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Condensed phase systems often exhibit a mixture of deterministic and stochastic dynamics at the nanoscale which are essential to understanding their function, but can be challenging to study directly using conventional imaging methods.…

Optics · Physics 2025-01-17 Arnab Sarkar , Allan S. Johnson

Quantum entanglement and squeezing have significantly improved phase estimation and imaging in interferometric settings beyond the classical limits. However, for a wide class of non-interferometric phase imaging/retrieval methods vastly…

Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…

Image and Video Processing · Electrical Eng. & Systems 2025-01-28 Tao Liu , Bingyang Wang , JiangTao Zhao , Maik Kahnt , Fucai Zhang

Quantitative phase imaging (QPI) is an emerging label-free technique that produces images containing morphological and dynamical information without contrast agents. Unfortunately, the phase is wrapped in most imaging system. Phase…

Image and Video Processing · Electrical Eng. & Systems 2021-09-01 Fangshu Yang , Thanh-an Pham , Nathalie Brandenberg , Matthias P. Lutolf , Jianwei Ma , Michael Unser

Imaging with optical resolution through and inside complex samples is a difficult challenge with important applications in many fields. The fundamental problem is that inhomogeneous samples, such as biological tissues, randomly scatter and…

Optics · Physics 2014-10-17 Ori Katz , Pierre Heidmann , Mathias Fink , Sylvain Gigan

Image segmentation is an important median level vision topic. Accurate and efficient multiphase segmentation for images with intensity inhomogeneity is still a great challenge. We present a new two-stage multiphase segmentation method…

Optimization and Control · Mathematics 2020-09-15 Xueyan Guo , Yunhua Xue , Chunlin Wu

Phase retrieval is the inverse problem of recovering a signal from magnitude-only Fourier measurements, and underlies numerous imaging modalities, such as Coherent Diffraction Imaging (CDI). A variant of this setup, known as holography,…

Machine Learning · Computer Science 2021-04-22 Hannah Lawrence , David A. Barmherzig , Henry Li , Michael Eickenberg , Marylou Gabrié

Holotomography, a three-dimensional quantitative phase imaging technique, presents an innovative, non-invasive approach to studying biological samples by exploiting the refractive index as an intrinsic imaging contrast. Despite offering…

Optics · Physics 2023-08-04 Herve Hugonnet , HyunJun Han , Weisun Park , YongKeun Park

Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

We present geometric-phase microscopy allowing a multipurpose quantitative phase imaging in which the ground-truth phase is restored by quantifying the phase retardance. The method uses broadband spatially incoherent light that is…

Fresnel incoherent correlation holography (FINCH) is a well-established digital holography technique for 3D imaging of objects illuminated by spatially incoherent light. FINCH has a higher lateral resolution of 1.5 times that of direct…

Digital holographic microcopy is a thriving imaging modality that attracts considerable research interest due to its ability to not only create excellent label-free contrast, but also supply valuable physical information regarding the…

Optics · Physics 2019-04-01 Gili Dardikman , Natan T. Shaked

A lensless digital holography enables wide-field microscopic imaging without the limitations imposed by optical lens performance. However, conventional holographic imaging often relies on magnifying optical systems to compensate for the low…

Optics · Physics 2025-06-24 Byung Gyu Chae

Quantitative phase microscopy (QPM) and interferometric scattering (iSCAT) microscopy are powerful label-free imaging techniques and are widely used for biomedical applications. Each method, however, possesses distinct limitations: QPM,…

Optics · Physics 2025-03-20 Kohki Horie , Keiichiro Toda , Takuma Nakamura , Takuro Ideguchi

Digital holography (DH) is a promising method for non-contact surface topography because the reconstructed phase image can visualize the nanometer unevenness in a sample. However, the axial range of this method is limited to the range of…

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

In this letter, a high-fidelity single-shot differential quantitative phase microscopy (dQPM) method is presented to effectively image nearly transparent biological samples. The proposed method is based on a common-path Sagnac…

Imaging through dynamic scattering media, such as biological tissue, presents a fundamental challenge due to light scattering and the formation of speckle patterns. These patterns not only degrade image quality but also decorrelate rapidly,…

Digital holography is one of the most widely used label-free microscopy techniques in biomedical imaging. Recovery of the missing phase information of a hologram is an important step in holographic image reconstruction. Here we demonstrate…

Image and Video Processing · Electrical Eng. & Systems 2021-05-28 Luzhe Huang , Tairan Liu , Xilin Yang , Yi Luo , Yair Rivenson , Aydogan Ozcan

We present a novel diffractive imaging method that harnesses a low-resolution real-space image to guide the phase retrieval. A computational algorithm is developed to utilize such prior knowledge as a real-space constraint in the iterative…

Optics · Physics 2020-10-27 Po-Nan Li , Soichi Wakatsuki , Piero A. Pianetta , Yijin Liu