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The intricate interplay between host organisms and their gut microbiota has catalyzed research into the microbiome's role in disease, shedding light on novel aspects of disease pathogenesis. However, the mechanisms through which the…

Methodology · Statistics 2024-02-20 Lei Fang , Yue Wang , Chenglong Ye

Background. Emerging technologies now allow for mass spectrometry based profiling of up to thousands of small molecule metabolites (metabolomics) in an increasing number of biosamples. While offering great promise for revealing insight into…

Metabolic heterogeneity is widely recognised as the next challenge in our understanding of non-genetic variation. A growing body of evidence suggests that metabolic heterogeneity may result from the inherent stochasticity of intracellular…

Molecular Networks · Quantitative Biology 2020-10-08 Mona K Tonn , Philipp Thomas , Mauricio Barahona , Diego A Oyarzún

We investigate the effect of cadmium (a toxic environmental pollutant) on the correlation structure of a number of urinary metabolites using Gaussian graphical models (GGMs). The inferred metabolic associations can provide important…

Methodology · Statistics 2019-04-23 Linda S. L. Tan , Ajay Jasra , Maria De Iorio , Timothy M. D. Ebbels

Mediation analysis is a statistical approach that can provide insights regarding the intermediary processes by which an intervention or exposure affects a given outcome. Mediation analyses rose to prominence, particularly in social science…

Mediation analysis is a powerful tool for studying causal pathways between exposure, mediator, and outcome variables of interest. While classical mediation analysis using observational data often requires strong and sometimes unrealistic…

Methodology · Statistics 2024-05-20 Rita Qiuran Lyu , Chong Wu , Xinwei Ma , Jingshen Wang

The prevalence of chronic non-communicable diseases such as obesity has noticeably increased in the last decade. The study of these diseases in early life is of paramount importance in determining their course in adult life and in…

Mediation analysis aims to decipher the underlying causal mechanisms between an exposure, an outcome, and intermediate variables called mediators. Initially developed for fixed-time mediator and outcome, it has been extended to the…

Methodology · Statistics 2025-01-15 K. Le Bourdonnec , L. Valeri , C. Proust-Lima

Mediation analysis allows one to use observational data to estimate the importance of each potential mediating pathway involved in the causal effect of an exposure on an outcome. However, current approaches to mediation analysis with…

Mediation analysis has been widely used to investigate how a treatment influences an outcome through intermediate variables, known as mediators. Analyzing a mediation mechanism typically requires assessing multiple model parameters that…

Methodology · Statistics 2025-10-01 Hanying Jiang , Kris Sankaran , Yinqiu He

Causal mediation analysis is a powerful tool in environmental health research, allowing researchers to uncover the pathways through which exposures influence health outcomes. While traditional mediation methods have been widely applied to…

Analyses of causal mediation often involve exposure-induced confounders or, relatedly, multiple mediators. In such applications, researchers aim to estimate a variety of different quantities, including interventional direct and indirect…

Methodology · Statistics 2025-06-18 Jesse Zhou , Geoffrey T. Wodtke

Mediation analysis is concerned with the decomposition of the total effect of an exposure on an outcome into the indirect effect through a given mediator, and the remaining direct effect. This is ideally done using longitudinal measurements…

Statistics Theory · Mathematics 2019-12-04 Murthy N Mittinty , Stijn Vansteelandt

Mediation analysis plays a crucial role in causal inference as it can investigate the pathways through which treatment influences outcome. Most existing mediation analysis assumes that mediation effects are static and homogeneous within…

Methodology · Statistics 2026-03-02 Yijiao Zhang , Yubai Yuan , Yuexia Zhang , Zhongyi Zhu , Annie Qu

Technological advancements in noninvasive imaging facilitate the construction of whole brain interconnected networks, known as brain connectivity. Existing approaches to analyze brain connectivity frequently disaggregate the entire network…

Methodology · Statistics 2023-09-26 Xinyuan Tian , Fan Li , Li Shen , Denise Esserman , Yize Zhao

Mediation analysis breaks down the causal effect of a treatment on an outcome into an indirect effect, acting through a third group of variables called mediators, and a direct effect, operating through other mechanisms. Mediation analysis…

Applications · Statistics 2025-05-13 Judith Abécassis , Houssam Zenati , Sami Boumaïza , Julie Josse , Bertrand Thirion

Mediation analytics help examine if and how an intermediate variable mediates the influence of an exposure variable on an outcome of interest. Quantiles, rather than the mean, of an outcome are scientifically relevant to the comparison…

Methodology · Statistics 2024-12-23 Canyi Chen , Yinqiu He , Huixia J. Wang , Gongjun Xu , Peter X. -K. Song

Causal mediation analysis seeks to investigate how the treatment effect of an exposure on outcomes is mediated through intermediate variables. Although many applications involve longitudinal data, the existing methods are not directly…

Applications · Statistics 2021-02-24 Shuxi Zeng , Stacy Rosenbaum , Elizabeth Archie , Susan Alberts , Fan Li

Recent breakthroughs in cancer research have come via the up-and-coming field of pathway analysis. By applying statistical methods to prior known gene and protein regulatory information, pathway analysis provides a meaningful way to…

Genomics · Quantitative Biology 2017-10-11 Yue Zhao

Introduction: To leverage functionality and clinical relevance into understanding systems biology, one needs to understand the pathway of the genetic effects on risk factors/disease through intermediate molecular levels, such as…

Genomics · Quantitative Biology 2018-09-14 Azam Yazdani , Akram Yazdani , Philip L. Lorenzi , Ahmad Samiei