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A key challenge in systems biology is the elucidation of the underlying principles, or fundamental laws, which determine the cellular phenotype. Understanding how these fundamental principles are altered in diseases like cancer is important…

Molecular Networks · Quantitative Biology 2014-08-06 Andrew Teschendorff , Peter Sollich , Reimer Kuehn

Joint analysis of multi-omic single-cell data across cohorts has significantly enhanced the comprehensive analysis of cellular processes. However, most of the existing approaches for this purpose require access to samples with complete…

Machine Learning · Computer Science 2024-05-21 Marianne Arriola , Weishen Pan , Manqi Zhou , Qiannan Zhang , Chang Su , Fei Wang

Single-molecule biophysics has transformed our understanding of the fundamental molecular processes involved in living biological systems, but also of the fascinating physics of life. Far more exotic than a collection of exemplars of soft…

Biological Physics · Physics 2017-09-08 Helen Miller , Zhaokun Zhou , Jack Shepherd , Adam J. M. Wollman , Mark C. Leake

In immunological studies, the characterization of small, functionally distinct cell subsets from blood and tissue is crucial to decipher system level biological changes. An increasing number of studies rely on assays that provide…

Early detection of colorectal polyps is of utmost importance for their treatment and for colorectal cancer prevention. Computer vision techniques have the potential to aid professionals in the diagnosis stage, where colonoscopies are…

Computer Vision and Pattern Recognition · Computer Science 2023-07-25 Enric Moreu , Eric Arazo , Kevin McGuinness , Noel E. O'Connor

Recent advancements in single-cell genomics necessitate precision in gene panel selection to interpret complex biological data effectively. Those methods aim to streamline the analysis of scRNA-seq data by focusing on the most informative…

Artificial Intelligence · Computer Science 2024-06-12 Weiliang Zhang , Zhen Meng , Dongjie Wang , Min Wu , Kunpeng Liu , Yuanchun Zhou , Meng Xiao

Single-cell technologies offer insights into molecular feature distributions, but comparing them poses challenges. We propose a kernel-testing framework for non-linear cell-wise distribution comparison, analyzing gene expression and…

Single-cell transcriptomics and proteomics have become a great source for data-driven insights into biology, enabling the use of advanced deep learning methods to understand cellular heterogeneity and gene expression at the single-cell…

Genomics · Quantitative Biology 2025-12-15 Hiren Madhu , João Felipe Rocha , Tinglin Huang , Siddharth Viswanath , Smita Krishnaswamy , Rex Ying

Mass spectrometry provides a high-throughput way to identify proteins in biological samples. In a typical experiment, proteins in a sample are first broken into their constituent peptides. The resulting mixture of peptides is then subjected…

Applications · Statistics 2010-11-10 Qunhua Li , Michael J. MacCoss , Matthew Stephens

Flow cytometry (FCM) is the standard multi-parameter assay for measuring single cell phenotype and functionality. It is commonly used for quantifying the relative frequencies of cell subsets in blood and disaggregated tissues. A typical…

Applications · Statistics 2020-09-01 Shai Gorsky , Cliburn Chan , Li Ma

Single-cell RNA sequencing (scRNA-seq) has revolutionized our ability to analyze gene expression at the cellular level. By providing data on gene expression for each individual cell, scRNA-seq generates large datasets with thousands of…

Computational Complexity · Computer Science 2025-02-11 Md Romizul Islam , Swakkhar Shatabda

Cellular signaling is essential in information processing and decision making. Therefore, a variety of experimental approaches have been developed to study signaling on bulk and single-cell level. Single-cell measurements of signaling…

Quantitative Methods · Quantitative Biology 2019-04-18 Carolin Loos , Jan Hasenauer

Single-cell RNA sequencing (scRNA-seq) is essential for unraveling cellular heterogeneity and diversity, offering invaluable insights for bioinformatics advancements. Despite its potential, traditional clustering methods in scRNA-seq data…

Machine Learning · Computer Science 2025-10-01 Ping Xu , Zhiyuan Ning , Meng Xiao , Guihai Feng , Xin Li , Yuanchun Zhou , Pengfei Wang

Flow cytometry is a cornerstone technique in medical and biological research, providing crucial information about cell size and granularity through forward scatter (FSC) and side scatter (SSC) signals. Despite its widespread use, the…

Optics · Physics 2024-08-29 Jaepil Jo , Herve Hugonnet , Mahn Jae Lee , YongKeun Park

Single cell analysis of skeletal muscle (SM) tissue is a fundamental tool for understanding many neuromuscular disorders. For this analysis to be reliable and reproducible, identification of individual fibres within microscopy images…

Computer Vision and Pattern Recognition · Computer Science 2023-11-21 Atif Khan , Conor Lawless , Amy Vincent , Charlotte Warren , Valeria Di Leo , Tiago Gomes , A. Stephen McGough

Spectral clustering is a popular method for effectively clustering nonlinearly separable data. However, computational limitations, memory requirements, and the inability to perform incremental learning challenge its widespread application.…

Machine Learning · Computer Science 2023-11-15 Jo-Chun Chen , Hung-Hsuan Chen

Xenium, a new spatial transcriptomics platform, enables subcellular-resolution profiling of complex tumor tissues. Despite the rich morphological information in histology images, extracting robust cell-level features and integrating them…

Computer Vision and Pattern Recognition · Computer Science 2025-08-15 Paul H. Acosta , Pingjun Chen , Simon P. Castillo , Maria Esther Salvatierra , Yinyin Yuan , Xiaoxi Pan

Despite the significant increase in computational power, molecular modeling of protein structure using classical all-atom approaches remains inefficient, at least for most of the protein targets in the focus of biomedical research. Perhaps…

Biomolecules · Quantitative Biology 2016-11-01 Sebastian Kmiecik , Andrzej Kolinski

High-dimensional single-cell data poses significant challenges in identifying underlying biological patterns due to the complexity and heterogeneity of cellular states. We propose a comprehensive gene-cell dependency visualization via…

Machine Learning · Computer Science 2024-07-25 Shang-Jung Wen , Jia-Ming Chang , Fang Yu

Breast cancer's complexity and variability pose significant challenges in understanding its progression and guiding effective treatment. This study aims to integrate protein sequence data with expression levels to improve the molecular…

Biomolecules · Quantitative Biology 2025-10-31 Hossein Sholehrasa , Majid Jaberi-Douraki