Related papers: Ludwig-Soret microscopy with vibrational photother…
We report a confocal interferometric mid-infrared photothermal (MIP) microscope and its application to label-free detection of biological nanoparticles down to single virus level. We apply the interferometric scattering principle to detect…
Label-free vibrational microscopy provides chemically specific access to cellular structure, yet quantitative volumetric chemical dynamics in living cells remain largely inaccessible, particularly on subsecond timescales relevant to…
Nonlinear optical methods, such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), are able to perform label free imaging, with chemical bonds specificity. Here, we demonstrate that the use of circularly…
Three-dimensional molecular imaging of living cells is essential for unraveling cellular metabolism and response to therapies. However, existing volumetric methods, including fluorescence microscopy and quantitative phase imaging, either…
Deep-tissue chemical imaging plays a vital role in biological and medical applications. Here, we present a shortwave infrared photothermal (SWIP) microscope for millimeter-deep vibrational imaging with sub-micron lateral resolution and…
Label-free optical imaging is valuable in biology and medicine with its non-destructive property and reduced optical and chemical damages. Quantitative phase (QPI) and molecular vibrational imaging (MVI) are the two most successful…
Interferometric scattering microscopy has been a very promising technology for highly sensitive label-free imaging of a broad spectrum of biological nanoparticles from proteins to viruses in a high-throughput manner. Although it can reveal…
Histological visualizations are critical to clinical disease management and are fundamental to biological understanding. However, current approaches that rely on bright-field microscopy require extensive tissue preparation prior to imaging.…
Infrared (IR) imaging has become a viable tool for visualizing various chemical bonds in a specimen. The performance, however, is limited in terms of spatial resolution and imaging speed. Here, instead of measuring the loss of the IR beam,…
Label-free optical microscopy through absorption or scattering spectroscopy provides fundamental insights across biology and materials science, yet its sensitivity remains fundamentally limited by photon shot noise. While recent…
Single-molecule detection enables direct observation of individual biomolecular events, providing mechanistic insights into biological processes and offering a powerful tool for disease diagnostics. However, the fundamental scale mismatch…
Vibrational spectroscopy, comprised of infrared absorption and Raman scattering spectroscopy, is widely used for label-free optical sensing and imaging in various scientific and industrial fields. The group theory states that the two…
Phase-contrast microscopy converts the optical phase introduced by transparent, unlabeled specimens into modulation in the intensity image. Modern phase imaging techniques are capable of quantifying phase shift at each point in the field of…
Mid-infrared photothermal (MIP) microscopy has been a promising label-free chemical imaging technique for functional characterization of specimens owing to its enhanced spatial resolution and high specificity. Recently developed wide-field…
This work presents a high-resolution X-ray microtomography system that uses commercial off-the-shelf (COTS) CMOS image sensors as direct detectors, relying on the sensor s intrinsic resolution to achieve tomographic reconstructions without…
Vascular imaging is critical for understanding human health and disease. Most established non-contact and label-free optical techniques capture predominantly structural information about vasculature. However, in many pathologies, functional…
Quantitative phase imaging (QPI) quantifies the sample-specific optical-phase-delay enabling objective studies of optically-transparent specimens such as biological samples, but lacks chemical sensitivity limiting its application to…
Label-free detection techniques for single particles and molecules play an important role in basic science, disease diagnostics, and nanomaterial investigations. While traditional fluorescence-based methods offer powerful tools for single…
Photothermal microscopy is an emerging tool for measuring light-matter interactions with single-molecule sensitivity. It is generally believed that the spectral acquisition speed in photothermal microscopy is limited by the slow thermal…
More than twenty years ago, scientists succeeded in pushing the limits of optical detection to single molecules using fluorescence. This breakthrough has revolutionized biophysical measurements, but restrictions in photophysics and labeling…