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Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…

Optics · Physics 2025-02-20 Wei Wang , Zengxin Huang , Yilin Wang , Hangfeng Li , Pakorn Kanchanawong

We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…

Fluorescence microscopy is essential in biological and medical research, providing critical insights into cellular structures. However, limited by optical diffraction and background noise, a substantial amount of hidden information is still…

Biological Physics · Physics 2026-04-14 Xiaofeng Zhang , Yongsheng Huang , Jielong Yang , Zhili Wang , Si Chen , Linbo Liu , Xin Ge

Nanophotonics offers a promising range of applications spanning from the development of efficient solar cells to quantum communications and biosensing. However, the ability to efficiently couple fluorescent emitters with nanostructured…

Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

Biomolecules · Quantitative Biology 2015-04-15 Mark Leake

High-resolution fMRI provides a window into the brain's mesoscale organization. Yet, higher spatial resolution increases scan times, to compensate for the low signal and contrast-to-noise ratio. This work introduces a deep learning-based 3D…

Image and Video Processing · Electrical Eng. & Systems 2024-03-20 Hongwei Bran Li , Matthew S. Rosen , Shahin Nasr , Juan Eugenio Iglesias

Event-based image sensors provide microsecond temporal resolution but lack spectral discrimination, whereas diffractive spectral imagers encode wavelength information at conventional frame rates. We introduce a fluorescence microscopy…

Optics · Physics 2026-03-20 Richard G. Baird , Boyden Myers , Erik M. Jorgensen , Rajesh Menon

The resolution of optical imaging devices is ultimately limited by the diffraction of light. To circumvent this limit, modern super-resolution microscopy techniques employ active interaction with the object by exploiting its optical…

Optics · Physics 2022-01-05 A. A. Pushkina , G. Maltese , J. I. Costa-Filho , P. Patel , A. I. Lvovsky

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

Optics · Physics 2024-03-26 Tingting Wu , Matthew D. Lew

The color X-ray camera SLcam(R) is a full-field, single photon detector providing scanning free, energy and spatially resolved X-ray imaging. Spatial resolution is achieved with the use of polycapillary optics guiding X-ray photons from…

Recently, imaging by microspheres and dielectric particle-lenses emerged as a simple solution to obtaining super-resolution images of nanoscale devices and structures. Calibrated resolution of ~{\lambda}/6 - {\lambda}/8 has been…

Optics · Physics 2021-08-26 Zengbo Wang , Boris Luk'yanchuk

Low quantum yields of near infrared (NIR) fluorophores have limited their capabilities as imaging probes in a transparent, low background imaging window. Here for the first time we reported near-infrared fluorescence enhance (NIR-FE) cell…

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

The emergence of far-field super-resolution microscopy has rejuvenated the possibility for nanoscale imaging. Approaches to far-field super-resolution that utilize point scanning often depends on spatially reducing the size of the focused…

Optics · Physics 2022-06-17 Ning Xu , Guoxuan Liu , Qiaofeng Tan

Single-image super-resolution (SISR) is a canonical problem with diverse applications. Leading methods like SRGAN produce images that contain various artifacts, such as high-frequency noise, hallucinated colours and shape distortions, which…

Machine Learning · Computer Science 2018-10-03 Ke Li , Shichong Peng , Jitendra Malik

Light-sheet microscopes must compromise between field of view, optical sectioning, resolution, and detection efficiency. High-numerical-aperture (NA) detection objective lenses provide high resolution but their narrow depth of field fails…

3D super-resolution fluorescence microscopy typically requires sophisticated setups, sample preparation, or long measurements. A notable exception, SOFI, only requires recording a sequence of frames and no hardware modifications whatsoever…

Optics · Physics 2024-03-01 Pawel Szczypkowski , Monika Pawlowska , Radek Lapkiewicz

In deep tissue photoacoustic imaging the spatial resolution is inherently limited by the acoustic wavelength. We present an approach for surpassing the acoustic diffraction limit by exploiting temporal fluctuations in the sample absorption…

Optics · Physics 2017-05-31 Thomas Chaigne , Bastien Arnal , Sergey Vilov , Emmanuel Bossy , Ori Katz

Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. Currently, this optical-sectioning technique is limited by image…