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Label-free single-cell imaging offers a scalable, non-invasive alternative to fluorescence-based cytometry, yet inferring molecular phenotypes directly from bright-field morphology remains challenging. We present a unified Deep Learning…

Computer Vision and Pattern Recognition · Computer Science 2026-05-15 Saqib Nazir , Ardhendu Behera

Assessing the quality of bovine satellite cells (BSCs) is essential for the cultivated meat industry, which aims to address global food sustainability challenges. This study aims to develop a label-free method for predicting fluorescence…

Computer Vision and Pattern Recognition · Computer Science 2024-10-18 Sania Sinha , Aarham Wasit , Won Seob Kim , Jongkyoo Kim , Jiyoon Yi

Despite fluorescent cell-labelling being widely employed in biomedical studies, some of its drawbacks are inevitable, with unsuitable fluorescent probes or probes inducing a functional change being the main limitations. Consequently, the…

Quantitative Methods · Quantitative Biology 2020-03-03 Yan Ge , Philipp Rosendahl , Claudio Durán , Nicole Töpfner , Sara Ciucci , Jochen Guck , Carlo Vittorio Cannistraci

Live cell culture is crucial in biomedical studies for analyzing cell properties and dynamics in vitro. This study focuses on segmenting unstained live cells imaged with bright-field microscopy. While many segmentation approaches exist for…

Quantitative Methods · Quantitative Biology 2025-08-26 Surajit Das , Gourav Roy , Pavel Zun

Automated semantic segmentation of cell nuclei in microscopic images is crucial for disease diagnosis and tissue microenvironment analysis. Nonetheless, this task presents challenges due to the complexity and heterogeneity of cells. While…

Image and Video Processing · Electrical Eng. & Systems 2023-08-10 Zhuchen Shao , Sourya Sengupta , Hua Li , Mark A. Anastasio

Primary neuronal cultures have been widely used to study neuronal morphology, neurophysiology, neurodegenerative processes, and molecular mechanism of synaptic plasticity underlying learning and memory. Yet, the unique behavioral properties…

Image and Video Processing · Electrical Eng. & Systems 2020-08-04 Mikhail E. Kandel , Eunjae Kim , Young Jae Lee , Gregory Tracy , Hee Jung Chung , Gabriel Popescu

Recent progress with conditional image diffusion models has been stunning, and this holds true whether we are speaking about models conditioned on a text description, a scene layout, or a sketch. Unconditional image diffusion models are…

Computer Vision and Pattern Recognition · Computer Science 2023-06-22 William Harvey , Frank Wood

Motion models (i.e., transition probability densities) are often deduced from fluorescence widefield tracking experiments by analyzing single-particle trajectories post-processed from data. This analysis immediately raises the question: To…

Biological Physics · Physics 2025-07-29 Zachary H. Hendrix , Lance W. Q. Xu , Steve Pressé

Accurate single cell detection in brightfield microscopy is crucial for biological research, yet data scarcity and annotation bottlenecks limit the progress of deep learning methods. We investigate the use of unconditional models to…

Computer Vision and Pattern Recognition · Computer Science 2025-12-02 Mario de Jesus da Graca , Jörg Dahlkemper , Peer Stelldinger

In life sciences, fluorescent labeling techniques are used to study molecular structures and interactions of cells. However, this type of cell imaging has its own limitations, one of which is that the process of staining the cells could be…

Image and Video Processing · Electrical Eng. & Systems 2020-05-07 Eunjae Kim

Accurate segmentation of multiple organs in Computed Tomography (CT) images plays a vital role in computer-aided diagnosis systems. While various supervised learning approaches have been proposed recently, these methods heavily depend on a…

Computer Vision and Pattern Recognition · Computer Science 2025-03-21 Yongzhi Huang , Fengjun Xi , Liyun Tu , Jinxin Zhu , Haseeb Hassan , Liyilei Su , Yun Peng , Jingyu Li , Jun Ma , Bingding Huang

Label-free imaging has gained broad interest because of its potential to omit elaborate staining procedures which is especially relevant for in vivo use. Label-free multiphoton microscopy (MPM), for instance, exploits two-photon excitation…

Determining the trajectories of cells and their lineages or ancestries in live-cell experiments are fundamental to the understanding of how cells behave and divide. This paper proposes novel online algorithms for jointly tracking and…

Signal Processing · Electrical Eng. & Systems 2021-10-29 Tran Thien Dat Nguyen , Ba-Ngu Vo , Ba-Tuong Vo , Du Yong Kim , Yu Suk Choi

Vision foundation models trained on discretely sampled images achieve strong performance on classification benchmarks, yet whether their representations encode the continuous processes underlying their training data remains unclear. This…

Computer Vision and Pattern Recognition · Computer Science 2026-01-30 Pritika Vig , Ren-Chin Wu , William Lotter

Fluorescence labeling is the standard approach to reveal cellular structures and other subcellular constituents for microscopy images. However, this invasive procedure may perturb or even kill the cells and the procedure itself is highly…

Image and Video Processing · Electrical Eng. & Systems 2024-06-25 Han Liu , Hao Li , Jiacheng Wang , Yubo Fan , Zhoubing Xu , Ipek Oguz

Understanding non-genetic determinants of cell fate is critical for developing and improving cancer therapies, as genetically identical cells can exhibit divergent outcomes under the same treatment conditions. In this work, we present a…

Computer Vision and Pattern Recognition · Computer Science 2026-03-18 Florian Bürger , Martim Dias Gomes , Adrián E. Granada , Noémie Moreau , Katarzyna Bozek

Fluorescence microscopy images contain several channels, each indicating a marker staining the sample. Since many different marker combinations are utilized in practice, it has been challenging to apply deep learning based segmentation…

Computer Vision and Pattern Recognition · Computer Science 2021-01-28 Alvaro Gomariz , Raphael Egli , Tiziano Portenier , César Nombela-Arrieta , Orcun Goksel

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

Exposure to intense illumination light is an unavoidable consequence of fluorescence microscopy, and poses a risk to the health of the sample in every live-cell fluorescence microscopy experiment. Furthermore, the possible side-effects of…

Computer Vision and Pattern Recognition · Computer Science 2017-01-24 David Richmond , Anna Payne-Tobin Jost , Talley Lambert , Jennifer Waters , Hunter Elliott

Identifying subtle phenotypic variations in cellular images is critical for advancing biological research and accelerating drug discovery. These variations are often masked by the inherent cellular heterogeneity, making it challenging to…

Computer Vision and Pattern Recognition · Computer Science 2026-01-28 Anis Bourou , Biel Castaño Segade , Thomas Boyer , Valérie Mezger , Auguste Genovesio
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