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Spatial Transcriptomics is a groundbreaking technology that integrates histology images with spatially resolved gene expression profiles. Among the various Spatial Transcriptomics techniques available, Visium has emerged as the most widely…

Computer Vision and Pattern Recognition · Computer Science 2025-09-05 Daniela Ruiz , Paula Cárdenas , Leonardo Manrique , Daniela Vega , Gabriel M. Mejia , Pablo Arbeláez

Spatially resolved transcriptomics (ST) measures gene expression along with the spatial coordinates of the measurements. The analysis of ST data involves significant computation complexity. In this work, we propose gene expression…

Genomics · Quantitative Biology 2022-05-24 Zhuoyan Xu , Kris Sankaran

Spatial Transcriptomics (ST) enables the measurement of gene expression while preserving spatial information, offering critical insights into tissue architecture and disease pathology. Recent developments have explored the use of…

Computer Vision and Pattern Recognition · Computer Science 2026-05-20 Hai Dang Nguyen , Nguyen Dang Huy Pham , The Minh Duc Nguyen , Dac Thai Nguyen , Hang Thi Nguyen , Duong M. Nguyen

Spatial Transcriptomics (ST) provides spatially resolved gene expression profiles within intact tissue architecture, enabling molecular analysis in histological context. However, the high cost, limited throughput, and restricted data…

Machine Learning · Computer Science 2026-03-31 Yaoyu Fang , Jiahe Qian , Xinkun Wang , Lee A. Cooper , Bo Zhou

The rapid development of spatial transcriptomics (ST) technologies is revolutionizing our understanding of the spatial organization of biological tissues. Current ST methods, categorized into next-generation sequencing-based (seq-based) and…

Machine Learning · Computer Science 2024-07-19 Xiaoyu Li , Fangfang Zhu , Wenwen Min

Spatial transcriptomics (ST) is essential for understanding diseases and developing novel treatments. It measures gene expression of each fine-grained area (i.e., different windows) in the tissue slide with low throughput. This paper…

Computer Vision and Pattern Recognition · Computer Science 2022-11-01 Yan Yang , Md Zakir Hossain , Eric A Stone , Shafin Rahman

This paper aims to predict gene expression from a histology slide image precisely. Such a slide image has a large resolution and sparsely distributed textures. These obstruct extracting and interpreting discriminative features from the…

Computer Vision and Pattern Recognition · Computer Science 2022-11-01 Yan Yang , LiYuan Pan , Liu Liu , Eric A Stone

Identifying disease-indicative genes is critical for deciphering disease mechanisms and has attracted significant interest in biomedical research. Spatial transcriptomics offers unprecedented insights for the detection of disease-specific…

Methodology · Statistics 2024-09-05 Qicheng Zhao , Qihuang Zhang

Popular technologies for generating spatially resolved transcriptomic data measure gene expression at the resolution of a "spot", i.e., a small tissue region 55 microns in diameter. Each spot can contain many cells of different types. In…

Applications · Statistics 2025-11-24 Hyun Jung Koo , Aaron J. Molstad

The spatial location of cells within tissues and organs is crucial for the manifestation of their specific functions.Spatial transcriptomics technology enables comprehensive measurement of the gene expression patterns in tissues while…

Quantitative Methods · Quantitative Biology 2024-07-12 Shuailin Xue , Fangfang Zhu , Changmiao Wang , Wenwen Min

Spatial Transcriptomics (ST) profiles thousands of gene expression values at discrete spots with precise coordinates on tissue sections, preserving spatial context essential for clinical and pathological studies. With rising sequencing…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Yishun Zhu , Jiaxin Qi , Jian Wang , Yuhua Zheng , Jianqiang Huang

Spatial Transcriptomics (ST) offers spatially resolved gene expression but remains costly. Predicting expression directly from widely available Hematoxylin and Eosin (H&E) stained images presents a cost-effective alternative. However, most…

Computer Vision and Pattern Recognition · Computer Science 2025-10-07 Jiarui Ouyang , Yihui Wang , Yihang Gao , Yingxue Xu , Shu Yang , Hao Chen

Spatial transcriptomics (ST) enables spatially resolved gene profiling but remains expensive and low-throughput, limiting large-cohort studies and routine clinical use. Predicting spatial gene expression from routine hematoxylin and eosin…

Computer Vision and Pattern Recognition · Computer Science 2026-04-24 Changfan Wang , Xinran Wang , Donghai Liu , Fei Su , Lulu Sun , Zhicheng Zhao , Zhu Meng

Deep learning-based nuclei segmentation and classification in pathology images typically rely on large-scale pixel-level manual annotations, which are costly and difficult to obtain across diverse tissues and staining conditions. To address…

Computer Vision and Pattern Recognition · Computer Science 2026-04-28 Kazuya Nishimura , Ryoma Bise , Haruka Hirose , Yasuhiro Kojima

Spatial transcriptomics (ST) reveals spatial heterogeneity of gene expression, yet its resolution is limited by current platforms. Recent methods enhance resolution via H&E-stained histology, but three major challenges persist: (1)…

Image and Video Processing · Electrical Eng. & Systems 2025-08-12 Xuepeng Liu , Zheng Jiang , Pinan Zhu , Hanyu Liu , Chao Li

The advancement of Spatial Transcriptomics (ST) has facilitated the spatially-aware profiling of gene expressions based on histopathology images. Although ST data offers valuable insights into the micro-environment of tumors, its…

Computer Vision and Pattern Recognition · Computer Science 2024-12-23 Hongyi Wang , Xiuju Du , Jing Liu , Shuyi Ouyang , Yen-Wei Chen , Lanfen Lin

Spatial transcriptomics (ST) has emerged as a powerful technology for bridging histology imaging with gene expression profiling. However, its application has been limited by low throughput and the need for specialized experimental…

Computer Vision and Pattern Recognition · Computer Science 2025-06-09 Tinglin Huang , Tianyu Liu , Mehrtash Babadi , Wengong Jin , Rex Ying

Spatial transcriptomics (ST) measures gene expression at a set of spatial locations in a tissue. Communities of nearby cells that express similar genes form \textit{spatial domains}. Specialized ST clustering algorithms have been developed…

Quantitative Methods · Quantitative Biology 2025-11-12 Perry Beamer , Zixuan Cang

The advancement of Spatial Transcriptomics (ST) has facilitated the spatially-aware profiling of gene expressions based on histopathology images. Although ST data offers valuable insights into the micro-environment of tumors, its…

Computer Vision and Pattern Recognition · Computer Science 2024-01-25 Hongyi Wang , Xiuju Du , Jing Liu , Shuyi Ouyang , Yen-Wei Chen , Lanfen Lin

Single-cell spatial transcriptomics (ST) offers a unique approach to measuring gene expression profiles and spatial cell locations simultaneously. However, most existing ST methods assume that cells in closer spatial proximity exhibit more…

Genomics · Quantitative Biology 2025-06-10 Xiongtao Xiao , Xiaofeng Chen , Feiyan Jiang , Songming Zhang , Wenming Cao , Cheng Tan , Zhangyang Gao , Zhongshan Li