenging to obtain high quality images of different structures directly due to complex cellular environments.…

Image and Video Processing · Electrical Eng. & Systems 2019-10-01 Yi Liu , Hao Yuan , Zhengyang Wang , Shuiwang Ji
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