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Directed evolution as a widely-used engineering strategy faces obstacles in finding desired mutants from the massive size of candidate modifications. While deep learning methods learn protein contexts to establish feasible searching space,…

Quantitative Methods · Quantitative Biology 2023-04-18 Bingxin Zhou , Outongyi Lv , Kai Yi , Xinye Xiong , Pan Tan , Liang Hong , Yu Guang Wang

Directed evolution is a versatile technique in protein engineering that mimics the process of natural selection by iteratively alternating between mutagenesis and screening in order to search for sequences that optimize a given property of…

Biomolecules · Quantitative Biology 2024-05-02 Lixue Cheng , Ziyi Yang , Changyu Hsieh , Benben Liao , Shengyu Zhang

A basic question of protein structural studies is to which extent mutations affect the stability. This question may be addressed starting from sequence and/or from structure. In proteomics and genomics studies prediction of protein…

Biomolecules · Quantitative Biology 2007-06-13 Emidio Capriotti , Piero Fariselli , Ivan Rossi , Rita Casadio

Despite recent strides made by AI in image processing, the issue of mixed exposure, pivotal in many real-world scenarios like surveillance and photography, remains inadequately addressed. Traditional image enhancement techniques and current…

Computer Vision and Pattern Recognition · Computer Science 2026-01-27 Eashan Adhikarla , Kai Zhang , Rosaura G. VidalMata , Manjushree Aithal , Nikhil Ambha Madhusudhana , John Nicholson , Lichao Sun , Brian D. Davison

Proteins have evolved through mutations, amino acid substitutions, since life appeared on Earth, some 109 years ago. The study of these phenomena has been of particular significance because of their impact on protein stability, function,…

Biomolecules · Quantitative Biology 2023-10-25 Jorge A. Vila

Directed evolution is an iterative laboratory process of designing proteins with improved function by iteratively synthesizing new protein variants and evaluating their desired property with expensive and time-consuming biochemical…

Machine Learning · Computer Science 2025-09-08 Matouš Soldát , Jiří Kléma

Accurate prediction of protein stability changes upon single-site variations (DDG) is important for protein design, as well as our understanding of the mechanism of genetic diseases. The performance of high-throughput computational methods…

Biomolecules · Quantitative Biology 2018-09-28 Ludovica Montanucci , Pier Luigi Martelli , Nir Ben-Tal , Piero Fariselli

Antibody design, a crucial task with significant implications across various disciplines such as therapeutics and biology, presents considerable challenges due to its intricate nature. In this paper, we tackle antigen-specific antibody…

Biomolecules · Quantitative Biology 2024-10-29 Xiangxin Zhou , Dongyu Xue , Ruizhe Chen , Zaixiang Zheng , Liang Wang , Quanquan Gu

Protein mutations can significantly influence protein solubility, which results in altered protein functions and leads to various diseases. Despite of tremendous effort, machine learning prediction of protein solubility changes upon…

Biomolecules · Quantitative Biology 2023-11-06 JunJie Wee , Jiahui Chen , Kelin Xia , Guo-Wei Wei

A long-standing goal of machine-learning-based protein engineering is to accelerate the discovery of novel mutations that improve the function of a known protein. We introduce a sampling framework for evolving proteins in silico that…

Machine Learning · Computer Science 2023-04-10 Patrick Emami , Aidan Perreault , Jeffrey Law , David Biagioni , Peter C. St. John

Stabilizing proteins is a foundational step in protein engineering. However, the evolutionary pressure of all extant proteins makes identifying the scarce number of mutations that will improve thermodynamic stability challenging. Deep…

Biomolecules · Quantitative Biology 2023-11-01 Jeffrey Ouyang-Zhang , Daniel J. Diaz , Adam R. Klivans , Philipp Krähenbühl

Traditional drug design faces significant challenges due to inherent chemical and biological complexities, often resulting in high failure rates in clinical trials. Deep learning advancements, particularly generative models, offer potential…

Quantitative Methods · Quantitative Biology 2025-08-27 Mahsa Sheikholeslami , Navid Mazrouei , Yousof Gheisari , Afshin Fasihi , Matin Irajpour , Ali Motahharynia

Designing high-performance optical lenses entails exploring a high-dimensional, tightly constrained space of surface curvatures, glass choices, element thicknesses, and spacings. In practice, standard optimizers (e.g., gradient-based local…

Neural and Evolutionary Computing · Computer Science 2026-01-30 Kirill Antonov , Teus Tukker , Tiago Botari , Thomas H. W. Bäck , Anna V. Kononova , Niki van Stein

Accurate prediction and optimization of protein-protein binding affinity is crucial for therapeutic antibody development. Although machine learning-based prediction methods $\Delta\Delta G$ are suitable for large-scale mutant screening,…

Biomolecules · Quantitative Biology 2024-09-19 Kairi Furui , Masahito Ohue

Cancer and its subtypes constitute approximately 30% of all causes of death globally and display a wide range of heterogeneity in terms of clinical and molecular responses to therapy. Molecular subtyping has enabled the use of precision…

Quantitative Methods · Quantitative Biology 2024-07-11 Anwar Khan , Boreom Lee

Proteins are shaped by gradual evolution under biophysical and functional constraints. Protein language models learn rich evolutionary constraints from large-scale sequences, and discrete diffusion-based protein language models~(\eg, DPLMs)…

Machine Learning · Computer Science 2026-05-14 Xinyou Wang , Liang Hong , Jiasheng Ye , Zaixiang Zheng , Yu Li , Shujian Huang , Quanquan Gu

Deep learning methods can struggle to handle domain shifts not seen in training data, which can cause them to not generalize well to unseen domains. This has led to research attention on domain generalization (DG), which aims to the model's…

Machine Learning · Computer Science 2022-05-10 Wei Zhu , Le Lu , Jing Xiao , Mei Han , Jiebo Luo , Adam P. Harrison

In real-world decision-making, uncertainty is important yet difficult to handle. Stochastic dominance provides a theoretically sound approach for comparing uncertain quantities, but optimization with stochastic dominance constraints is…

Machine Learning · Statistics 2023-02-28 Hanjun Dai , Yuan Xue , Niao He , Bethany Wang , Na Li , Dale Schuurmans , Bo Dai

The paper presents a novel deep learning approach, which extracts latent information from trained Deep Neural Networks (DNNs) and derives concise representations that are analyzed in an effective, unified way for prediction purposes. It is…

Machine Learning · Computer Science 2020-09-22 D. Kollias , N. Bouas , Y. Vlaxos , V. Brillakis , M. Seferis , I. Kollia , L. Sukissian , J. Wingate , S. Kollias

Predicting the docking between proteins and ligands is a crucial and challenging task for drug discovery. However, traditional docking methods mainly rely on scoring functions, and deep learning-based docking approaches usually neglect the…

Biomolecules · Quantitative Biology 2026-01-06 Yiqiang Yi , Xu Wan , Yatao Bian , Le Ou-Yang , Peilin Zhao
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