English
Related papers

Related papers: Single cell resolution 3D imaging and segmentation…

200 papers

Although cell shape can reflect the mechanical and biochemical properties of the cell and its environment, quantification of 3D cell shapes within 3D tissues remains difficult, typically requiring digital reconstruction from a stack of 2D…

Quantitative Methods · Quantitative Biology 2019-03-06 Tristan A. Sharp , Matthias Merkel , M. Lisa Manning , Andrea J. Liu

Cells are the fundamental unit of biological organization, and identifying them in imaging data - cell segmentation - is a critical task for various cellular imaging experiments. While deep learning methods have led to substantial progress…

Accurate segmentation of 3-D cell nuclei in microscopy images is essential for the study of nuclear organization, gene expression, and cell morphodynamics. Current image segmentation methods are challenged by the complexity and variability…

Image and Video Processing · Electrical Eng. & Systems 2022-03-18 Sundaresh Ram , Jeffrey J. Rodriguez

Analysis of microscopy images can provide insight into many biological processes. One particularly challenging problem is cell nuclear segmentation in highly anisotropic and noisy 3D image data. Manually localizing and segmenting each and…

Computer Vision and Pattern Recognition · Computer Science 2013-09-18 Christian Widmer , Philipp Drewe , Xinghua Lou , Shefali Umrania , Stephanie Heinrich , Gunnar Rätsch

Accurate segmentation of live cell images has broad applications in clinical and research contexts. Deep learning methods have been able to perform cell segmentations with high accuracy; however developing machine learning models to do this…

Image and Video Processing · Electrical Eng. & Systems 2022-12-06 Mayur Bhandary , J. Patricio Reyes , Eylul Ertay , Aman Panda

Imaging dynamics of cellular morphogenesis with high spatial-temporal resolution in 3D is challenging, due to the low spatial resolution along the optical axis and photo-toxicity. However, some cellular structures are planar and hence 2D…

Quantitative Methods · Quantitative Biology 2020-07-14 Alka Bhat , Linjie Lu , Chen-Ho Wang , Simon Lo Vecchio , Riccardo Maraspini , Alf Honigmann , Daniel Riveline

Lipid droplets (LDs) are subcellular organelles with important roles in lipid storage and metabolism and involved in various diseases including cancer, obesity, and diabetes. Conventional methods, however, have limited ability to provide…

Biological Physics · Physics 2016-11-08 Kyoohyun Kim , SeoEun Lee , Jonghee Yoon , JiHan Heo , Chulhee Choi , YongKeun Park

3D cell segmentation methods are often hindered by \emph{oversegmentation}, where a single cell is incorrectly split into multiple fragments. This degrades the final segmentation quality and is notoriously difficult to resolve, as…

Computer Vision and Pattern Recognition · Computer Science 2025-12-22 Peter Chen , Bryan Chang , Olivia A Creasey , Julie Beth Sneddon , Zev J Gartner , Yining Liu

Over the last two decades, scientific literature has been blooming with various means of simulating epithelial cell colonies. Each of these simulations can be separated by their respective efficiency (expressed in terms of consumed…

Computational Physics · Physics 2022-12-20 Kevin Höllring , Ana-Sunčana Smith

We present a novel method for characterizing the microstructure of a material from volumetric datasets such as 3D image data from computed tomography (CT). The method is based on a new statistical model for the distribution of voxel…

Materials Science · Physics 2021-01-06 Elise Otterlei Brenne , Vedrana Andersen Dahl , Peter Stanley Jørgensen

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in…

Single cell segmentation is critical and challenging in live cell imaging data analysis. Traditional image processing methods and tools require time-consuming and labor-intensive efforts of manually fine-tuning parameters. Slight variations…

Quantitative Methods · Quantitative Biology 2019-04-24 Weikang Wang , David A. Taft , Yi-Jiun Chen , Jingyu Zhang , Callen T. Wallace , Min Xu , Simon C. Watkins , Jianhua Xing

We measure different contributions to entropy production in a living functional epithelial tissue. We do this by extracting the functional dynamics of development while at the same time quantifying fluctuations. Using the translucent…

Soft Condensed Matter · Physics 2023-01-25 Mark Olenik , Jake Turley , Stephen Cross , Helen Weavers , Paul Martin , Isaac Chenchiah , Tanniemola Liverpool

A software for processing sets of full-color images of biological tissue histological sections is developed. We used histological sections obtained by the method of high-precision layer-by-layer grinding of frozen biological tissues. The…

Background Analyzing images to accurately estimate the number of different cell types in the brain using automatic methods is a major objective in neuroscience. The automatic and selective detection and segmentation of neurons would be an…

Image and Video Processing · Electrical Eng. & Systems 2024-10-07 Antonio LaTorre , Lidia Alonso-Nanclares , José María Peña , Javier De Felipe

Cells segmentation shows rapid growth in biology. Indeed, using the classical segmentation methods only is not enough to segment this type of images. In this manuscript, we will present a new method of ribosomes segmentation. A…

Image and Video Processing · Electrical Eng. & Systems 2021-01-14 Mariam El Oussini

Deep learning has revolutionized medical and biological imaging, particularly in segmentation tasks. However, segmenting biological cells remains challenging due to the high variability and complexity of cell shapes. Addressing this…

Computer Vision and Pattern Recognition · Computer Science 2025-06-03 Tianhao Zhang , Heather J. McCourty , Berardo M. Sanchez-Tafolla , Anton Nikolaev , Lyudmila S. Mihaylova

In this work, the possibilities for segmentation of cells from their background and each other in digital image were tested, combined and improoved. Lot of images with young, adult and mixture cells were able to prove the quality of…

Computer Vision and Pattern Recognition · Computer Science 2011-01-13 Jan Urban

We investigate morphologies of proliferating cellular tissue using a newly developed numerical simulation model for mechanical cell division. The model reproduces structures of simple multi-cellular organisms via simple rules for selective…

Soft Condensed Matter · Physics 2018-11-20 Pranav Madhikar , Jan Åström , Björn Baumeier , Mikko Karttunen

The segmentation module which precisely outlines the nodules is a crucial step in a computer-aided diagnosis(CAD) system. The most challenging part of such a module is how to achieve high accuracy of the segmentation, especially for the…

Image and Video Processing · Electrical Eng. & Systems 2022-02-09 Xinliang Fu , Jiayin Zheng , Juanyun Mai , Yanbo Shao , Minghao Wang , Linyu Li , Zhaoqi Diao , Yulong Chen , Jianyu Xiao , Jian You , Airu Yin , Yang Yang , Xiangcheng Qiu , Jinsheng Tao , Bo Wang , Hua Ji