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Deciphering how DNA sequence encodes gene regulation remains a central challenge in biology. Advances in machine learning and functional genomics have enabled sequence-to-function (seq2func) models that predict molecular regulatory readouts…

Genomics · Quantitative Biology 2026-02-03 Masayuki Nagai , Alan E. Murphy , Kaeli Rizzo , Peter K. Koo

Gene regulation is a complex process involving the role of several genomic elements which work in concert to drive spatio-temporal expression. The experimental characterization of gene regulatory elements is a very complex and…

Genomics · Quantitative Biology 2007-10-11 Arvind Rao , Alfred O. Hero , David J. States , James Douglas Engel

It is tempting to believe that we now own the genome. The ability to read and re-write it at will has ushered in a stunning period in the history of science. Nonetheless, there is an Achilles heel exposed by all of the genomic data that has…

Gene regulation in higher eukaryotes involves a complex interplay between the gene proximal promoter and distal genomic elements (such as enhancers) which work in concert to drive spatio-temporal expression. The experimental…

Genomics · Quantitative Biology 2008-03-24 Arvind Rao , Alfred O. Hero , David J. States , James Douglas Engel

Gene regulation in eukaryotes is mainly effected through transcription factors binding to rather short recognition motifs generally located upstream of the coding region. We present a novel computational method to identify regulatory…

Biological Physics · Physics 2007-05-23 M. Caselle , F. Di Cunto , P. Provero

We present a novel classification-based method for learning to predict gene regulatory response. Our approach is motivated by the hypothesis that in simple organisms such as Saccharomyces cerevisiae, we can learn a decision rule for…

Quantitative Methods · Quantitative Biology 2007-05-23 Manuel Middendorf , Anshul Kundaje , Chris Wiggins , Yoav Freund , Christina Leslie

Developing models with high interpretability and even deriving formulas to quantify relationships between biological data is an emerging need. We propose here a framework for ab initio derivation of sequence motifs and linear formula using…

Quantitative Methods · Quantitative Biology 2022-08-23 Chengyu Liu , Wei Wang

Predicting how genetic variation affects phenotypic outcomes at the organismal, cellular, and molecular levels requires deciphering the cis-regulatory code, the sequence rules by which non-coding regions regulate genes. In this perspective,…

Gene expression-based heterogeneity analysis has been extensively conducted. In recent studies, it has been shown that network-based analysis, which takes a system perspective and accommodates the interconnections among genes, can be more…

Methodology · Statistics 2023-08-09 Rong Li , Qingzhao Zhang , Shuangge Ma

Gene regulation involves a hierarchy of events that extend from specific protein-DNA interactions to the combinatorial assembly of nucleoprotein complexes. The effects of DNA sequence on these processes have typically been studied based…

Molecular Networks · Quantitative Biology 2015-05-20 Jose M. G. Vilar

A transversal topic of my research has been the development and application of computational methods for DNA sequence analysis. The methods I have been developing aim at improving our understanding of the regulation processes happening in…

Genomics · Quantitative Biology 2014-09-24 Valentina Boeva

Gene regulation in Eukaryotes is mainly effected through transcription factors binding to rather short recognition motifs generally located upstream of the coding region. We present a novel computational method to identify regulatory…

Disordered Systems and Neural Networks · Physics 2007-05-23 M. Caselle , F. Di Cunto , P. Provero

Modelling gene regulatory networks not only requires a thorough understanding of the biological system depicted but also the ability to accurately represent this system from a mathematical perspective. Throughout this chapter, we aim to…

Quantitative Methods · Quantitative Biology 2018-05-04 Olivia Angelin-Bonnet , Patrick J. Biggs , Matthieu Vignes

The discovery of motifs underlying gene expression is a challenging one. Some of these motifs are known transcription factors, but sequence inspection often provides valuable clues, even discovery of novel motifs with uncharacterized…

Genomics · Quantitative Biology 2007-05-23 Arvind Rao , Alfred O. Hero , David J. States , James Douglas Engel

Gene regulation is a dynamic process that connects genotype and phenotype. Given the difficulty of physically mapping mammalian gene circuitry, we require new computational methods to learn regulatory rules. Natural language is a valuable…

Quantitative Methods · Quantitative Biology 2022-10-27 William Connell , Umair Khan , Michael J. Keiser

Gene regulation is an important fundamental biological process. The regulation of gene expression is managed through a variety of methods including epigenetic processes (e.g., DNA methylation). Understanding the role of epigenetic changes…

Molecular Networks · Quantitative Biology 2021-06-01 James Brunner , Jacob Kim , Timothy Downing , Eric Mjolsness , Kord M. Kober

Over the past decade, neural networks have been successful at making predictions from biological sequences, especially in the context of regulatory genomics. As in other fields of deep learning, tools have been devised to extract features…

Genomics · Quantitative Biology 2022-12-27 Antoine Villié , Philippe Veber , Yohann de Castro , Laurent Jacob

We present a novel classification-based algorithm called GeneClass for learning to predict gene regulatory response. Our approach is motivated by the hypothesis that in simple organisms such as Saccharomyces cerevisiae, we can learn a…

Quantitative Methods · Quantitative Biology 2007-05-23 Manuel Middendorf , Anshul Kundaje , Chris Wiggins , Yoav Freund , Christina Leslie

Genetic differences between individuals associated to quantitative phenotypic traits, including disease states, are usually found in non-coding genomic regions. These genetic variants are often also associated to differences in expression…

Molecular Networks · Quantitative Biology 2016-11-02 Lingfei Wang , Tom Michoel

Due to recent breakthroughs in state-of-the-art DNA sequencing technology, genomics data sets have become ubiquitous. The emergence of large-scale data sets provides great opportunities for better understanding of genomics, especially gene…

Genomics · Quantitative Biology 2020-12-18 Wei Cheng , Ghulam Murtaza , Aaron Wang
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