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Cryo-electron microscopy (cryo-EM) has achieved near-atomic level resolution of biomolecules by reconstructing 2D micrographs. However, the resolution and accuracy of the reconstructed particles are significantly reduced due to the…

Computer Vision and Pattern Recognition · Computer Science 2024-01-03 Jing Zhang , Tengfei Zhao , ShiYu Hu , Xin Zhao

Cryo-electron microscopy (EM) single particle reconstruction is an entirely general technique for 3D structure determination of macromolecular complexes. However, because the images are taken at low electron dose, it is extremely hard to…

Computer Vision and Pattern Recognition · Computer Science 2019-04-17 Yifeng Fan , Zhizhen Zhao

In recent years, convolutional neural networks have demonstrated promising performance in a variety of medical image segmentation tasks. However, when a trained segmentation model is deployed into the real clinical world, the model may not…

Image and Video Processing · Electrical Eng. & Systems 2020-12-24 Shuo Wang , Giacomo Tarroni , Chen Qin , Yuanhan Mo , Chengliang Dai , Chen Chen , Ben Glocker , Yike Guo , Daniel Rueckert , Wenjia Bai

Medical ultrasound image segmentation presents a formidable challenge in the realm of computer vision. Traditional approaches rely on Convolutional Neural Networks (CNNs) and Transformer-based methods to address the intricacies of medical…

Computer Vision and Pattern Recognition · Computer Science 2025-08-20 Weixin Xu , Ziliang Wang

Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

Quantitative Methods · Quantitative Biology 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

Neural Radiance Fields (NeRF) are an advanced technology that creates highly realistic images by learning about scenes through a neural network model. However, NeRF often encounters issues when there are not enough images to work with,…

Computer Vision and Pattern Recognition · Computer Science 2024-07-09 Jiawei Guo , HungChyun Chou , Ning Ding

High-fidelity electron microscopy simulations required for quantitative crystal structure refinements face a fundamental challenge: while physical interactions are well-described theoretically, real-world experimental effects are…

Learning-based edge detection models trained with cross-entropy loss often suffer from thick edge predictions, which deviate from the crisp, single-pixel annotations typically provided by humans. While previous approaches to achieving crisp…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Jiaxin Cheng , Yue Wu , Yicong Zhou

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

Computer Vision and Pattern Recognition · Computer Science 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

In cryo-electron microscopy (EM), molecular structures are determined from large numbers of projection images of individual particles. To harness the full power of this single-molecule information, we use the Bayesian inference of EM…

Biomolecules · Quantitative Biology 2018-01-17 Pilar Cossio , David Rohr , Fabio Baruffa , Markus Rampp , Volker Lindenstruth , Gerhard Hummer

Weakly supervised semantic segmentation (WSSS) with image-level labels is a challenging task. Mainstream approaches follow a multi-stage framework and suffer from high training costs. In this paper, we explore the potential of Contrastive…

Computer Vision and Pattern Recognition · Computer Science 2023-03-24 Yuqi Lin , Minghao Chen , Wenxiao Wang , Boxi Wu , Ke Li , Binbin Lin , Haifeng Liu , Xiaofei He

Simulation-based inference provides a powerful framework for cryo-electron microscopy, employing neural networks in methods like CryoSBI to infer biomolecular conformations via learned latent representations. This latent space represents a…

Quantitative Methods · Quantitative Biology 2025-09-05 Luke Evans , Octavian-Vlad Murad , Lars Dingeldein , Pilar Cossio , Roberto Covino , Marina Meila

Single particle cryo-electron microscopy (EM) is an increasingly popular method for determining the 3-D structure of macromolecules from noisy 2-D images of single macromolecules whose orientations and positions are random and unknown. One…

Computer Vision and Pattern Recognition · Computer Science 2017-04-11 Roy R. Lederman , Amit Singer

Semantic segmentation is one of the key tasks in computer vision, which is to assign a category label to each pixel in an image. Despite significant progress achieved recently, most existing methods still suffer from two challenging issues:…

Computer Vision and Pattern Recognition · Computer Science 2022-09-08 Jianlong Yuan , Zelu Deng , Shu Wang , Zhenbo Luo

Studying the growth and metabolism of microbes provides critical insights into their evolutionary adaptations to harsh environments, which are essential for microbial research and biotechnology applications. In this study, we developed an…

Image and Video Processing · Electrical Eng. & Systems 2025-05-06 Shuang Zhang , Carleton Coffin , Karyn L. Rogers , Catherine Ann Royer , Ge Wang

Cryogenic electron microscopy (cryo-EM) is an invaluable technique for determining high-resolution three-dimensional structures of biological macromolecules using transmission particle images. The inherent symmetry in these macromolecules…

Optimization and Control · Mathematics 2024-11-06 Qi Zhang , Chenglong Bao , Hai Lin , Mingxu Hu

The increased availability and usage of modern medical imaging induced a strong need for automatic medical image segmentation. Still, current image segmentation platforms do not provide the required functionalities for plain setup of…

Image and Video Processing · Electrical Eng. & Systems 2022-04-14 Dominik Müller , Frank Kramer

Training of semantic segmentation models for material analysis requires micrographs and their corresponding masks. It is quite unlikely that perfect masks will be drawn, especially at the edges of objects, and sometimes the amount of data…

Computer Vision and Pattern Recognition · Computer Science 2024-08-02 Matias Oscar Volman Stern , Dominic Hohs , Andreas Jansche , Timo Bernthaler , Gerhard Schneider

Remote sensing semantic segmentation aims to assign automatically each pixel on aerial images with specific label. In this letter, we proposed a new module, called improved-flow warp module (IFWM), to adjust semantic feature maps across…

Computer Vision and Pattern Recognition · Computer Science 2022-05-10 Yinjie Zhang , Yi Liu , Wei Guo

Accurate delineation of individual cells in microscopy videos is essential for studying cellular dynamics, yet separating touching or overlapping instances remains a persistent challenge. Although foundation-model for segmentation such as…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Thomas Mendelson , Joshua Francois , Galit Lahav , Tammy Riklin-Raviv