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This tutorial covers biomedical image reconstruction, from the foundational concepts of system modeling and direct reconstruction to modern sparsity and learning-based approaches. Imaging is a critical tool in biological research and…

Image and Video Processing · Electrical Eng. & Systems 2021-03-12 Michael T. McCann , Michael Unser

Recent studies suggest a potential link between the physical structure of mitochondria and neurodegenerative diseases. With advances in Electron Microscopy techniques, it has become possible to visualize the boundary and internal membrane…

Computer Vision and Pattern Recognition · Computer Science 2025-02-11 Faris Serdar Taşel , Efe Çiftci

The fast-growing field of soft matter research requires increasingly sophisticated tools for experimental studies. One of the oldest and most widely used tools to study soft matter systems is optical microscopy. Recent advances in optical…

Soft Condensed Matter · Physics 2011-08-17 Taewoo Lee , Bohdan Senyuk , Rahul P. Trivedi , Ivan I. Smalyukh

While electron microscopy offers crucial atomic-resolution insights into structure-property relationships, radiation damage severely limits its use on beam-sensitive materials like proteins and 2D materials. To overcome this challenge, we…

Computer Vision and Pattern Recognition · Computer Science 2025-07-18 Zifei Wang , Zian Mao , Xiaoya He , Xi Huang , Haoran Zhang , Chun Cheng , Shufen Chu , Tingzheng Hou , Xiaoqin Zeng , Yujun Xie

Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently…

Biological Physics · Physics 2017-05-02 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

Optics · Physics 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

Cutting edge deep learning techniques allow for image segmentation with great speed and accuracy. However, application to problems in materials science is often difficult since these complex models may have difficultly learning physical…

Image and Video Processing · Electrical Eng. & Systems 2019-12-13 James P. Horwath , Dmitri N. Zakharov , Remi Megret , Eric A. Stach

We present a deep learning driven computational approach to overcome the limitations of self-interference digital holography that imposed by inferior axial imaging performances. We demonstrate a 3D deep neural network model can…

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in…

Detailed 3D reconstruction is an important challenge with application to robotics, augmented and virtual reality, which has seen impressive progress throughout the past years. Advancements were driven by the availability of depth cameras…

Computer Vision and Pattern Recognition · Computer Science 2019-08-13 Andrea Nicastro , Ronald Clark , Stefan Leutenegger

Extracting reliable and quantitative microstructure information of living tissue by non-invasive imaging is an outstanding challenge for understanding disease mechanisms and allowing early stage diagnosis of pathologies. Magnetic Resonance…

Medical Physics · Physics 2021-07-12 Milena Capiglioni , Analia Zwick , Pablo Jimenez , Gonzalo A. Alvarez

Recent innovations in light sheet microscopy, paired with developments in tissue clearing techniques, enable the 3D imaging of large mammalian tissues with cellular resolution. Combined with the progress in large-scale data analysis, driven…

This paper describes the setup of two different solutions for laboratory X-ray microscopy working with geometric magnification. One setup uses thin-film transmission targets with an optimized tungsten-layer thickness and the electron gun…

Instrumentation and Detectors · Physics 2015-06-11 Thomas Ebensperger , Philipp Stahlhut , Frank Nachtrab , Simon Zabler , Randolf Hanke

Three-dimensional (3D) fluorescence imaging provides a vital approach for study of biological tissues with intricate structures, and optical sectioning structured illumination microscopy (OS-SIM) stands out for its high imaging speed, low…

Prior work on the Image Quality Transfer on Diffusion MRI (dMRI) has shown significant improvement over traditional interpolation methods. However, the difficulty in obtaining ultra-high resolution Diffusion MRI scans poses a problem in…

Image super-resolution technology is the process of obtaining high-resolution images from one or more low-resolution images. With the development of deep learning, image super-resolution technology based on deep learning method is emerging.…

Computer Vision and Pattern Recognition · Computer Science 2022-01-26 Fangyuan Zhu

By their very nature microscopy images of cells and tissues consist of a limited number of object types or components. In contrast to most natural scenes, the composition is known a priori. Decomposing biological images into semantically…

Computer Vision and Pattern Recognition · Computer Science 2019-09-04 Avelino Javer , Jens Rittscher

Medical image segmentation - the prerequisite of numerous clinical needs - has been significantly prospered by recent advances in convolutional neural networks (CNNs). However, it exhibits general limitations on modeling explicit long-range…

Computer Vision and Pattern Recognition · Computer Science 2021-07-13 Yundong Zhang , Huiye Liu , Qiang Hu

A three-dimensional cell culture called a spheroid serves as a foundational entity in a wide variety of modern tissue engineering applications, including 3D-bioprinting and preclinical drug testing. Lack of oxygen within tissue spheroids…

Computational Physics · Physics 2025-02-05 Katherine Vilinski-Mazur , Bogdan Kirillov , Oleg Rogozin , Dmitry Kolomenskiy

Recent years have witnessed the tremendous development of fusing fiber-optic imaging with supervised deep learning to enable high-quality imaging of hard-to-reach areas. Nevertheless, the supervised deep learning method imposes strict…