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Spatial transcriptomics (ST) enables gene expression mapping within anatomical context but remains costly and low-throughput. Hematoxylin and eosin (H\&E) staining offers rich morphology yet lacks molecular resolution. We present…

Spatial variable genes (SVGs) reveal critical information about tissue architecture, cellular interactions, and disease microenvironments. As spatial transcriptomics (ST) technologies proliferate, accurately identifying SVGs across diverse…

Applications · Statistics 2025-10-21 Jiawen Chen , Jinwei Zhang , Dongshen Peng , Yutong Song , Aitong Ruan , Yun Li , Didong Li

The recent advancement of spatial transcriptomics (ST) allows to characterize spatial gene expression within tissue for discovery research. However, current ST platforms suffer from low resolution, hindering in-depth understanding of…

Image and Video Processing · Electrical Eng. & Systems 2025-11-05 Xiaofei Wang , Xingxu Huang , Stephen J. Price , Chao Li

Advances in spatial transcriptomics (ST) technologies enable systematic molecular characterization of tumor microenvironment, tumor gradients and gene regulatory networks. Cancer progression is known to vary along pathological gradients,…

Spatial transcriptomics is a modern sequencing technology that allows the measurement of the activity of thousands of genes in a tissue sample and map where the activity is occurring. This technology has enabled the study of the so-called…

Methodology · Statistics 2022-09-15 Andrea Sottosanti , Davide Risso

Spatial Transcriptomics (ST) provides spatially-resolved gene expression, offering crucial insights into tissue architecture and complex diseases. However, its prohibitive cost limits widespread adoption, leading to significant attention on…

Computer Vision and Pattern Recognition · Computer Science 2026-03-27 Taejin Jeong , Joohyeok Kim , Jinyeong Kim , Chanyoung Kim , Seong Jae Hwang

Spatial Transcriptomics is a novel technology that aligns histology images with spatially resolved gene expression profiles. Although groundbreaking, it struggles with gene capture yielding high corruption in acquired data. Given potential…

Computer Vision and Pattern Recognition · Computer Science 2024-09-30 Gabriel Mejia , Daniela Ruiz , Paula Cárdenas , Leonardo Manrique , Daniela Vega , Pablo Arbeláez

Intelligent medical diagnosis has shown remarkable progress based on the large-scale datasets with precise annotations. However, fewer labeled images are available due to significantly expensive cost for annotating data by experts. To fully…

Image and Video Processing · Electrical Eng. & Systems 2023-03-06 Wentao Lei , Lei Liu , Li Liu

Spatial transcriptomics (ST) is a novel technology that enables the observation of gene expression at the resolution of individual spots within pathological tissues. ST quantifies the expression of tens of thousands of genes in a tissue…

Machine Learning · Computer Science 2025-11-25 Kaito Shiku , Kazuya Nishimura , Shinnosuke Matsuo , Yasuhiro Kojima , Ryoma Bise

Spatial transcriptomics data analysis integrates cellular transcriptional activity with spatial coordinates to identify spatial domains, infer cell-type dynamics, and characterize gene expression patterns within tissues. Despite recent…

Quantitative Methods · Quantitative Biology 2026-03-25 Sean Cottrell , Guo-Wei Wei , Longxiu Huang

Accurate detection of cancer tissue regions (CTR) enables deeper analysis of the tumor microenvironment and offers crucial insights into treatment response. Traditional CTR detection methods, which typically rely on the rich cellular…

Computer Vision and Pattern Recognition · Computer Science 2026-03-09 Shuailin Xue , Jun Wan , Lihua Zhang , Wenwen Min

Spatial transcriptomics aims to connect high-resolution histology images with spatially resolved gene expression. To achieve better performance on downstream tasks such as gene expression prediction, large-scale pre-training is required to…

Computer Vision and Pattern Recognition · Computer Science 2025-09-23 Jiahe Qian , Yaoyu Fang , Ziqiao Weng , Xinkun Wang , Lee A. Cooper , Bo Zhou

Spatial transcriptomics (ST) is an emerging technology that enables researchers to investigate the molecular relationships underlying tissue morphology. However, acquiring ST data remains prohibitively expensive, and traditional fixed-grid…

Computer Vision and Pattern Recognition · Computer Science 2026-02-03 Junchao Zhu , Ruining Deng , Junlin Guo , Tianyuan Yao , Chongyu Qu , Juming Xiong , Siqi Lu , Zhengyi Lu , Yanfan Zhu , Marilyn Lionts , Yuechen Yang , Yalin Zheng , Yu Wang , Shilin Zhao , Haichun Yang , Yuankai Huo

Single source domain generalization (SDG) holds promise for more reliable and consistent image segmentation across real-world clinical settings particularly in the medical domain, where data privacy and acquisition cost constraints often…

Computer Vision and Pattern Recognition · Computer Science 2024-04-04 Shahina Kunhimon , Muzammal Naseer , Salman Khan , Fahad Shahbaz Khan

Spatial transcriptomics (ST) measures mRNA expression while preserving spatial organization, but multi-slice analysis faces two coupled difficulties: large non-rigid deformations across slices and inter-slice batch effects when alignment…

Computer Vision and Pattern Recognition · Computer Science 2026-04-15 Bonian Han , Cong Qi , Przemyslaw Musialski , Zhi Wei

The advancement of Spatial Transcriptomics (ST) has facilitated the spatially-aware profiling of gene expressions based on histopathology images. Although ST data offers valuable insights into the micro-environment of tumors, its…

Computer Vision and Pattern Recognition · Computer Science 2024-12-23 Hongyi Wang , Xiuju Du , Jing Liu , Shuyi Ouyang , Yen-Wei Chen , Lanfen Lin

Whole slide images (WSIs) enable weakly supervised prognostic modeling via multiple instance learning (MIL). Spatial transcriptomics (ST) preserves in situ gene expression, providing a spatial molecular context that complements morphology.…

Computer Vision and Pattern Recognition · Computer Science 2026-02-16 Lihe Liu , Xiaoxi Pan , Yinyin Yuan , Lulu Shang

Spatial transcriptomics enables genome-wide expression analysis within native tissue context, yet identifying spatial domains remains challenging due to complex gene-spatial interactions. Existing methods typically process spatial and…

Machine Learning · Computer Science 2025-12-19 Jianping Mei , Siqi Ai , Ye Yuan

Single-cell spatial transcriptomics (ST) offers a unique approach to measuring gene expression profiles and spatial cell locations simultaneously. However, most existing ST methods assume that cells in closer spatial proximity exhibit more…

Genomics · Quantitative Biology 2025-06-10 Xiongtao Xiao , Xiaofeng Chen , Feiyan Jiang , Songming Zhang , Wenming Cao , Cheng Tan , Zhangyang Gao , Zhongshan Li

Spatial transcriptomics (ST) is essential for understanding diseases and developing novel treatments. It measures gene expression of each fine-grained area (i.e., different windows) in the tissue slide with low throughput. This paper…

Computer Vision and Pattern Recognition · Computer Science 2022-11-01 Yan Yang , Md Zakir Hossain , Eric A Stone , Shafin Rahman