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Cryogenic Electron Tomography (CryoET) is a useful imaging technology in structural biology that is hindered by its need for manual annotations, especially in particle picking. Recent works have endeavored to remedy this issue with few-shot…

Quantitative Methods · Quantitative Biology 2024-07-10 Yizhou Zhao , Hengwei Bian , Michael Mu , Mostofa R. Uddin , Zhenyang Li , Xiang Li , Tianyang Wang , Min Xu

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

State-of-the-art methods for semantic segmentation of images involve computationally intensive neural network architectures. Most of these methods are not adaptable to high-resolution image segmentation due to memory and other computational…

Computer Vision and Pattern Recognition · Computer Science 2023-10-27 Siddharth Saravanan , Aditya Challa , Sravan Danda

Semantic segmentation is a fundamental task in multimedia processing, which can be used for analyzing, understanding, editing contents of images and videos, among others. To accelerate the analysis of multimedia data, existing segmentation…

Computer Vision and Pattern Recognition · Computer Science 2024-12-13 Zhiyan Wang , Deyin Liu , Lin Yuanbo Wu , Song Wang , Xin Guo , Lin Qi

In the field of integrated circuit manufacturing, the detection and classification of nanoscale wafer defects are critical for subsequent root cause analysis and yield enhancement. The complex background patterns observed in scanning…

Computer Vision and Pattern Recognition · Computer Science 2025-02-24 Qian Jin , Yuqi Jiang , Xudong Lu , Yumeng Liu , Yining Chen , Dawei Gao , Qi Sun , Cheng Zhuo

We present a fully modular and scalable software pipeline for processing electron microscope (EM) images of brain slices into 3D visualization of individual neurons and demonstrate an end-to-end segmentation of a large EM volume using a…

Distributed, Parallel, and Cluster Computing · Computer Science 2020-11-20 Rafael Vescovi , Hanyu Li , Jeffery Kinnison , Murat Keceli , Misha Salim , Narayanan Kasthuri , Thomas D. Uram , Nicola Ferrier

Cryo-electron microscopy (cryo-EM) enables the atomic-resolution visualization of biomolecules; however, modern direct detectors generate data volumes that far exceed the available storage and transfer bandwidth, thereby constraining…

Image and Video Processing · Electrical Eng. & Systems 2026-03-31 Zain Shabeeb , Daniel Saeedi , Darin Tsui , Vida Jamali , Amirali Aghazadeh

Foreground components in the Cosmic Microwave Background (CMB) are sparse in a needlet representation, due to their specific morphological features (anisotropy, non-Gaussianity). This leads to the possibility of applying needlet…

Cosmology and Nongalactic Astrophysics · Physics 2021-12-01 F. Oppizzi , A. Renzi , M. Liguori , F. K. Hansen , D. Marinucci , C. Baccigalupi , D. Bertacca , D. Poletti

We revisit the topic of common lines between projection images in single particle cryo-electron microscopy (cryo-EM). We derive a novel low-rank constraint on a certain $2n \times n$ matrix storing properly-scaled basis vectors for the…

Optimization and Control · Mathematics 2024-06-10 Tommi Muller , Adriana L. Duncan , Eric J. Verbeke , Joe Kileel

The cryo-electron microscope (cryo-EM) is increasingly popular these years. It helps to uncover the biological structures and functions of macromolecules. In this paper, we address image denoising problem in cryo-EM. Denoising the cryo-EM…

Computation · Statistics 2018-10-26 Yin Xian , Hanlin Gu , Wei Wang , Xuhui Huang , Yuan Yao , Yang Wang , Jian-Feng Cai

The fully connected conditional random field (CRF) with Gaussian pairwise potentials has proven popular and effective for multi-class semantic segmentation. While the energy of a dense CRF can be minimized accurately using a linear…

Computer Vision and Pattern Recognition · Computer Science 2017-02-15 Thalaiyasingam Ajanthan , Alban Desmaison , Rudy Bunel , Mathieu Salzmann , Philip H. S. Torr , M. Pawan Kumar

Accurate characterization of carbon nanotube morphologies in electron microscopy images is vital for exposure assessment and toxicological studies, yet current workflows rely on slow, subjective manual segmentation. This work presents a…

Computer Vision and Pattern Recognition · Computer Science 2026-02-06 Sanjay Pradeep , Chen Wang , Matthew M. Dahm , Jeff D. Eldredge , Candace S. J. Tsai

Determining the three-dimensional structure of proteins and protein complexes at atomic resolution is a fundamental task in structural biology. Over the last decade, remarkable progress has been made using "single particle" cryo-electron…

Numerical Analysis · Mathematics 2017-06-05 Alex Barnett , Leslie Greengard , Andras Pataki , Marina Spivak

When it comes to clinical images, automatic segmentation has a wide variety of applications and a considerable diversity of input domains, such as different types of Magnetic Resonance Images (MRIs) and Computerized Tomography (CT) scans.…

Image and Video Processing · Electrical Eng. & Systems 2024-02-28 Matteo Bastico , David Ryckelynck , Laurent Corté , Yannick Tillier , Etienne Decencière

We present LS-CRF, a new method for very efficient large-scale training of Conditional Random Fields (CRFs). It is inspired by existing closed-form expressions for the maximum likelihood parameters of a generative graphical model with tree…

Machine Learning · Computer Science 2014-03-28 Alexander Kolesnikov , Matthieu Guillaumin , Vittorio Ferrari , Christoph H. Lampert

Automated surface segmentation is important and challenging in many medical image analysis applications. Recent deep learning based methods have been developed for various object segmentation tasks. Most of them are a classification based…

Computer Vision and Pattern Recognition · Computer Science 2019-09-25 Leixin Zhou , Zisha Zhong , Abhay Shah , Bensheng Qiu , John Buatti , Xiaodong Wu

In single-particle cryo-electron microscopy (cryo-EM), the efficient determination of orientation parameters for 2D projection images poses a significant challenge yet is crucial for reconstructing 3D structures. This task is complicated by…

Image and Video Processing · Electrical Eng. & Systems 2025-09-30 Szu-Chi Chung

Single-particle cryo-Electron Microscopy (EM) has become a popular technique for determining the structure of challenging biomolecules that are inaccessible to other technologies. Recent advances in automation, both in data collection and…

Computer Vision and Pattern Recognition · Computer Science 2021-05-31 Ye Zhou , Amit Moscovich , Tamir Bendory , Alberto Bartesaghi

Achieving high-quality semantic segmentation predictions using only image-level labels enables a new level of real-world applicability. Although state-of-the-art networks deliver reliable predictions, the amount of handcrafted pixel-wise…

Computer Vision and Pattern Recognition · Computer Science 2023-08-17 Erik Ostrowski , Bharath Srinivas Prabakaran , Muhammad Shafique

Cryo-electron microscopy (cryo-EM) is an experimental technique for protein structure determination that images an ensemble of macromolecules in near-physiological contexts. While recent advances enable the reconstruction of dynamic…