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We present the development of the first ultrafast transmission electron microscope (UTEM) driven by localized photoemission from a field emitter cathode. We describe the implementation of the instrument, the photoemitter concept and the…

We demonstrate fluorescence microscopy of individual fermionic potassium atoms in a 527-nm-period optical lattice. Using electromagnetically induced transparency (EIT) cooling on the 770.1-nm D$_1$ transition of $^{40}$K, we find that atoms…

Quantum Gases · Physics 2015-12-15 G. J. A. Edge , R. Anderson , D. Jervis , D. C. McKay , R. Day , S. Trotzky , J. H. Thywissen

Three-dimensional ultrasound localization microscopy (ULM) enables comprehensive visualization of the vasculature, thereby improving diagnostic reliability. Nevertheless, its clinical translation remains challenging, as the exponential…

Layered materials (LMs) are at the centre of an ever increasing research effort due to their potential use in a variety of applications. The presence of imperfections, such as bi- or multilayer areas, holes, grain boundaries, isotropic and…

Broadband absorption in the visible spectrum is essential in optoelectronic applications that involve power conversion such as photovoltaics and photocatalysis. Most ultrathin broadband absorbers use parasitic plasmonic structures that…

Confocal Raman microscopy offers a particular pathway for monitoring chemical "fingerprints" of intracellular components like the cell membrane, organelles, and nucleus. Nevertheless, conventional Raman acquisitions of fixed or randomly…

The usage of a GaAsP (gallium arsenide phosphide) photomultiplier for microscopical imaging allows the evaluation of low-light luminescent objects. We designed a setup for collecting a confocal microscopic image signal, which is divided…

We propose a computational imaging method for time-efficient light-field acquisition that combines a coded aperture with an event-based camera. Different from the conventional coded-aperture imaging method, our method applies a sequence of…

Computer Vision and Pattern Recognition · Computer Science 2024-11-21 Shuji Habuchi , Keita Takahashi , Chihiro Tsutake , Toshiaki Fujii , Hajime Nagahara

In vivo fluorescence imaging in the second near-infrared window (1.0-1.7 microns) can afford deep tissue penetration and high spatial resolution, owing to the reduced scattering of long-wavelength photons. Here, we synthesize a series of…

The concept of Wavelength Frame Multiplication (WFM) was developed to extend the usable wavelength range on long pulse neutron sources for instruments using pulse shaping choppers. For some instruments, it is combined with a pulse shaping…

Instrumentation and Detectors · Physics 2013-07-18 D. Nekrassov , C. Zendler , K. Lieutenant

Nonlinear structured illumination microscopy (nSIM) is an effective approach for super-resolution wide-field fluorescence microscopy with a theoretically unlimited resolution. In nSIM, carefully designed, highly-contrasted illumination…

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Lukas Harris , Micah Roschelle , Jack Bartley , Mekhail Anwar

We have developed a luminescence-measurement system for liquid bio/chemiluminescence that can obtain quantitative luminescence spectra as the absolute total number of luminescence photons at each wavelength or photon energy and quantum…

Quantitative Methods · Quantitative Biology 2007-05-23 Yoriko Ando , Kazuki Niwa , Nobuyuki Yamada , Tsutomu Irie , Toshiteru Enomoto , Hidehiro Kubota , Yoshihiro Ohmiya , Hidefumi Akiyama

We demonstrate the combination of a hemispherical solid immersion lens with a micro-photoluminescence setup. Two advantages introduced by the SIL, an improved resolution of 0.4 times the wavelength in vacuum and a 5 times enhancement of the…

Optics · Physics 2009-11-07 S. Moehl , Hui Zhao , B. Dal Don , S. Wachter , H. Kalt

Integral-field spectroscopy is the most effective method of exploiting the superb image quality of the ESO-VLT, allowing complex astrophysical processes to be probed on the angular scales currently accessible only for imaging data, but with…

We report on the use of silicon nitride (SiN) photonic integrated circuits (PICs) in high-value instrumentation, namely multi-color laser engines (MLEs), a core element of cutting-edge biophotonic systems applied to confocal microscopy,…

Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…

Microscopy has been key to tremendous advances in science, technology, and medicine, revealing structure and dynamics across time and length scales. However, combining high spatial and temporal resolution in a non-invasive, label-free…

Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the…

Convolutional networks require extensive image annotation, which can be costly and time-consuming. Feature Learning from Image Markers (FLIM) tackles this challenge by estimating encoder filters (i.e., kernel weights) from user-drawn…