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Spatial transcriptomics (ST) reveals spatial heterogeneity of gene expression, yet its resolution is limited by current platforms. Recent methods enhance resolution via H&E-stained histology, but three major challenges persist: (1)…

Image and Video Processing · Electrical Eng. & Systems 2025-08-12 Xuepeng Liu , Zheng Jiang , Pinan Zhu , Hanyu Liu , Chao Li

The recent advancement of spatial transcriptomics (ST) allows to characterize spatial gene expression within tissue for discovery research. However, current ST platforms suffer from low resolution, hindering in-depth understanding of…

Image and Video Processing · Electrical Eng. & Systems 2025-11-05 Xiaofei Wang , Xingxu Huang , Stephen J. Price , Chao Li

Spatial transcriptomics (ST) is a groundbreaking genomic technology that enables spatial localization analysis of gene expression within tissue sections. However, it is significantly limited by high costs and sparse spatial resolution. An…

Image and Video Processing · Electrical Eng. & Systems 2024-07-31 Zhiceng Shi , Shuailin Xue , Fangfang Zhu , Wenwen Min

Spatial transcriptomics (ST) enables gene expression mapping within anatomical context but remains costly and low-throughput. Hematoxylin and eosin (H\&E) staining offers rich morphology yet lacks molecular resolution. We present…

Spatial Transcriptomics (ST) reveals the spatial distribution of gene expression in tissues, offering critical insights into biological processes and disease mechanisms. However, the high cost, limited coverage, and technical complexity of…

Computer Vision and Pattern Recognition · Computer Science 2025-04-22 Yi Niu , Jiashuai Liu , Yingkang Zhan , Jiangbo Shi , Di Zhang , Marika Reinius , Ines Machado , Mireia Crispin-Ortuzar , Jialun Wu , Chen Li , Zeyu Gao

Spatial Transcriptomics (ST) provides spatially resolved gene expression profiles within intact tissue architecture, enabling molecular analysis in histological context. However, the high cost, limited throughput, and restricted data…

Machine Learning · Computer Science 2026-03-31 Yaoyu Fang , Jiahe Qian , Xinkun Wang , Lee A. Cooper , Bo Zhou

The rapid development of spatial transcriptomics (ST) technologies is revolutionizing our understanding of the spatial organization of biological tissues. Current ST methods, categorized into next-generation sequencing-based (seq-based) and…

Machine Learning · Computer Science 2024-07-19 Xiaoyu Li , Fangfang Zhu , Wenwen Min

Spatial transcriptomics (ST) has emerged as a powerful technology for bridging histology imaging with gene expression profiling. However, its application has been limited by low throughput and the need for specialized experimental…

Computer Vision and Pattern Recognition · Computer Science 2025-06-09 Tinglin Huang , Tianyu Liu , Mehrtash Babadi , Wengong Jin , Rex Ying

Spatial transcriptomics (ST) technologies can be used to align transcriptomes with histopathological morphology, presenting exciting new opportunities for biomolecular discovery. Using ST data, we construct a novel framework, GeneFlow, to…

Quantitative Methods · Quantitative Biology 2025-11-04 Mengbo Wang , Shourya Verma , Aditya Malusare , Luopin Wang , Yiyang Lu , Vaneet Aggarwal , Mario Sola , Ananth Grama , Nadia Atallah Lanman

Spatial Transcriptomics (ST) merges the benefits of pathology images and gene expression, linking molecular profiles with tissue structure to analyze spot-level function comprehensively. Predicting gene expression from histology images is a…

Computer Vision and Pattern Recognition · Computer Science 2025-12-01 Chen Zhang , Yilu An , Ying Chen , Hao Li , Xitong Ling , Lihao Liu , Junjun He , Yuxiang Lin , Zihui Wang , Rongshan Yu

Spatial transcriptomics (ST) bridges gene expression and tissue morphology but faces clinical adoption barriers due to technical complexity and prohibitive costs. While computational methods predict gene expression from H&E-stained…

Computer Vision and Pattern Recognition · Computer Science 2025-11-20 Ziqiao Weng , Yaoyu Fang , Jiahe Qian , Xinkun Wang , Lee AD Cooper , Weidong Cai , Bo Zhou

Spatial transcriptomics (ST) enables the visualization of gene expression within the context of tissue morphology. This emerging discipline has the potential to serve as a foundation for developing tools to design precision medicines.…

Image and Video Processing · Electrical Eng. & Systems 2024-11-12 Shivam Kumar , Samrat Chatterjee

Spatial Transcriptomics (ST) offers spatially resolved gene expression but remains costly. Predicting expression directly from widely available Hematoxylin and Eosin (H&E) stained images presents a cost-effective alternative. However, most…

Computer Vision and Pattern Recognition · Computer Science 2025-10-07 Jiarui Ouyang , Yihui Wang , Yihang Gao , Yingxue Xu , Shu Yang , Hao Chen

High-resolution spatial transcriptomics (HR-ST) technologies offer unprecedented insights into tissue architecture but lack standardized frameworks for histological annotation. We present ST2HE, a cross-platform generative framework that…

Quantitative Methods · Quantitative Biology 2025-10-16 Zhentao Liu , Arun Das , Wen Meng , Yu-Chiao Chiu , Shou-Jiang Gao , Yufei Huang

Spatial transcriptomics (ST) provides essential spatial context by mapping gene expression within tissue, enabling detailed study of cellular heterogeneity and tissue organization. However, aligning ST data with histology images poses…

"Is it possible to predict expression levels of different genes at a given spatial location in the routine histology image of a tumor section by modeling its stain absorption characteristics?" In this work, we propose a "stain-aware"…

Image and Video Processing · Electrical Eng. & Systems 2021-08-27 Muhammad Dawood , Kim Branson , Nasir M. Rajpoot , Fayyaz ul Amir Afsar Minhas

Spatial transcriptomics offers spatially resolved gene expression profiling within tissue sections, but its cost and limited throughput hinder large-scale deployment. To extend this capability to routine practice, recent computational…

Machine Learning · Computer Science 2026-05-07 Keunho Byeon , Jin Tae Kwak

The rapid development of digital pathology and modern deep learning has facilitated the emergence of pathology foundation models that are expected to solve general pathology problems under various disease conditions in one unified model,…

Computer Vision and Pattern Recognition · Computer Science 2025-07-10 Yutong Sun , Sichen Zhu , Peng Qiu

Spatial Transcriptomics is a novel technology that aligns histology images with spatially resolved gene expression profiles. Although groundbreaking, it struggles with gene capture yielding high corruption in acquired data. Given potential…

Computer Vision and Pattern Recognition · Computer Science 2024-09-30 Gabriel Mejia , Daniela Ruiz , Paula Cárdenas , Leonardo Manrique , Daniela Vega , Pablo Arbeláez

While spatial transcriptomics (ST) has advanced our understanding of gene expression in tissue context, its high experimental cost limits its large-scale application. Predicting ST from pathology images is a promising, cost-effective…

Computer Vision and Pattern Recognition · Computer Science 2026-03-25 Zhiceng Shi , Changmiao Wang , Jun Wan , Wenwen Min
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