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Structured illumination microscopy (SIM) has emerged as an essential technique for 3D and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM,…

Modeling the 3D structures of cells and tissues is crucial in biology. Sequential cross-sectional images from electron microscopy provide high-resolution intracellular structure information. The segmentation of complex cell structures…

Quantitative Methods · Quantitative Biology 2025-02-14 Jin Kousaka , Atsuko H. Iwane , Yuichi Togashi

Recent advances in electron microscopy have enabled the imaging of single cells in 3D at nanometer length scale resolutions. An uncharted frontier for in silico biology is the ability to simulate cellular processes using these observed…

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going "beyond the diffraction barrier" comes at a price since most far-field super-resolution imaging techniques…

Diffusion magnetic resonance imaging (dMRI) is a crucial non-invasive technique for exploring the microstructure of the living human brain. Traditional hand-crafted and model-based tissue microstructure reconstruction methods often require…

Image and Video Processing · Electrical Eng. & Systems 2025-02-26 Xinrui Ma , Jian Cheng , Wenxin Fan , Ruoyou Wu , Yongquan Ye , Shanshan Wang

Microstructures, characterized by intricate structures at the microscopic scale, hold the promise of important disruptions in the field of mechanical engineering due to the superior mechanical properties they offer. One fundamental…

Computational Geometry · Computer Science 2024-11-26 Qiang Zou , Guoyue Luo

Despite advances in deep learning, robustness under domain shift remains a major bottleneck in medical imaging settings. Findings on natural images suggest that deep neural models can show a strong textural bias when carrying out image…

Image and Video Processing · Electrical Eng. & Systems 2021-06-29 Seoin Chai , Daniel Rueckert , Ahmed E. Fetit

The invention of three-dimensional printers has led to major innovations in tissue engineering. They have enabled the printing of complex geometries such as those that occur in natural tissues, that were not possible with traditional…

Quantitative Methods · Quantitative Biology 2015-11-23 Ali Bakhshinejad

This review paper delves into the present state of medical imaging, with a specific focus on the use of deep learning techniques for brain image synthesis. The need for medical image synthesis to improve diagnostic accuracy and decrease…

Image and Video Processing · Electrical Eng. & Systems 2023-09-20 Shubham Singh , Ammar Ranapurwala , Mrunal Bewoor , Sheetal Patil , Satyam Rai

3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…

Image and Video Processing · Electrical Eng. & Systems 2019-11-28 Yang Jiao , Mo Weng , Mei Yang

Lightsheet microscopy is a powerful 3-D imaging technique that addresses limitations of traditional optical and confocal microscopy but suffers from a low penetration depth and reduced image quality at greater depths. Multiview lightsheet…

Image and Video Processing · Electrical Eng. & Systems 2025-03-21 Marek Wodzinski , Henning Müller

Cells segmentation shows rapid growth in biology. Indeed, using the classical segmentation methods only is not enough to segment this type of images. In this manuscript, we will present a new method of ribosomes segmentation. A…

Image and Video Processing · Electrical Eng. & Systems 2021-01-14 Mariam El Oussini

Epithelial cells form diverse structures from squamous spherical organoids to densely packed pseudostratified tissues. Quantification of cellular properties in these contexts requires high-resolution deep imaging and computational…

Quantitative Methods · Quantitative Biology 2025-02-03 G. Paci , P. Vicente-Munuera , I. Fernandez-Mosquera , A. Miranda , K. Lau , Q. Zhang , R. Barrientos , Y. Mao

A central problem in neuroscience is reconstructing neuronal circuits on the synapse level. Due to a wide range of scales in brain architecture such reconstruction requires imaging that is both high-resolution and high-throughput. Existing…

Computer Vision and Pattern Recognition · Computer Science 2012-10-03 Tao Hu , Juan Nunez-Iglesias , Shiv Vitaladevuni , Lou Scheffer , Shan Xu , Mehdi Bolorizadeh , Harald Hess , Richard Fetter , Dmitri Chklovskii

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

Computer Vision and Pattern Recognition · Computer Science 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

Imaging dynamics of cellular morphogenesis with high spatial-temporal resolution in 3D is challenging, due to the low spatial resolution along the optical axis and photo-toxicity. However, some cellular structures are planar and hence 2D…

Quantitative Methods · Quantitative Biology 2020-07-14 Alka Bhat , Linjie Lu , Chen-Ho Wang , Simon Lo Vecchio , Riccardo Maraspini , Alf Honigmann , Daniel Riveline

The technique presented here identifies tethered mould designs, optimised for growing cultured tissue with very highly-aligned cells. It is based on a microscopic biophysical model for polarised cellular hydrogels. There is an unmet need…

Biological Physics · Physics 2023-06-26 James P. Hague , Allison E. Andrews , Hugh Dickinson

Medical imaging is an invaluable resource in medicine as it enables to peer inside the human body and provides scientists and physicians with a wealth of information indispensable for understanding, modelling, diagnosis, and treatment of…

Image and Video Processing · Electrical Eng. & Systems 2022-08-25 Hanene Ben Yedder , Ben Cardoen , Ghassan Hamarneh

We report on the 3-dimensional imaging of biological cells including living neurons by a recently developed fibered interferometric Scanning Optical Microscope. The topography and surface structure of mouse fibroblasts and hippocampal…

Optical microscopy has so far been restricted to superficial layers, leaving many important biological questions unanswered. Random scattering causes the ballistic focus, which is conventionally used for image formation, to decay…

Optics · Physics 2012-08-28 Ke Si , Reto Fiolka , Meng Cui