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We present a weakly supervised deep learning method to perform instance segmentation of cells present in microscopy images. Annotation of biomedical images in the lab can be scarce, incomplete, and inaccurate. This is of concern when…

Computer Vision and Pattern Recognition · Computer Science 2019-08-28 Fidel A. Guerrero-Peña , Pedro D. Marrero Fernandez , Tsang Ing Ren , Alexandre Cunha

Diffusion models are a special type of generative model, capable of synthesising new data from a learnt distribution. We introduce DISPR, a diffusion-based model for solving the inverse problem of three-dimensional (3D) cell shape…

Computer Vision and Pattern Recognition · Computer Science 2023-03-15 Dominik J. E. Waibel , Ernst Röell , Bastian Rieck , Raja Giryes , Carsten Marr

Membrane phase-separation is a mechanism that biological membranes often use to locally concentrate specific lipid species in order to organize diverse membrane processes. Phase separation has also been explored as a tool for the design of…

Computational Physics · Physics 2020-06-26 Alexander Zhiliakov , Yifei Wang , Annalisa Quaini , Maxim Olshanskii , Sheereen Majd

We present cytometric classification of live healthy and cancer cells by using the spatial morphological and textural information found in the label-free quantitative phase images of the cells. We compare both healthy cells to primary tumor…

Quantitative Methods · Quantitative Biology 2020-01-23 Darina Roitshtain , Lauren Wolbromsky , Evgeny Bal , Hayit Greenspan , Lisa L. Satterwhite , Natan T. Shaked

3D spatial graphs play a crucial role in biological and clinical research by modeling anatomical networks such as blood vessels,neurons, and airways. However, generating 3D biological graphs while maintaining anatomical validity remains…

Computer Vision and Pattern Recognition · Computer Science 2025-07-08 Chinmay Prabhakar , Suprosanna Shit , Tamaz Amiranashvili , Hongwei Bran Li , Bjoern Menze

Whole-slide images (WSIs) from cancer patients contain rich information that can be used for medical diagnosis or to follow treatment progress. To automate their analysis, numerous deep learning methods based on convolutional neural…

Computer Vision and Pattern Recognition · Computer Science 2026-02-18 Lucas Sancéré , Noémie Moreau , Katarzyna Bozek

Staphylococcus aureus is an important pathogen, giving rise to antimicrobial resistance in cell strains such as Methicillin Resistant S. aureus (MRSA). Here we report an image analysis framework for automated detection and image…

Biological Physics · Physics 2016-10-21 Adam J. M. Wollman , Helen Miller , Simon Foster , Mark C. Leake

Tomographic imaging reveals internal structures of 3D objects and is crucial for medical diagnoses. Visualizing the morphology and appearance of non-planar sparse anatomical structures that extend over multiple 2D slices in tomographic…

Computer Vision and Pattern Recognition · Computer Science 2024-11-28 Leonhard Rist , Pluvio Stephan , Noah Maul , Linda Vorberg , Hendrik Ditt , Michael Sühling , Andreas Maier , Bernhard Egger , Oliver Taubmann

A detailed understanding of the interface between living cells and substrate materials is of rising importance in many fields of medicine, biology and biotechnology. Cells at interfaces often form epithelia. The physical barrier that they…

Cellular structures manifest their outstanding mechanical properties in many biological systems. One key challenge for designing and optimizing these geometrically complicated structures lies in devising an effective geometric…

3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…

Image and Video Processing · Electrical Eng. & Systems 2019-11-28 Yang Jiao , Mo Weng , Mei Yang

The development of single-cell and spatial transcriptomics has revolutionized our capacity to investigate cellular properties, functions, and interactions in both cellular and spatial contexts. However, the analysis of single-cell and…

Genomics · Quantitative Biology 2024-12-09 Shuang Ge , Shuqing Sun , Huan Xu , Qiang Cheng , Zhixiang Ren

An automatic evidential segmentation method based on Dempster-Shafer theory and deep learning is proposed to segment lymphomas from three-dimensional Positron Emission Tomography (PET) and Computed Tomography (CT) images. The architecture…

Computer Vision and Pattern Recognition · Computer Science 2022-11-21 Ling Huang , Su Ruan , Pierre Decazes , Thierry Denoeux

This report describes the design, implementation, evaluation and original enhancements to the Live-Wire method for 2D and 3D image segmentation. Live-Wire 2D employs a semi-automatic paradigm; the user is asked to select a few boundary…

Computer Vision and Pattern Recognition · Computer Science 2013-06-17 Ognjen Arandjelovic

It is widely recognized that the shape of epithelial cells is determined by the tension generated by the actomyosin cortex and the adhesion of cells to the substrate and to each other. To account for these biological and structural…

Biological Physics · Physics 2021-08-11 Nicolas Harmand , Sylvie Hénon

We systematically evaluate a Deep Learning (DL) method in a 3D medical image segmentation task. Our segmentation method is integrated into the radiosurgery treatment process and directly impacts the clinical workflow. With our method, we…

Image and Video Processing · Electrical Eng. & Systems 2021-08-24 Boris Shirokikh , Alexandra Dalechina , Alexey Shevtsov , Egor Krivov , Valery Kostjuchenko , Amayak Durgaryan , Mikhail Galkin , Andrey Golanov , Mikhail Belyaev

Wood comprises different cell types, such as fibers, tracheids and vessels, defining its properties. Studying cells' shape, size, and arrangement in microscopy images is crucial for understanding wood characteristics. Typically, this…

Computer Vision and Pattern Recognition · Computer Science 2024-06-19 Saqib Qamar , Abu Imran Baba , Stéphane Verger , Magnus Andersson

Mapping conformational heterogeneity of macromolecules presents a formidable challenge to X-ray crystallography and cryo-electron microscopy, which often presume its absence. This has severely limited our knowledge of the conformations…

Biological Physics · Physics 2015-05-14 P. Schwander , R. Fung , G. N. Phillips , A. Ourmazd

Cell shape analysis is important in biomedical research. Deep learning methods may perform to segment individual cells if they use sufficient training data that the boundary of each cell is annotated. However, it is very time-consuming for…

Image and Video Processing · Electrical Eng. & Systems 2020-02-26 Kazuya Nishimura , Dai Fei Elmer Ker , Ryoma Bise

Reliable cell segmentation and classification from biomedical images is a crucial step for both scientific research and clinical practice. A major challenge for more robust segmentation and classification methods is the large variations in…

Cell Behavior · Quantitative Biology 2017-10-31 Mo Zhang , Xiang Li , Mengjia Xu , Quanzheng Li