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We propose a new learning-based approach for 3D particle field imaging using holography. Our approach uses a U-net architecture incorporating residual connections, Swish activation, hologram preprocessing, and transfer learning to cope with…

Image and Video Processing · Electrical Eng. & Systems 2020-02-19 Siyao Shao , Kevin Mallery , Santosh Kumar , Jiarong Hong

We propose a speed-up method for the in-focus plane detection in digital holographic microscopy that can be applied to a broad class of autofocusing algorithms that involve repetitive propagation of an object wave to various axial locations…

Quantitative Methods · Quantitative Biology 2023-05-19 Julianna Winnik , Damian Suski , Piotr Zdańkowski , Luiza Stanaszek , Vicente Micó , Maciej Trusiak

Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…

Optics · Physics 2024-11-26 Shyam Kumar , Jiarong Hong

Confocal microscopy of colloids combined with digital image processing has become a powerful tool in soft matter physics and materials science. Together, these techniques enable locating and tracking of more than half a million individual…

Soft Condensed Matter · Physics 2016-05-10 Katharine E. Jensen , Nobutomo Nakamura

We introduce a novel framework that incorporates multiple scattering for large-scale 3D particle-localization using single-shot in-line holography. Traditional holographic techniques rely on single-scattering models which become inaccurate…

Signal Processing · Electrical Eng. & Systems 2019-06-19 Waleed Tahir , Ulugbek S. Kamilov , Lei Tian

We propose a three-dimensional nonlinear diffusion method to implement the similar autofocusing function of multiple micro-objects and simultaneously remove the defocused images, which can distinguish the locations of certain sized…

Computer Vision and Pattern Recognition · Computer Science 2019-08-15 Wei-Na Li , Zhengyun Zhang , Jianshe Ma , Xiaohao Wang , Ping Su

In complex plasmas, the behavior of freely floating micrometer sized particles is studied. The particles can be directly visualized and recorded by digital video cameras. To analyze the dynamics of single particles, reliable algorithms are…

Plasma Physics · Physics 2018-02-14 Daniel P. Mohr , Christina A. Knapek , Peter Huber , Erich Zaehringer

Confocal microscopy in combination with real-space particle tracking has proven to be a powerful tool in scientific fields such as soft matter physics, materials science and cell biology. However, 3D tracking of anisotropic particles in…

Soft Condensed Matter · Physics 2015-05-20 T. H. Besseling , M. Hermes , A. Kuijk , B. de Nijs , T. -S. Deng , M. Dijkstra , A. Imhof , A. van Blaaderen

We develop a novel algorithm for large-scale holographic reconstruction of 3D particle fields. Our method is based on a multiple-scattering beam propagation method (BPM) combined with sparse regularization that enables recovering dense 3D…

Optics · Physics 2021-05-24 Hao Wang , Waleed Tahir , Jiabei Zhu , Lei Tian

Confocal microscopy of fluorescent labeled particles has been used to study the dynamical and structural properties of colloidal and granular matter in real space. Localization algorithms allow for a fully automatized determination of the…

Soft Condensed Matter · Physics 2011-12-08 Marcel Roth , Michael Franzmann , Maria D'Acunzi , Max Kreiter , Günter K. Auernhammer

Digital holographic microscopy is a fast three-dimensional (3D) imaging tool with many applications in soft matter physics. Recent studies have shown that electromagnetic scattering solutions can be fit to digital holograms to obtain the 3D…

Accurately tracking particles and determining their coordinate along the optical axis is a major challenge in optical microscopy, especially when extremely high precision is needed. In this study, we introduce a deep learning approach using…

Image and Video Processing · Electrical Eng. & Systems 2025-08-05 Andrey Alexandrov , Giovanni Acampora , Giovanni De Lellis , Antonia Di Crescenzo , Chiara Errico , Daria Morozova , Valeri Tioukov , Autilia Vittiello

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

Instrumentation and Detectors · Physics 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

A method for particle orientation tracking is developed and demonstrated specifically for anisotropic particles. Using (high-speed) multi-camera recordings of anisotropic particles from different viewpoints, we reconstruct the 3D location…

Computer Vision and Pattern Recognition · Computer Science 2025-05-13 Mees M. Flapper , Elian Bernard , Sander G. Huisman

This paper proposes a particle volume reconstruction directly from an in-line hologram using a deep neural network. Digital holographic volume reconstruction conventionally uses multiple diffraction calculations to obtain sectional…

Image and Video Processing · Electrical Eng. & Systems 2019-03-27 Tomoyoshi Shimobaba , Takayuki Takahashi , Yota Yamamoto , Yutaka Endo , Atsushi Shiraki , Takashi Nishitsuji , Naoto Hoshikawa , Takashi Kakue , Tomoyosh Ito

Among the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually,the…

Optics · Physics 2024-07-11 Hanzhe Wu , Danni Chen , YiHong Jiand Gan Xiang , Heng Li , Bin Yu , JunLe Qu

Advanced three-dimensional (3D) tracking methods are essential for studying particle dynamics across a wide range of complex systems, including multiphase flows, environmental and atmospheric sciences, colloidal science, biological and…

Fluid Dynamics · Physics 2024-12-25 Shyam Kumar M , Jiarong Hong

The development of robust, real-time optical methods for the detection and tracking of particles in complex multiple scattering media is a problem of practical importance in a number of fields, including environmental monitoring, air…

Optics · Physics 2025-02-04 Mohammadrasoul Taghavi , Edwin A. Marengo

Reconstructing the 3D location and size of microparticles from diffraction images - holograms - is a computationally expensive inverse problem that has traditionally been solved using physics-based reconstruction methods. More recently,…

Computer Vision and Pattern Recognition · Computer Science 2025-03-17 Ayush Paliwal , Oliver Schlenczek , Birte Thiede , Manuel Santos Pereira , Katja Stieger , Eberhard Bodenschatz , Gholamhossein Bagheri , Alexander Ecker

Three-dimensional particle tracking is an essential tool in studying dynamics under the microscope, namely, fluid dynamics in microfluidic devices, bacteria taxis, cellular trafficking. The 3d position can be determined using 2d imaging…

Computer Vision and Pattern Recognition · Computer Science 2015-09-08 Eldad Afik
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