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Volumetric imaging by fluorescence microscopy is often limited by anisotropic spatial resolution from inferior axial resolution compared to the lateral resolution. To address this problem, here we present a deep-learning-enabled…

Computer Vision and Pattern Recognition · Computer Science 2022-07-06 Hyoungjun Park , Myeongsu Na , Bumju Kim , Soohyun Park , Ki Hean Kim , Sunghoe Chang , Jong Chul Ye

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

Fluorescence microscopy is a key driver to promote discoveries of biomedical research. However, with the limitation of microscope hardware and characteristics of the observed samples, the fluorescence microscopy images are susceptible to…

Image and Video Processing · Electrical Eng. & Systems 2022-09-15 Xuanyu Tian , Qing Wu , Hongjiang Wei , Yuyao Zhang

Photoacoustic tomography (PAT) is a promising imaging technique that can visualize the distribution of chromophores within biological tissue. However, the accuracy of PAT imaging is compromised by light fluence (LF), which hinders the…

Image and Video Processing · Electrical Eng. & Systems 2023-12-11 Zhaoyong Liang , Shuangyang Zhang , Zhichao Liang , Zhongxin Mo , Xiaoming Zhang , Yutian Zhong , Wufan Chen , Li Qi

We consider the problem of high-dimensional light field reconstruction and develop a learning-based framework for spatial and angular super-resolution. Many current approaches either require disparity clues or restore the spatial and…

Image and Video Processing · Electrical Eng. & Systems 2020-09-18 Nan Meng , Hayden K. -H. So , Xing Sun , Edmund Y. Lam

Due to image blurring image deconvolution is often used for studying biological structures in fluorescence microscopy. Fluorescence microscopy image volumes inherently suffer from intensity inhomogeneity, blur, and are corrupted by various…

Computer Vision and Pattern Recognition · Computer Science 2019-07-15 Soonam Lee , Shuo Han , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

This paper introduces a novel framework for image quality transfer based on conditional flow matching (CFM). Unlike conventional generative models that rely on iterative sampling or adversarial objectives, CFM learns a continuous flow…

Computer Vision and Pattern Recognition · Computer Science 2025-10-15 Huu Tien Nguyen , Ahmed Karam Eldaly

Light microscopy is a widespread and inexpensive imaging technique facilitating biomedical discovery and diagnostics. However, light diffraction barrier and imperfections in optics limit the level of detail of the acquired images. The…

Quantitative Methods · Quantitative Biology 2026-03-24 Rui Li , Gabriel della Maggiora , Vardan Andriasyan , Anthony Petkidis , Artsemi Yushkevich , Mikhail Kudryashev , Artur Yakimovich

Ultrasound Localization Microscopy can resolve the microvascular bed down to a few micrometers. To achieve such performance microbubble contrast agents must perfuse the entire microvascular network. Microbubbles are then located…

Computer Vision and Pattern Recognition · Computer Science 2023-10-13 Léo Milecki , Jonathan Porée , Hatim Belgharbi , Chloé Bourquin , Rafat Damseh , Patrick Delafontaine-Martel , Frédéric Lesage , Maxime Gasse , Jean Provost

Today the gold standard for in vivo imaging through scattering tissue is the point-scanning two-photon microscope (PSTPM). Especially in neuroscience, PSTPM is widely used for deep-tissue imaging in the brain. However, due to sequential…

Image and Video Processing · Electrical Eng. & Systems 2020-01-03 Zhun Wei , Josiah R. Boivin , Yi Xue , Xudong Chen , Peter T. C. So , Elly Nedivi , Dushan N. Wadduwage

A densely-sampled light field (LF) is highly desirable in various applications, such as 3-D reconstruction, post-capture refocusing and virtual reality. However, it is costly to acquire such data. Although many computational methods have…

Image and Video Processing · Electrical Eng. & Systems 2020-09-29 Jing Jin , Junhui Hou , Jie Chen , Huanqiang Zeng , Sam Kwong , Jingyi Yu

Fluorescence microscopy plays an important role in biomedical research. The depth-variant point spread function (PSF) of a fluorescence microscope produces low-quality images especially in the out-of-focus regions of thick specimens.…

Image and Video Processing · Electrical Eng. & Systems 2019-07-09 Da He , De Cai , Jiasheng Zhou , Jiajia Luo , Sung-Liang Chen

Light field cameras have a wide range of uses due to their ability to simultaneously record light intensity and direction. The angular resolution of light fields is important for downstream tasks such as depth estimation, yet is often…

Computer Vision and Pattern Recognition · Computer Science 2023-11-15 Langqing Shi , Ping Zhou

Reconstructing accurate implicit surface representations from point clouds remains a challenging task, particularly when data is captured using low-quality scanning devices. These point clouds often contain substantial noise, leading to…

Computer Vision and Pattern Recognition · Computer Science 2025-09-30 Tengkai Wang , Weihao Li , Ruikai Cui , Shi Qiu , Nick Barnes

A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing…

We present a deep neural network to reduce coherent noise in three-dimensional quantitative phase imaging. Inspired by the cycle generative adversarial network, the denoising network was trained to learn a transform between two image…

Multimode fibers (MMFs) have the potential to carry complex images for endoscopy and related applications, but decoding the complex speckle patterns produced by mode-mixing and modal dispersion in MMFs is a serious challenge. Several groups…

Image and Video Processing · Electrical Eng. & Systems 2020-09-02 Changyan Zhu , Eng Aik Chan , You Wang , Weina Peng , Ruixiang Guo , Baile Zhang , Cesare Soci , Yidong Chong

Compared to light-field microscopy (LFM), which enables high-speed volumetric imaging but suffers from non-uniform spatial sampling, Fourier light-field microscopy (FLFM) introduces sub-aperture division at the pupil plane, thereby ensuring…

Image and Video Processing · Electrical Eng. & Systems 2026-05-28 Chenyu Xu , Zhouyu Jin , Chengkang Shen , Hao Zhu , Zhan Ma , Bo Xiong , You Zhou , Xun Cao , Ning Gu

Restoring a sharp light field image from its blurry input has become essential due to the increasing popularity of parallax-based image processing. State-of-the-art blind light field deblurring methods suffer from several issues such as…

Computer Vision and Pattern Recognition · Computer Science 2019-12-06 Jonathan Samuel Lumentut , Tae Hyun Kim , Ravi Ramamoorthi , In Kyu Park